Cargando…

Feline Demodicosis Case Report—First Molecular Characterization of Demodex Mites in Romania

Cat demodicosis is uncommon to rare, and is caused by Demodex cati, Demodex gatoi and another unnamed species. The investigated patient was a mix-breed, 10-year-old feline with no dermatological history. Alopecia, erythema, minor erosions and ulcerations and crusts, associated with pruritus and self...

Descripción completa

Detalles Bibliográficos
Autores principales: Ilie, Marius Stelian, Imre, Mirela, Giubega, Simona, Luca, Iasmina, Florea, Tiana, Morariu, Sorin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8622192/
https://www.ncbi.nlm.nih.gov/pubmed/34832631
http://dx.doi.org/10.3390/pathogens10111474
_version_ 1784605636899635200
author Ilie, Marius Stelian
Imre, Mirela
Giubega, Simona
Luca, Iasmina
Florea, Tiana
Morariu, Sorin
author_facet Ilie, Marius Stelian
Imre, Mirela
Giubega, Simona
Luca, Iasmina
Florea, Tiana
Morariu, Sorin
author_sort Ilie, Marius Stelian
collection PubMed
description Cat demodicosis is uncommon to rare, and is caused by Demodex cati, Demodex gatoi and another unnamed species. The investigated patient was a mix-breed, 10-year-old feline with no dermatological history. Alopecia, erythema, minor erosions and ulcerations and crusts, associated with pruritus and self-trauma, were observed on the head. Dark, agglutinated cerumen was also present in the external ear canal. The agent causing the skin condition in the feline patient was identified as being a Demodex genus mite, based on the specific, morphological characteristics noticed upon the microscopic examination of deep skin scrapes. Biological samples were collected from the patient with to perform a PCR assay for clear species-determination and morphological assessment. PCR amplification of DNA extracted from the Demodex mites produced a single band of ~330 bp, indicating the presence of the D. cati species. The acaricidal treatment consisted of topical treatment using a fluralaner and moxidectin-based spot-on. Upon follow-up appointments, scheduled three times at a monthly interval, the patient failed to provide a positive result upon deep skin scrapes. The negative scrapes were also accompanied by the complete resolution of the existing lesions. In conclusion, this is the first molecular study to highlight the presence of Demodex cati within the feline population of Romania, and the fluralaner-moxidectin spot-on therapy has led to a complete recovery of the feline patient affected by feline demodicosis.
format Online
Article
Text
id pubmed-8622192
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-86221922021-11-27 Feline Demodicosis Case Report—First Molecular Characterization of Demodex Mites in Romania Ilie, Marius Stelian Imre, Mirela Giubega, Simona Luca, Iasmina Florea, Tiana Morariu, Sorin Pathogens Case Report Cat demodicosis is uncommon to rare, and is caused by Demodex cati, Demodex gatoi and another unnamed species. The investigated patient was a mix-breed, 10-year-old feline with no dermatological history. Alopecia, erythema, minor erosions and ulcerations and crusts, associated with pruritus and self-trauma, were observed on the head. Dark, agglutinated cerumen was also present in the external ear canal. The agent causing the skin condition in the feline patient was identified as being a Demodex genus mite, based on the specific, morphological characteristics noticed upon the microscopic examination of deep skin scrapes. Biological samples were collected from the patient with to perform a PCR assay for clear species-determination and morphological assessment. PCR amplification of DNA extracted from the Demodex mites produced a single band of ~330 bp, indicating the presence of the D. cati species. The acaricidal treatment consisted of topical treatment using a fluralaner and moxidectin-based spot-on. Upon follow-up appointments, scheduled three times at a monthly interval, the patient failed to provide a positive result upon deep skin scrapes. The negative scrapes were also accompanied by the complete resolution of the existing lesions. In conclusion, this is the first molecular study to highlight the presence of Demodex cati within the feline population of Romania, and the fluralaner-moxidectin spot-on therapy has led to a complete recovery of the feline patient affected by feline demodicosis. MDPI 2021-11-12 /pmc/articles/PMC8622192/ /pubmed/34832631 http://dx.doi.org/10.3390/pathogens10111474 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Case Report
Ilie, Marius Stelian
Imre, Mirela
Giubega, Simona
Luca, Iasmina
Florea, Tiana
Morariu, Sorin
Feline Demodicosis Case Report—First Molecular Characterization of Demodex Mites in Romania
title Feline Demodicosis Case Report—First Molecular Characterization of Demodex Mites in Romania
title_full Feline Demodicosis Case Report—First Molecular Characterization of Demodex Mites in Romania
title_fullStr Feline Demodicosis Case Report—First Molecular Characterization of Demodex Mites in Romania
title_full_unstemmed Feline Demodicosis Case Report—First Molecular Characterization of Demodex Mites in Romania
title_short Feline Demodicosis Case Report—First Molecular Characterization of Demodex Mites in Romania
title_sort feline demodicosis case report—first molecular characterization of demodex mites in romania
topic Case Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8622192/
https://www.ncbi.nlm.nih.gov/pubmed/34832631
http://dx.doi.org/10.3390/pathogens10111474
work_keys_str_mv AT iliemariusstelian felinedemodicosiscasereportfirstmolecularcharacterizationofdemodexmitesinromania
AT imremirela felinedemodicosiscasereportfirstmolecularcharacterizationofdemodexmitesinromania
AT giubegasimona felinedemodicosiscasereportfirstmolecularcharacterizationofdemodexmitesinromania
AT lucaiasmina felinedemodicosiscasereportfirstmolecularcharacterizationofdemodexmitesinromania
AT floreatiana felinedemodicosiscasereportfirstmolecularcharacterizationofdemodexmitesinromania
AT morariusorin felinedemodicosiscasereportfirstmolecularcharacterizationofdemodexmitesinromania