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Ceftriaxone Mediated Synthesized Gold Nanoparticles: A Nano-Therapeutic Tool to Target Bacterial Resistance

Ceftriaxone has been a part of therapeutic regime for combating some of the most aggressive bacterial infections in the last few decades. However, increasing bacterial resistance towards ceftriaxone and other third generation cephalosporin antibiotics has raised serious clinical concerns especially...

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Autores principales: Alshammari, Farhan, Alshammari, Bushra, Moin, Afrasim, Alamri, Abdulwahab, Al Hagbani, Turki, Alobaida, Ahmed, Baker, Abu, Khan, Salman, Rizvi, Syed Mohd Danish
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8622407/
https://www.ncbi.nlm.nih.gov/pubmed/34834310
http://dx.doi.org/10.3390/pharmaceutics13111896
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author Alshammari, Farhan
Alshammari, Bushra
Moin, Afrasim
Alamri, Abdulwahab
Al Hagbani, Turki
Alobaida, Ahmed
Baker, Abu
Khan, Salman
Rizvi, Syed Mohd Danish
author_facet Alshammari, Farhan
Alshammari, Bushra
Moin, Afrasim
Alamri, Abdulwahab
Al Hagbani, Turki
Alobaida, Ahmed
Baker, Abu
Khan, Salman
Rizvi, Syed Mohd Danish
author_sort Alshammari, Farhan
collection PubMed
description Ceftriaxone has been a part of therapeutic regime for combating some of the most aggressive bacterial infections in the last few decades. However, increasing bacterial resistance towards ceftriaxone and other third generation cephalosporin antibiotics has raised serious clinical concerns especially due to their misuse in the COVID-19 era. Advancement in nanotechnology has converted nano-therapeutic vision into a plausible reality with better targeting and reduced drug consumption. Thus, in the present study, gold nanoparticles (GNPs) were synthesized by using ceftriaxone antibiotic that acts as a reducing as well as capping agent. Ceftriaxone-loaded GNPs (CGNPs) were initially characterized by UV-visible spectroscopy, DLS, Zeta potential, Electron microscopy and FT-IR. However, a TEM micrograph showed a uniform size of 21 ± 1 nm for the synthesized CGNPs. Further, both (CGNPs) and pure ceftriaxone were examined for their efficacy against Escherichia coli, Staphylococcus aureus, Salmonella abony and Klebsiella pneumoniae. CGNPs showed MIC(50) as 1.39, 1.6, 1.1 and 0.9 µg/mL against E. coli, S. aureus, S. abony and K. pneumoniae, respectively. Interestingly, CGNPs showed two times better efficacy when compared with pure ceftriaxone against the tested bacterial strains. Restoring the potential of unresponsive or less efficient ceftriaxone via gold nanoformulations is the most alluring concept of the whole study. Moreover, applicability of the findings from bench to bedside needs further validation.
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spelling pubmed-86224072021-11-27 Ceftriaxone Mediated Synthesized Gold Nanoparticles: A Nano-Therapeutic Tool to Target Bacterial Resistance Alshammari, Farhan Alshammari, Bushra Moin, Afrasim Alamri, Abdulwahab Al Hagbani, Turki Alobaida, Ahmed Baker, Abu Khan, Salman Rizvi, Syed Mohd Danish Pharmaceutics Article Ceftriaxone has been a part of therapeutic regime for combating some of the most aggressive bacterial infections in the last few decades. However, increasing bacterial resistance towards ceftriaxone and other third generation cephalosporin antibiotics has raised serious clinical concerns especially due to their misuse in the COVID-19 era. Advancement in nanotechnology has converted nano-therapeutic vision into a plausible reality with better targeting and reduced drug consumption. Thus, in the present study, gold nanoparticles (GNPs) were synthesized by using ceftriaxone antibiotic that acts as a reducing as well as capping agent. Ceftriaxone-loaded GNPs (CGNPs) were initially characterized by UV-visible spectroscopy, DLS, Zeta potential, Electron microscopy and FT-IR. However, a TEM micrograph showed a uniform size of 21 ± 1 nm for the synthesized CGNPs. Further, both (CGNPs) and pure ceftriaxone were examined for their efficacy against Escherichia coli, Staphylococcus aureus, Salmonella abony and Klebsiella pneumoniae. CGNPs showed MIC(50) as 1.39, 1.6, 1.1 and 0.9 µg/mL against E. coli, S. aureus, S. abony and K. pneumoniae, respectively. Interestingly, CGNPs showed two times better efficacy when compared with pure ceftriaxone against the tested bacterial strains. Restoring the potential of unresponsive or less efficient ceftriaxone via gold nanoformulations is the most alluring concept of the whole study. Moreover, applicability of the findings from bench to bedside needs further validation. MDPI 2021-11-08 /pmc/articles/PMC8622407/ /pubmed/34834310 http://dx.doi.org/10.3390/pharmaceutics13111896 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Alshammari, Farhan
Alshammari, Bushra
Moin, Afrasim
Alamri, Abdulwahab
Al Hagbani, Turki
Alobaida, Ahmed
Baker, Abu
Khan, Salman
Rizvi, Syed Mohd Danish
Ceftriaxone Mediated Synthesized Gold Nanoparticles: A Nano-Therapeutic Tool to Target Bacterial Resistance
title Ceftriaxone Mediated Synthesized Gold Nanoparticles: A Nano-Therapeutic Tool to Target Bacterial Resistance
title_full Ceftriaxone Mediated Synthesized Gold Nanoparticles: A Nano-Therapeutic Tool to Target Bacterial Resistance
title_fullStr Ceftriaxone Mediated Synthesized Gold Nanoparticles: A Nano-Therapeutic Tool to Target Bacterial Resistance
title_full_unstemmed Ceftriaxone Mediated Synthesized Gold Nanoparticles: A Nano-Therapeutic Tool to Target Bacterial Resistance
title_short Ceftriaxone Mediated Synthesized Gold Nanoparticles: A Nano-Therapeutic Tool to Target Bacterial Resistance
title_sort ceftriaxone mediated synthesized gold nanoparticles: a nano-therapeutic tool to target bacterial resistance
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8622407/
https://www.ncbi.nlm.nih.gov/pubmed/34834310
http://dx.doi.org/10.3390/pharmaceutics13111896
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