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Whole Genome Sequencing in the Management of Non-Tuberculous Mycobacterial Infections

Infections caused by non-tuberculous mycobacteria (NTM) have been a public health problem in recent decades and contribute significantly to the clinical and economic burden globally. The diagnosis of infections is difficult and time-consuming and, in addition, the conventional diagnostics tests do n...

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Autores principales: Dohál, Matúš, Porvazník, Igor, Solovič, Ivan, Mokrý, Juraj
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8621650/
https://www.ncbi.nlm.nih.gov/pubmed/34835363
http://dx.doi.org/10.3390/microorganisms9112237
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author Dohál, Matúš
Porvazník, Igor
Solovič, Ivan
Mokrý, Juraj
author_facet Dohál, Matúš
Porvazník, Igor
Solovič, Ivan
Mokrý, Juraj
author_sort Dohál, Matúš
collection PubMed
description Infections caused by non-tuberculous mycobacteria (NTM) have been a public health problem in recent decades and contribute significantly to the clinical and economic burden globally. The diagnosis of infections is difficult and time-consuming and, in addition, the conventional diagnostics tests do not have sufficient discrimination power in species identification due to cross-reactions and not fully specific probes. However, technological advances have been made and the whole genome sequencing (WGS) method has been shown to be an essential part of routine diagnostics in clinical mycobacteriology laboratories. The use of this technology has contributed to the characterization of new species of mycobacteria, as well as the identification of gene mutations encoding resistance and virulence factors. Sequencing data also allowed to track global outbreaks of nosocomial NTM infections caused by M. abscessus complex and M. chimaera. To highlight the utility of WGS, we summarize recent scientific studies on WGS as a tool suitable for the management of NTM-induced infections in clinical practice.
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spelling pubmed-86216502021-11-27 Whole Genome Sequencing in the Management of Non-Tuberculous Mycobacterial Infections Dohál, Matúš Porvazník, Igor Solovič, Ivan Mokrý, Juraj Microorganisms Review Infections caused by non-tuberculous mycobacteria (NTM) have been a public health problem in recent decades and contribute significantly to the clinical and economic burden globally. The diagnosis of infections is difficult and time-consuming and, in addition, the conventional diagnostics tests do not have sufficient discrimination power in species identification due to cross-reactions and not fully specific probes. However, technological advances have been made and the whole genome sequencing (WGS) method has been shown to be an essential part of routine diagnostics in clinical mycobacteriology laboratories. The use of this technology has contributed to the characterization of new species of mycobacteria, as well as the identification of gene mutations encoding resistance and virulence factors. Sequencing data also allowed to track global outbreaks of nosocomial NTM infections caused by M. abscessus complex and M. chimaera. To highlight the utility of WGS, we summarize recent scientific studies on WGS as a tool suitable for the management of NTM-induced infections in clinical practice. MDPI 2021-10-27 /pmc/articles/PMC8621650/ /pubmed/34835363 http://dx.doi.org/10.3390/microorganisms9112237 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Dohál, Matúš
Porvazník, Igor
Solovič, Ivan
Mokrý, Juraj
Whole Genome Sequencing in the Management of Non-Tuberculous Mycobacterial Infections
title Whole Genome Sequencing in the Management of Non-Tuberculous Mycobacterial Infections
title_full Whole Genome Sequencing in the Management of Non-Tuberculous Mycobacterial Infections
title_fullStr Whole Genome Sequencing in the Management of Non-Tuberculous Mycobacterial Infections
title_full_unstemmed Whole Genome Sequencing in the Management of Non-Tuberculous Mycobacterial Infections
title_short Whole Genome Sequencing in the Management of Non-Tuberculous Mycobacterial Infections
title_sort whole genome sequencing in the management of non-tuberculous mycobacterial infections
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8621650/
https://www.ncbi.nlm.nih.gov/pubmed/34835363
http://dx.doi.org/10.3390/microorganisms9112237
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