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The Development of Dual Vaccines against Lumpy Skin Disease (LSD) and Bovine Ephemeral Fever (BEF)

Dual vaccines (n = 6) against both lumpy skin disease (LSD) and bovine ephemeral fever (BEF) were constructed, based on the BEFV glycoprotein (G) gene, with or without the BEFV matrix (M) protein gene, inserted into one of two different LSDV backbones, nLSDV∆SOD-UCT or nLSDVSODis-UCT. The inserted g...

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Autores principales: Douglass, Nicola, Omar, Ruzaiq, Munyanduki, Henry, Suzuki, Akiko, de Moor, Warren, Mutowembwa, Paidamwoyo, Pretorius, Alri, Nefefe, Tshifhiwa, van Schalkwyk, Antoinette, Kara, Pravesh, Heath, Livio, Williamson, Anna-Lise
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8621795/
https://www.ncbi.nlm.nih.gov/pubmed/34835146
http://dx.doi.org/10.3390/vaccines9111215
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author Douglass, Nicola
Omar, Ruzaiq
Munyanduki, Henry
Suzuki, Akiko
de Moor, Warren
Mutowembwa, Paidamwoyo
Pretorius, Alri
Nefefe, Tshifhiwa
van Schalkwyk, Antoinette
Kara, Pravesh
Heath, Livio
Williamson, Anna-Lise
author_facet Douglass, Nicola
Omar, Ruzaiq
Munyanduki, Henry
Suzuki, Akiko
de Moor, Warren
Mutowembwa, Paidamwoyo
Pretorius, Alri
Nefefe, Tshifhiwa
van Schalkwyk, Antoinette
Kara, Pravesh
Heath, Livio
Williamson, Anna-Lise
author_sort Douglass, Nicola
collection PubMed
description Dual vaccines (n = 6) against both lumpy skin disease (LSD) and bovine ephemeral fever (BEF) were constructed, based on the BEFV glycoprotein (G) gene, with or without the BEFV matrix (M) protein gene, inserted into one of two different LSDV backbones, nLSDV∆SOD-UCT or nLSDVSODis-UCT. The inserted gene cassettes were confirmed by PCR; and BEFV protein was shown to be expressed by immunofluorescence. The candidate dual vaccines were initially tested in a rabbit model; neutralization assays using the South African BEFV vaccine (B-Phemeral) strain showed an African consensus G protein gene (Gb) to give superior neutralization compared to the Australian (Ga) gene. The two LSDV backbones expressing both Gb and M BEFV genes were tested in cattle and shown to elicit neutralizing responses to LSDV as well as BEFV after two inoculations 4 weeks apart. The vaccines were safe in cattle and all vaccinated animals were protected against virulent LSDV challenge, unlike a group of control naïve animals, which developed clinical LSD. Both neutralizing and T cell responses to LSDV were stimulated upon challenge. After two inoculations, all vaccinated animals produced BEFV neutralizing antibodies ≥ 1/20, which is considered protective for BEF.
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spelling pubmed-86217952021-11-27 The Development of Dual Vaccines against Lumpy Skin Disease (LSD) and Bovine Ephemeral Fever (BEF) Douglass, Nicola Omar, Ruzaiq Munyanduki, Henry Suzuki, Akiko de Moor, Warren Mutowembwa, Paidamwoyo Pretorius, Alri Nefefe, Tshifhiwa van Schalkwyk, Antoinette Kara, Pravesh Heath, Livio Williamson, Anna-Lise Vaccines (Basel) Article Dual vaccines (n = 6) against both lumpy skin disease (LSD) and bovine ephemeral fever (BEF) were constructed, based on the BEFV glycoprotein (G) gene, with or without the BEFV matrix (M) protein gene, inserted into one of two different LSDV backbones, nLSDV∆SOD-UCT or nLSDVSODis-UCT. The inserted gene cassettes were confirmed by PCR; and BEFV protein was shown to be expressed by immunofluorescence. The candidate dual vaccines were initially tested in a rabbit model; neutralization assays using the South African BEFV vaccine (B-Phemeral) strain showed an African consensus G protein gene (Gb) to give superior neutralization compared to the Australian (Ga) gene. The two LSDV backbones expressing both Gb and M BEFV genes were tested in cattle and shown to elicit neutralizing responses to LSDV as well as BEFV after two inoculations 4 weeks apart. The vaccines were safe in cattle and all vaccinated animals were protected against virulent LSDV challenge, unlike a group of control naïve animals, which developed clinical LSD. Both neutralizing and T cell responses to LSDV were stimulated upon challenge. After two inoculations, all vaccinated animals produced BEFV neutralizing antibodies ≥ 1/20, which is considered protective for BEF. MDPI 2021-10-20 /pmc/articles/PMC8621795/ /pubmed/34835146 http://dx.doi.org/10.3390/vaccines9111215 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Douglass, Nicola
Omar, Ruzaiq
Munyanduki, Henry
Suzuki, Akiko
de Moor, Warren
Mutowembwa, Paidamwoyo
Pretorius, Alri
Nefefe, Tshifhiwa
van Schalkwyk, Antoinette
Kara, Pravesh
Heath, Livio
Williamson, Anna-Lise
The Development of Dual Vaccines against Lumpy Skin Disease (LSD) and Bovine Ephemeral Fever (BEF)
title The Development of Dual Vaccines against Lumpy Skin Disease (LSD) and Bovine Ephemeral Fever (BEF)
title_full The Development of Dual Vaccines against Lumpy Skin Disease (LSD) and Bovine Ephemeral Fever (BEF)
title_fullStr The Development of Dual Vaccines against Lumpy Skin Disease (LSD) and Bovine Ephemeral Fever (BEF)
title_full_unstemmed The Development of Dual Vaccines against Lumpy Skin Disease (LSD) and Bovine Ephemeral Fever (BEF)
title_short The Development of Dual Vaccines against Lumpy Skin Disease (LSD) and Bovine Ephemeral Fever (BEF)
title_sort development of dual vaccines against lumpy skin disease (lsd) and bovine ephemeral fever (bef)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8621795/
https://www.ncbi.nlm.nih.gov/pubmed/34835146
http://dx.doi.org/10.3390/vaccines9111215
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