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Roles of OmpA in Type III Secretion System-Mediated Virulence of Enterohemorrhagic Escherichia coli

Outer membrane proteins are commonly produced by gram-negative bacteria, and they have diverse functions. A subgroup of proteins, which includes OmpA, OmpW and OmpX, is often involved in bacterial pathogenesis. Here we show that OmpA, rather than OmpW or OmpX, contributes to the virulence of enteroh...

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Autores principales: Hirakawa, Hidetada, Suzue, Kazutomo, Takita, Ayako, Tomita, Haruyoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8622347/
https://www.ncbi.nlm.nih.gov/pubmed/34832651
http://dx.doi.org/10.3390/pathogens10111496
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author Hirakawa, Hidetada
Suzue, Kazutomo
Takita, Ayako
Tomita, Haruyoshi
author_facet Hirakawa, Hidetada
Suzue, Kazutomo
Takita, Ayako
Tomita, Haruyoshi
author_sort Hirakawa, Hidetada
collection PubMed
description Outer membrane proteins are commonly produced by gram-negative bacteria, and they have diverse functions. A subgroup of proteins, which includes OmpA, OmpW and OmpX, is often involved in bacterial pathogenesis. Here we show that OmpA, rather than OmpW or OmpX, contributes to the virulence of enterohemorrhagic Escherichia coli (EHEC) through its type III secretion system (T3SS). Deletion of ompA decreased secretion of the T3SS proteins EspA and EspB; however, the expression level of the LEE genes that encode a set of T3SS proteins did not decrease. The ompA mutant had less abilities to form A/E lesions in host epithelial cells and lyse human red blood cells than the parent strain. Moreover, the virulence of an ompA mutant of Citrobacter rodentium (traditionally used to estimate T3SS-associated virulence in mice) was attenuated. Mice infected with the ompA mutant survived longer than those infected with the parent strain. Furthermore, mice infected with ompA developed symptoms of diarrhea more slowly than mice infected with the parent strain. Altogether, these results suggest that OmpA sustains the activity of the T3SS and is required for optimal virulence in EHEC. This work expands the roles of outer membrane proteins in bacterial pathogenesis.
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spelling pubmed-86223472021-11-27 Roles of OmpA in Type III Secretion System-Mediated Virulence of Enterohemorrhagic Escherichia coli Hirakawa, Hidetada Suzue, Kazutomo Takita, Ayako Tomita, Haruyoshi Pathogens Article Outer membrane proteins are commonly produced by gram-negative bacteria, and they have diverse functions. A subgroup of proteins, which includes OmpA, OmpW and OmpX, is often involved in bacterial pathogenesis. Here we show that OmpA, rather than OmpW or OmpX, contributes to the virulence of enterohemorrhagic Escherichia coli (EHEC) through its type III secretion system (T3SS). Deletion of ompA decreased secretion of the T3SS proteins EspA and EspB; however, the expression level of the LEE genes that encode a set of T3SS proteins did not decrease. The ompA mutant had less abilities to form A/E lesions in host epithelial cells and lyse human red blood cells than the parent strain. Moreover, the virulence of an ompA mutant of Citrobacter rodentium (traditionally used to estimate T3SS-associated virulence in mice) was attenuated. Mice infected with the ompA mutant survived longer than those infected with the parent strain. Furthermore, mice infected with ompA developed symptoms of diarrhea more slowly than mice infected with the parent strain. Altogether, these results suggest that OmpA sustains the activity of the T3SS and is required for optimal virulence in EHEC. This work expands the roles of outer membrane proteins in bacterial pathogenesis. MDPI 2021-11-17 /pmc/articles/PMC8622347/ /pubmed/34832651 http://dx.doi.org/10.3390/pathogens10111496 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hirakawa, Hidetada
Suzue, Kazutomo
Takita, Ayako
Tomita, Haruyoshi
Roles of OmpA in Type III Secretion System-Mediated Virulence of Enterohemorrhagic Escherichia coli
title Roles of OmpA in Type III Secretion System-Mediated Virulence of Enterohemorrhagic Escherichia coli
title_full Roles of OmpA in Type III Secretion System-Mediated Virulence of Enterohemorrhagic Escherichia coli
title_fullStr Roles of OmpA in Type III Secretion System-Mediated Virulence of Enterohemorrhagic Escherichia coli
title_full_unstemmed Roles of OmpA in Type III Secretion System-Mediated Virulence of Enterohemorrhagic Escherichia coli
title_short Roles of OmpA in Type III Secretion System-Mediated Virulence of Enterohemorrhagic Escherichia coli
title_sort roles of ompa in type iii secretion system-mediated virulence of enterohemorrhagic escherichia coli
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8622347/
https://www.ncbi.nlm.nih.gov/pubmed/34832651
http://dx.doi.org/10.3390/pathogens10111496
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