Cargando…
Metabolomic Characterization of Cerebrospinal Fluid from Intracranial Bacterial Infection Pediatric Patients: A Pilot Study
Intracranial bacterial infection remains a major cause of morbidity and mortality in neurosurgical cases. Metabolomic profiling of cerebrospinal fluid (CSF) holds great promise to gain insights into the pathogenesis of central neural system (CNS) bacterial infections. In this pilot study, we analyze...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8622478/ https://www.ncbi.nlm.nih.gov/pubmed/34833963 http://dx.doi.org/10.3390/molecules26226871 |
_version_ | 1784605703064780800 |
---|---|
author | Wang, Yiwen Liu, Yu Chen, Ruoping Qiao, Liang |
author_facet | Wang, Yiwen Liu, Yu Chen, Ruoping Qiao, Liang |
author_sort | Wang, Yiwen |
collection | PubMed |
description | Intracranial bacterial infection remains a major cause of morbidity and mortality in neurosurgical cases. Metabolomic profiling of cerebrospinal fluid (CSF) holds great promise to gain insights into the pathogenesis of central neural system (CNS) bacterial infections. In this pilot study, we analyzed the metabolites in CSF of CNS infection patients and controls in a pseudo-targeted manner, aiming at elucidating the metabolic dysregulation in response to postoperative intracranial bacterial infection of pediatric cases. Untargeted analysis uncovered 597 metabolites, and screened out 206 differential metabolites in case of infection. Targeted verification and pathway analysis filtered out the glycolysis, amino acids metabolism and purine metabolism pathways as potential pathological pathways. These perturbed pathways are involved in the infection-induced oxidative stress and immune response. Characterization of the infection-induced metabolic changes can provide robust biomarkers of CNS bacterial infection for clinical diagnosis, novel pathways for pathological investigation, and new targets for treatment. |
format | Online Article Text |
id | pubmed-8622478 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-86224782021-11-27 Metabolomic Characterization of Cerebrospinal Fluid from Intracranial Bacterial Infection Pediatric Patients: A Pilot Study Wang, Yiwen Liu, Yu Chen, Ruoping Qiao, Liang Molecules Article Intracranial bacterial infection remains a major cause of morbidity and mortality in neurosurgical cases. Metabolomic profiling of cerebrospinal fluid (CSF) holds great promise to gain insights into the pathogenesis of central neural system (CNS) bacterial infections. In this pilot study, we analyzed the metabolites in CSF of CNS infection patients and controls in a pseudo-targeted manner, aiming at elucidating the metabolic dysregulation in response to postoperative intracranial bacterial infection of pediatric cases. Untargeted analysis uncovered 597 metabolites, and screened out 206 differential metabolites in case of infection. Targeted verification and pathway analysis filtered out the glycolysis, amino acids metabolism and purine metabolism pathways as potential pathological pathways. These perturbed pathways are involved in the infection-induced oxidative stress and immune response. Characterization of the infection-induced metabolic changes can provide robust biomarkers of CNS bacterial infection for clinical diagnosis, novel pathways for pathological investigation, and new targets for treatment. MDPI 2021-11-15 /pmc/articles/PMC8622478/ /pubmed/34833963 http://dx.doi.org/10.3390/molecules26226871 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wang, Yiwen Liu, Yu Chen, Ruoping Qiao, Liang Metabolomic Characterization of Cerebrospinal Fluid from Intracranial Bacterial Infection Pediatric Patients: A Pilot Study |
title | Metabolomic Characterization of Cerebrospinal Fluid from Intracranial Bacterial Infection Pediatric Patients: A Pilot Study |
title_full | Metabolomic Characterization of Cerebrospinal Fluid from Intracranial Bacterial Infection Pediatric Patients: A Pilot Study |
title_fullStr | Metabolomic Characterization of Cerebrospinal Fluid from Intracranial Bacterial Infection Pediatric Patients: A Pilot Study |
title_full_unstemmed | Metabolomic Characterization of Cerebrospinal Fluid from Intracranial Bacterial Infection Pediatric Patients: A Pilot Study |
title_short | Metabolomic Characterization of Cerebrospinal Fluid from Intracranial Bacterial Infection Pediatric Patients: A Pilot Study |
title_sort | metabolomic characterization of cerebrospinal fluid from intracranial bacterial infection pediatric patients: a pilot study |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8622478/ https://www.ncbi.nlm.nih.gov/pubmed/34833963 http://dx.doi.org/10.3390/molecules26226871 |
work_keys_str_mv | AT wangyiwen metabolomiccharacterizationofcerebrospinalfluidfromintracranialbacterialinfectionpediatricpatientsapilotstudy AT liuyu metabolomiccharacterizationofcerebrospinalfluidfromintracranialbacterialinfectionpediatricpatientsapilotstudy AT chenruoping metabolomiccharacterizationofcerebrospinalfluidfromintracranialbacterialinfectionpediatricpatientsapilotstudy AT qiaoliang metabolomiccharacterizationofcerebrospinalfluidfromintracranialbacterialinfectionpediatricpatientsapilotstudy |