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Genetic Characterization of Salmonella Infantis with Multiple Drug Resistance Profiles Isolated from a Poultry-Farm in Chile

Salmonella comprises over 2500 serotypes and foodborne contamination associated with this pathogen remains an important health concern worldwide. During the last decade, a shift in serotype prevalence has occurred as traditionally less prevalent serotypes are increasing in frequency of infections, e...

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Autores principales: Pardo-Esté, Coral, Lorca, Diego, Castro-Severyn, Juan, Krüger, Gabriel, Alvarez-Thon, Luis, Zepeda, Phillippi, Sulbaran-Bracho, Yoelvis, Hidalgo, Alejandro, Tello, Mario, Molina, Franck, Molina, Laurence, Remonsellez, Francisco, Castro-Nallar, Eduardo, Saavedra, Claudia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8621671/
https://www.ncbi.nlm.nih.gov/pubmed/34835497
http://dx.doi.org/10.3390/microorganisms9112370
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author Pardo-Esté, Coral
Lorca, Diego
Castro-Severyn, Juan
Krüger, Gabriel
Alvarez-Thon, Luis
Zepeda, Phillippi
Sulbaran-Bracho, Yoelvis
Hidalgo, Alejandro
Tello, Mario
Molina, Franck
Molina, Laurence
Remonsellez, Francisco
Castro-Nallar, Eduardo
Saavedra, Claudia
author_facet Pardo-Esté, Coral
Lorca, Diego
Castro-Severyn, Juan
Krüger, Gabriel
Alvarez-Thon, Luis
Zepeda, Phillippi
Sulbaran-Bracho, Yoelvis
Hidalgo, Alejandro
Tello, Mario
Molina, Franck
Molina, Laurence
Remonsellez, Francisco
Castro-Nallar, Eduardo
Saavedra, Claudia
author_sort Pardo-Esté, Coral
collection PubMed
description Salmonella comprises over 2500 serotypes and foodborne contamination associated with this pathogen remains an important health concern worldwide. During the last decade, a shift in serotype prevalence has occurred as traditionally less prevalent serotypes are increasing in frequency of infections, especially those related to poultry meat contamination. S. Infantis is one of the major emerging serotypes, and these strains commonly display antimicrobial resistance and can persist despite cleaning protocols. Thus, this work aimed to isolate S. Infantis strains from a poultry meat farm in Santiago, Chile and to characterize genetic variations present in them. We determined their genomic and phenotypic profiles at different points along the production line. The results indicate that the strains encompass 853 polymorphic sites (core-SNPs) with isolates differing from one another by 0–347 core SNPs, suggesting variation among them; however, we found discrete correlations with the source of the sample in the production line. Furthermore, the pan-genome was composed of 4854 total gene clusters of which 2618 (53.9%) corresponds to the core-genome and only 181 (3.7%) are unique genes (those present in one particular strain). This preliminary analysis will enrich the surveillance of Salmonella, yet further studies are required to assess their evolution and phylogeny.
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spelling pubmed-86216712021-11-27 Genetic Characterization of Salmonella Infantis with Multiple Drug Resistance Profiles Isolated from a Poultry-Farm in Chile Pardo-Esté, Coral Lorca, Diego Castro-Severyn, Juan Krüger, Gabriel Alvarez-Thon, Luis Zepeda, Phillippi Sulbaran-Bracho, Yoelvis Hidalgo, Alejandro Tello, Mario Molina, Franck Molina, Laurence Remonsellez, Francisco Castro-Nallar, Eduardo Saavedra, Claudia Microorganisms Article Salmonella comprises over 2500 serotypes and foodborne contamination associated with this pathogen remains an important health concern worldwide. During the last decade, a shift in serotype prevalence has occurred as traditionally less prevalent serotypes are increasing in frequency of infections, especially those related to poultry meat contamination. S. Infantis is one of the major emerging serotypes, and these strains commonly display antimicrobial resistance and can persist despite cleaning protocols. Thus, this work aimed to isolate S. Infantis strains from a poultry meat farm in Santiago, Chile and to characterize genetic variations present in them. We determined their genomic and phenotypic profiles at different points along the production line. The results indicate that the strains encompass 853 polymorphic sites (core-SNPs) with isolates differing from one another by 0–347 core SNPs, suggesting variation among them; however, we found discrete correlations with the source of the sample in the production line. Furthermore, the pan-genome was composed of 4854 total gene clusters of which 2618 (53.9%) corresponds to the core-genome and only 181 (3.7%) are unique genes (those present in one particular strain). This preliminary analysis will enrich the surveillance of Salmonella, yet further studies are required to assess their evolution and phylogeny. MDPI 2021-11-17 /pmc/articles/PMC8621671/ /pubmed/34835497 http://dx.doi.org/10.3390/microorganisms9112370 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pardo-Esté, Coral
Lorca, Diego
Castro-Severyn, Juan
Krüger, Gabriel
Alvarez-Thon, Luis
Zepeda, Phillippi
Sulbaran-Bracho, Yoelvis
Hidalgo, Alejandro
Tello, Mario
Molina, Franck
Molina, Laurence
Remonsellez, Francisco
Castro-Nallar, Eduardo
Saavedra, Claudia
Genetic Characterization of Salmonella Infantis with Multiple Drug Resistance Profiles Isolated from a Poultry-Farm in Chile
title Genetic Characterization of Salmonella Infantis with Multiple Drug Resistance Profiles Isolated from a Poultry-Farm in Chile
title_full Genetic Characterization of Salmonella Infantis with Multiple Drug Resistance Profiles Isolated from a Poultry-Farm in Chile
title_fullStr Genetic Characterization of Salmonella Infantis with Multiple Drug Resistance Profiles Isolated from a Poultry-Farm in Chile
title_full_unstemmed Genetic Characterization of Salmonella Infantis with Multiple Drug Resistance Profiles Isolated from a Poultry-Farm in Chile
title_short Genetic Characterization of Salmonella Infantis with Multiple Drug Resistance Profiles Isolated from a Poultry-Farm in Chile
title_sort genetic characterization of salmonella infantis with multiple drug resistance profiles isolated from a poultry-farm in chile
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8621671/
https://www.ncbi.nlm.nih.gov/pubmed/34835497
http://dx.doi.org/10.3390/microorganisms9112370
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