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Gut Microbiome Profiling Uncovers a Lower Abundance of Butyricicoccus in Advanced Stages of Chronic Kidney Disease

Chronic kidney disease (CKD) is characterized by the accumulation of uremic toxins which exert deleterious effects on various organ systems. Several of these uremic toxins originate from the bacterial metabolization of aromatic amino acids in the colon. This study assessed whether the gut microbial...

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Autores principales: Gryp, Tessa, Faust, Karoline, Van Biesen, Wim, Huys, Geert R. B., Verbeke, Francis, Speeckaert, Marijn, Raes, Jeroen, Vaneechoutte, Mario, Joossens, Marie, Glorieux, Griet
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8621827/
https://www.ncbi.nlm.nih.gov/pubmed/34834470
http://dx.doi.org/10.3390/jpm11111118
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author Gryp, Tessa
Faust, Karoline
Van Biesen, Wim
Huys, Geert R. B.
Verbeke, Francis
Speeckaert, Marijn
Raes, Jeroen
Vaneechoutte, Mario
Joossens, Marie
Glorieux, Griet
author_facet Gryp, Tessa
Faust, Karoline
Van Biesen, Wim
Huys, Geert R. B.
Verbeke, Francis
Speeckaert, Marijn
Raes, Jeroen
Vaneechoutte, Mario
Joossens, Marie
Glorieux, Griet
author_sort Gryp, Tessa
collection PubMed
description Chronic kidney disease (CKD) is characterized by the accumulation of uremic toxins which exert deleterious effects on various organ systems. Several of these uremic toxins originate from the bacterial metabolization of aromatic amino acids in the colon. This study assessed whether the gut microbial composition varies among patients in different stages of CKD. Uremic metabolites were quantified by UPLC/fluorescence detection and microbial profiling by 16S rRNA amplicon sequencing. Gut microbial profiles of CKD patients were compared among stages 1–2, stage 3 and stages 4–5. Although a substantial inter-individual difference in abundance of the top 15 genera was observed, no significant difference was observed between groups. Bristol stool scale (BSS) correlated negatively with p-cresyl sulfate and hippuric acid levels, irrespective of the intake of laxatives. Butyricicoccus, a genus with butyrate-generating properties, was decreased in abundance in advanced stages of CKD compared to the earlier stages (p = 0.043). In conclusion, in this cross-sectional study no gradual differences in the gut microbial profile over the different stages of CKD were observed. However, the decrease in the abundance of Butyricicoccus genus with loss of kidney function stresses the need for more in-depth functional exploration of the gut microbiome in CKD patients not on dialysis.
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spelling pubmed-86218272021-11-27 Gut Microbiome Profiling Uncovers a Lower Abundance of Butyricicoccus in Advanced Stages of Chronic Kidney Disease Gryp, Tessa Faust, Karoline Van Biesen, Wim Huys, Geert R. B. Verbeke, Francis Speeckaert, Marijn Raes, Jeroen Vaneechoutte, Mario Joossens, Marie Glorieux, Griet J Pers Med Article Chronic kidney disease (CKD) is characterized by the accumulation of uremic toxins which exert deleterious effects on various organ systems. Several of these uremic toxins originate from the bacterial metabolization of aromatic amino acids in the colon. This study assessed whether the gut microbial composition varies among patients in different stages of CKD. Uremic metabolites were quantified by UPLC/fluorescence detection and microbial profiling by 16S rRNA amplicon sequencing. Gut microbial profiles of CKD patients were compared among stages 1–2, stage 3 and stages 4–5. Although a substantial inter-individual difference in abundance of the top 15 genera was observed, no significant difference was observed between groups. Bristol stool scale (BSS) correlated negatively with p-cresyl sulfate and hippuric acid levels, irrespective of the intake of laxatives. Butyricicoccus, a genus with butyrate-generating properties, was decreased in abundance in advanced stages of CKD compared to the earlier stages (p = 0.043). In conclusion, in this cross-sectional study no gradual differences in the gut microbial profile over the different stages of CKD were observed. However, the decrease in the abundance of Butyricicoccus genus with loss of kidney function stresses the need for more in-depth functional exploration of the gut microbiome in CKD patients not on dialysis. MDPI 2021-10-29 /pmc/articles/PMC8621827/ /pubmed/34834470 http://dx.doi.org/10.3390/jpm11111118 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gryp, Tessa
Faust, Karoline
Van Biesen, Wim
Huys, Geert R. B.
Verbeke, Francis
Speeckaert, Marijn
Raes, Jeroen
Vaneechoutte, Mario
Joossens, Marie
Glorieux, Griet
Gut Microbiome Profiling Uncovers a Lower Abundance of Butyricicoccus in Advanced Stages of Chronic Kidney Disease
title Gut Microbiome Profiling Uncovers a Lower Abundance of Butyricicoccus in Advanced Stages of Chronic Kidney Disease
title_full Gut Microbiome Profiling Uncovers a Lower Abundance of Butyricicoccus in Advanced Stages of Chronic Kidney Disease
title_fullStr Gut Microbiome Profiling Uncovers a Lower Abundance of Butyricicoccus in Advanced Stages of Chronic Kidney Disease
title_full_unstemmed Gut Microbiome Profiling Uncovers a Lower Abundance of Butyricicoccus in Advanced Stages of Chronic Kidney Disease
title_short Gut Microbiome Profiling Uncovers a Lower Abundance of Butyricicoccus in Advanced Stages of Chronic Kidney Disease
title_sort gut microbiome profiling uncovers a lower abundance of butyricicoccus in advanced stages of chronic kidney disease
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8621827/
https://www.ncbi.nlm.nih.gov/pubmed/34834470
http://dx.doi.org/10.3390/jpm11111118
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