Cargando…

Synbiotics Alleviate Hepatic Damage, Intestinal Injury and Muscular Beclin-1 Elevation in Rats after Chronic Ethanol Administration

The purpose of this study was to investigate the beneficial effects of synbiotics on liver damage, intestinal health, and muscle loss, and their relevance in rats with chronic ethanol feeding. Thirty Wistar rats fed with a control liquid diet were divided into control and synbiotics groups, which we...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Yi-Hsiu, Chiu, Wan-Chun, Xiao, Qian, Chen, Ya-Ling, Shirakawa, Hitoshi, Yang, Suh-Ching
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8622351/
https://www.ncbi.nlm.nih.gov/pubmed/34830430
http://dx.doi.org/10.3390/ijms222212547
_version_ 1784605673402662912
author Chen, Yi-Hsiu
Chiu, Wan-Chun
Xiao, Qian
Chen, Ya-Ling
Shirakawa, Hitoshi
Yang, Suh-Ching
author_facet Chen, Yi-Hsiu
Chiu, Wan-Chun
Xiao, Qian
Chen, Ya-Ling
Shirakawa, Hitoshi
Yang, Suh-Ching
author_sort Chen, Yi-Hsiu
collection PubMed
description The purpose of this study was to investigate the beneficial effects of synbiotics on liver damage, intestinal health, and muscle loss, and their relevance in rats with chronic ethanol feeding. Thirty Wistar rats fed with a control liquid diet were divided into control and synbiotics groups, which were respectively provided with water or synbiotics solution (1.5 g/kg body weight/day) for 2 weeks. From the 3rd to 8th week, the control group was divided into a C group (control liquid diet + water) and an E group (ethanol liquid diet + water). The synbiotics group was separated in to three groups, SC, ASE, and PSE. The SC group was given a control liquid diet with synbiotics solution; the ASE group was given ethanol liquid diet with synbiotics solution, and the PSE group was given ethanol liquid diet and water. As the results, the E group exhibited liver damage, including increased AST and ALT activities, hepatic fatty changes, and higher CYP2E1 expression. Intestinal mRNA expressions of occludin and claudin-1 were significantly decreased and the plasma endotoxin level was significantly higher in the E group. In muscles, beclin-1 was significantly increased in the E group. Compared to the E group, the PSE and ASE groups had lower plasma ALT activities, hepatic fatty changes, and CYP2E1 expression. The PSE and ASE groups had significantly higher intestinal occludin and claudin-1 mRNA expressions and lower muscular beclin-1 expression when compared to the E group. In conclusion, synbiotics supplementation might reduce protein expression of muscle protein degradation biomarkers such as beclin-1 in rats with chronic ethanol feeding, which is speculated to be linked to the improvement of intestinal tight junction and the reduction of liver damage.
format Online
Article
Text
id pubmed-8622351
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-86223512021-11-27 Synbiotics Alleviate Hepatic Damage, Intestinal Injury and Muscular Beclin-1 Elevation in Rats after Chronic Ethanol Administration Chen, Yi-Hsiu Chiu, Wan-Chun Xiao, Qian Chen, Ya-Ling Shirakawa, Hitoshi Yang, Suh-Ching Int J Mol Sci Article The purpose of this study was to investigate the beneficial effects of synbiotics on liver damage, intestinal health, and muscle loss, and their relevance in rats with chronic ethanol feeding. Thirty Wistar rats fed with a control liquid diet were divided into control and synbiotics groups, which were respectively provided with water or synbiotics solution (1.5 g/kg body weight/day) for 2 weeks. From the 3rd to 8th week, the control group was divided into a C group (control liquid diet + water) and an E group (ethanol liquid diet + water). The synbiotics group was separated in to three groups, SC, ASE, and PSE. The SC group was given a control liquid diet with synbiotics solution; the ASE group was given ethanol liquid diet with synbiotics solution, and the PSE group was given ethanol liquid diet and water. As the results, the E group exhibited liver damage, including increased AST and ALT activities, hepatic fatty changes, and higher CYP2E1 expression. Intestinal mRNA expressions of occludin and claudin-1 were significantly decreased and the plasma endotoxin level was significantly higher in the E group. In muscles, beclin-1 was significantly increased in the E group. Compared to the E group, the PSE and ASE groups had lower plasma ALT activities, hepatic fatty changes, and CYP2E1 expression. The PSE and ASE groups had significantly higher intestinal occludin and claudin-1 mRNA expressions and lower muscular beclin-1 expression when compared to the E group. In conclusion, synbiotics supplementation might reduce protein expression of muscle protein degradation biomarkers such as beclin-1 in rats with chronic ethanol feeding, which is speculated to be linked to the improvement of intestinal tight junction and the reduction of liver damage. MDPI 2021-11-21 /pmc/articles/PMC8622351/ /pubmed/34830430 http://dx.doi.org/10.3390/ijms222212547 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chen, Yi-Hsiu
Chiu, Wan-Chun
Xiao, Qian
Chen, Ya-Ling
Shirakawa, Hitoshi
Yang, Suh-Ching
Synbiotics Alleviate Hepatic Damage, Intestinal Injury and Muscular Beclin-1 Elevation in Rats after Chronic Ethanol Administration
title Synbiotics Alleviate Hepatic Damage, Intestinal Injury and Muscular Beclin-1 Elevation in Rats after Chronic Ethanol Administration
title_full Synbiotics Alleviate Hepatic Damage, Intestinal Injury and Muscular Beclin-1 Elevation in Rats after Chronic Ethanol Administration
title_fullStr Synbiotics Alleviate Hepatic Damage, Intestinal Injury and Muscular Beclin-1 Elevation in Rats after Chronic Ethanol Administration
title_full_unstemmed Synbiotics Alleviate Hepatic Damage, Intestinal Injury and Muscular Beclin-1 Elevation in Rats after Chronic Ethanol Administration
title_short Synbiotics Alleviate Hepatic Damage, Intestinal Injury and Muscular Beclin-1 Elevation in Rats after Chronic Ethanol Administration
title_sort synbiotics alleviate hepatic damage, intestinal injury and muscular beclin-1 elevation in rats after chronic ethanol administration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8622351/
https://www.ncbi.nlm.nih.gov/pubmed/34830430
http://dx.doi.org/10.3390/ijms222212547
work_keys_str_mv AT chenyihsiu synbioticsalleviatehepaticdamageintestinalinjuryandmuscularbeclin1elevationinratsafterchronicethanoladministration
AT chiuwanchun synbioticsalleviatehepaticdamageintestinalinjuryandmuscularbeclin1elevationinratsafterchronicethanoladministration
AT xiaoqian synbioticsalleviatehepaticdamageintestinalinjuryandmuscularbeclin1elevationinratsafterchronicethanoladministration
AT chenyaling synbioticsalleviatehepaticdamageintestinalinjuryandmuscularbeclin1elevationinratsafterchronicethanoladministration
AT shirakawahitoshi synbioticsalleviatehepaticdamageintestinalinjuryandmuscularbeclin1elevationinratsafterchronicethanoladministration
AT yangsuhching synbioticsalleviatehepaticdamageintestinalinjuryandmuscularbeclin1elevationinratsafterchronicethanoladministration