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The Development of Dual Vaccines against Lumpy Skin Disease (LSD) and Bovine Ephemeral Fever (BEF)
Dual vaccines (n = 6) against both lumpy skin disease (LSD) and bovine ephemeral fever (BEF) were constructed, based on the BEFV glycoprotein (G) gene, with or without the BEFV matrix (M) protein gene, inserted into one of two different LSDV backbones, nLSDV∆SOD-UCT or nLSDVSODis-UCT. The inserted g...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8621795/ https://www.ncbi.nlm.nih.gov/pubmed/34835146 http://dx.doi.org/10.3390/vaccines9111215 |
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author | Douglass, Nicola Omar, Ruzaiq Munyanduki, Henry Suzuki, Akiko de Moor, Warren Mutowembwa, Paidamwoyo Pretorius, Alri Nefefe, Tshifhiwa van Schalkwyk, Antoinette Kara, Pravesh Heath, Livio Williamson, Anna-Lise |
author_facet | Douglass, Nicola Omar, Ruzaiq Munyanduki, Henry Suzuki, Akiko de Moor, Warren Mutowembwa, Paidamwoyo Pretorius, Alri Nefefe, Tshifhiwa van Schalkwyk, Antoinette Kara, Pravesh Heath, Livio Williamson, Anna-Lise |
author_sort | Douglass, Nicola |
collection | PubMed |
description | Dual vaccines (n = 6) against both lumpy skin disease (LSD) and bovine ephemeral fever (BEF) were constructed, based on the BEFV glycoprotein (G) gene, with or without the BEFV matrix (M) protein gene, inserted into one of two different LSDV backbones, nLSDV∆SOD-UCT or nLSDVSODis-UCT. The inserted gene cassettes were confirmed by PCR; and BEFV protein was shown to be expressed by immunofluorescence. The candidate dual vaccines were initially tested in a rabbit model; neutralization assays using the South African BEFV vaccine (B-Phemeral) strain showed an African consensus G protein gene (Gb) to give superior neutralization compared to the Australian (Ga) gene. The two LSDV backbones expressing both Gb and M BEFV genes were tested in cattle and shown to elicit neutralizing responses to LSDV as well as BEFV after two inoculations 4 weeks apart. The vaccines were safe in cattle and all vaccinated animals were protected against virulent LSDV challenge, unlike a group of control naïve animals, which developed clinical LSD. Both neutralizing and T cell responses to LSDV were stimulated upon challenge. After two inoculations, all vaccinated animals produced BEFV neutralizing antibodies ≥ 1/20, which is considered protective for BEF. |
format | Online Article Text |
id | pubmed-8621795 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-86217952021-11-27 The Development of Dual Vaccines against Lumpy Skin Disease (LSD) and Bovine Ephemeral Fever (BEF) Douglass, Nicola Omar, Ruzaiq Munyanduki, Henry Suzuki, Akiko de Moor, Warren Mutowembwa, Paidamwoyo Pretorius, Alri Nefefe, Tshifhiwa van Schalkwyk, Antoinette Kara, Pravesh Heath, Livio Williamson, Anna-Lise Vaccines (Basel) Article Dual vaccines (n = 6) against both lumpy skin disease (LSD) and bovine ephemeral fever (BEF) were constructed, based on the BEFV glycoprotein (G) gene, with or without the BEFV matrix (M) protein gene, inserted into one of two different LSDV backbones, nLSDV∆SOD-UCT or nLSDVSODis-UCT. The inserted gene cassettes were confirmed by PCR; and BEFV protein was shown to be expressed by immunofluorescence. The candidate dual vaccines were initially tested in a rabbit model; neutralization assays using the South African BEFV vaccine (B-Phemeral) strain showed an African consensus G protein gene (Gb) to give superior neutralization compared to the Australian (Ga) gene. The two LSDV backbones expressing both Gb and M BEFV genes were tested in cattle and shown to elicit neutralizing responses to LSDV as well as BEFV after two inoculations 4 weeks apart. The vaccines were safe in cattle and all vaccinated animals were protected against virulent LSDV challenge, unlike a group of control naïve animals, which developed clinical LSD. Both neutralizing and T cell responses to LSDV were stimulated upon challenge. After two inoculations, all vaccinated animals produced BEFV neutralizing antibodies ≥ 1/20, which is considered protective for BEF. MDPI 2021-10-20 /pmc/articles/PMC8621795/ /pubmed/34835146 http://dx.doi.org/10.3390/vaccines9111215 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Douglass, Nicola Omar, Ruzaiq Munyanduki, Henry Suzuki, Akiko de Moor, Warren Mutowembwa, Paidamwoyo Pretorius, Alri Nefefe, Tshifhiwa van Schalkwyk, Antoinette Kara, Pravesh Heath, Livio Williamson, Anna-Lise The Development of Dual Vaccines against Lumpy Skin Disease (LSD) and Bovine Ephemeral Fever (BEF) |
title | The Development of Dual Vaccines against Lumpy Skin Disease (LSD) and Bovine Ephemeral Fever (BEF) |
title_full | The Development of Dual Vaccines against Lumpy Skin Disease (LSD) and Bovine Ephemeral Fever (BEF) |
title_fullStr | The Development of Dual Vaccines against Lumpy Skin Disease (LSD) and Bovine Ephemeral Fever (BEF) |
title_full_unstemmed | The Development of Dual Vaccines against Lumpy Skin Disease (LSD) and Bovine Ephemeral Fever (BEF) |
title_short | The Development of Dual Vaccines against Lumpy Skin Disease (LSD) and Bovine Ephemeral Fever (BEF) |
title_sort | development of dual vaccines against lumpy skin disease (lsd) and bovine ephemeral fever (bef) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8621795/ https://www.ncbi.nlm.nih.gov/pubmed/34835146 http://dx.doi.org/10.3390/vaccines9111215 |
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