Cargando…

Aggregatibacter actinomycetemcomitans and Aggregatibacter aphrophilus in a Kenyan Maasai Adolescent Population and Inhibition of Leukotoxic Activity by Herbal Plants Used as Part of Oral Hygiene Procedures

Background: A virulent genotype (JP2) of the periodonto-pathogen, Aggregatibacter actinomycetemcomitans (Aa), is widespread in North and West Africa, while its presence in East Africa has not been thoroughly investigated. This JP2 genotype is associated with periodontitis in adolescents and has a hi...

Descripción completa

Detalles Bibliográficos
Autores principales: Lindholm, Mark, Claesson, Rolf, Kemoli, Arthur, Mulli, Tonnie, Oscarsson, Jan, Haubek, Dorte, Johansson, Anders
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8621963/
https://www.ncbi.nlm.nih.gov/pubmed/34830683
http://dx.doi.org/10.3390/jcm10225402
_version_ 1784605581893435392
author Lindholm, Mark
Claesson, Rolf
Kemoli, Arthur
Mulli, Tonnie
Oscarsson, Jan
Haubek, Dorte
Johansson, Anders
author_facet Lindholm, Mark
Claesson, Rolf
Kemoli, Arthur
Mulli, Tonnie
Oscarsson, Jan
Haubek, Dorte
Johansson, Anders
author_sort Lindholm, Mark
collection PubMed
description Background: A virulent genotype (JP2) of the periodonto-pathogen, Aggregatibacter actinomycetemcomitans (Aa), is widespread in North and West Africa, while its presence in East Africa has not been thoroughly investigated. This JP2 genotype is associated with periodontitis in adolescents and has a high leukotoxicity. The aim of the study was to examine the prevalence of Aa and its JP2 genotype, the prevalence of the oral, commensal Aggregatibacter aphrophilus in a Maasai adolescent population, and the effect of herbal plants for inhibition of leukotoxicity. Methods: A total of 284 adolescents from Maasai Mara, Kenya, underwent an oral examination and microbial sampling. The presence of Aa and A. aphrophilus was analyzed by quantitative PCR and cultivation (the 58 samples collected at the last day of field study). The collected Aa strains were characterized and leukotoxin promoter typed. Additionally, herbal plants commonly used for oral hygiene were assessed for the inhibition of leukotoxicity. Results and Conclusions: The prevalence of Aa in stimulated whole saliva was high (71.8%), with the JP2 genotype detected in one individual, and A. aphrophilus in 99% of the sampled individuals. The commonly used herbal plant, Warburgia ugandensis, inactivated Aa leukotoxicity. The Aa virulence might be reduced through use of W. ugandensis and the high levels of A. aphrophilus.
format Online
Article
Text
id pubmed-8621963
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-86219632021-11-27 Aggregatibacter actinomycetemcomitans and Aggregatibacter aphrophilus in a Kenyan Maasai Adolescent Population and Inhibition of Leukotoxic Activity by Herbal Plants Used as Part of Oral Hygiene Procedures Lindholm, Mark Claesson, Rolf Kemoli, Arthur Mulli, Tonnie Oscarsson, Jan Haubek, Dorte Johansson, Anders J Clin Med Article Background: A virulent genotype (JP2) of the periodonto-pathogen, Aggregatibacter actinomycetemcomitans (Aa), is widespread in North and West Africa, while its presence in East Africa has not been thoroughly investigated. This JP2 genotype is associated with periodontitis in adolescents and has a high leukotoxicity. The aim of the study was to examine the prevalence of Aa and its JP2 genotype, the prevalence of the oral, commensal Aggregatibacter aphrophilus in a Maasai adolescent population, and the effect of herbal plants for inhibition of leukotoxicity. Methods: A total of 284 adolescents from Maasai Mara, Kenya, underwent an oral examination and microbial sampling. The presence of Aa and A. aphrophilus was analyzed by quantitative PCR and cultivation (the 58 samples collected at the last day of field study). The collected Aa strains were characterized and leukotoxin promoter typed. Additionally, herbal plants commonly used for oral hygiene were assessed for the inhibition of leukotoxicity. Results and Conclusions: The prevalence of Aa in stimulated whole saliva was high (71.8%), with the JP2 genotype detected in one individual, and A. aphrophilus in 99% of the sampled individuals. The commonly used herbal plant, Warburgia ugandensis, inactivated Aa leukotoxicity. The Aa virulence might be reduced through use of W. ugandensis and the high levels of A. aphrophilus. MDPI 2021-11-19 /pmc/articles/PMC8621963/ /pubmed/34830683 http://dx.doi.org/10.3390/jcm10225402 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lindholm, Mark
Claesson, Rolf
Kemoli, Arthur
Mulli, Tonnie
Oscarsson, Jan
Haubek, Dorte
Johansson, Anders
Aggregatibacter actinomycetemcomitans and Aggregatibacter aphrophilus in a Kenyan Maasai Adolescent Population and Inhibition of Leukotoxic Activity by Herbal Plants Used as Part of Oral Hygiene Procedures
title Aggregatibacter actinomycetemcomitans and Aggregatibacter aphrophilus in a Kenyan Maasai Adolescent Population and Inhibition of Leukotoxic Activity by Herbal Plants Used as Part of Oral Hygiene Procedures
title_full Aggregatibacter actinomycetemcomitans and Aggregatibacter aphrophilus in a Kenyan Maasai Adolescent Population and Inhibition of Leukotoxic Activity by Herbal Plants Used as Part of Oral Hygiene Procedures
title_fullStr Aggregatibacter actinomycetemcomitans and Aggregatibacter aphrophilus in a Kenyan Maasai Adolescent Population and Inhibition of Leukotoxic Activity by Herbal Plants Used as Part of Oral Hygiene Procedures
title_full_unstemmed Aggregatibacter actinomycetemcomitans and Aggregatibacter aphrophilus in a Kenyan Maasai Adolescent Population and Inhibition of Leukotoxic Activity by Herbal Plants Used as Part of Oral Hygiene Procedures
title_short Aggregatibacter actinomycetemcomitans and Aggregatibacter aphrophilus in a Kenyan Maasai Adolescent Population and Inhibition of Leukotoxic Activity by Herbal Plants Used as Part of Oral Hygiene Procedures
title_sort aggregatibacter actinomycetemcomitans and aggregatibacter aphrophilus in a kenyan maasai adolescent population and inhibition of leukotoxic activity by herbal plants used as part of oral hygiene procedures
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8621963/
https://www.ncbi.nlm.nih.gov/pubmed/34830683
http://dx.doi.org/10.3390/jcm10225402
work_keys_str_mv AT lindholmmark aggregatibacteractinomycetemcomitansandaggregatibacteraphrophilusinakenyanmaasaiadolescentpopulationandinhibitionofleukotoxicactivitybyherbalplantsusedaspartoforalhygieneprocedures
AT claessonrolf aggregatibacteractinomycetemcomitansandaggregatibacteraphrophilusinakenyanmaasaiadolescentpopulationandinhibitionofleukotoxicactivitybyherbalplantsusedaspartoforalhygieneprocedures
AT kemoliarthur aggregatibacteractinomycetemcomitansandaggregatibacteraphrophilusinakenyanmaasaiadolescentpopulationandinhibitionofleukotoxicactivitybyherbalplantsusedaspartoforalhygieneprocedures
AT mullitonnie aggregatibacteractinomycetemcomitansandaggregatibacteraphrophilusinakenyanmaasaiadolescentpopulationandinhibitionofleukotoxicactivitybyherbalplantsusedaspartoforalhygieneprocedures
AT oscarssonjan aggregatibacteractinomycetemcomitansandaggregatibacteraphrophilusinakenyanmaasaiadolescentpopulationandinhibitionofleukotoxicactivitybyherbalplantsusedaspartoforalhygieneprocedures
AT haubekdorte aggregatibacteractinomycetemcomitansandaggregatibacteraphrophilusinakenyanmaasaiadolescentpopulationandinhibitionofleukotoxicactivitybyherbalplantsusedaspartoforalhygieneprocedures
AT johanssonanders aggregatibacteractinomycetemcomitansandaggregatibacteraphrophilusinakenyanmaasaiadolescentpopulationandinhibitionofleukotoxicactivitybyherbalplantsusedaspartoforalhygieneprocedures