Cargando…

Development of Various Leishmania (Sauroleishmania) tarentolae Strains in Three Phlebotomus Species

Leishmania (Sauroleishmania) tarentolae is transmitted by reptile-biting sand flies of the genus Sergentomyia, but the role of Phlebotomus sand flies in circulation of this parasite is unknown. Here, we compared the development of L. (S.) tarentolae strains in three Phlebotomus species: P. papatasi,...

Descripción completa

Detalles Bibliográficos
Autores principales: Ticha, Lucie, Kykalova, Barbora, Sadlova, Jovana, Gramiccia, Marina, Gradoni, Luigi, Volf, Petr
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8622532/
https://www.ncbi.nlm.nih.gov/pubmed/34835382
http://dx.doi.org/10.3390/microorganisms9112256
_version_ 1784605715679150080
author Ticha, Lucie
Kykalova, Barbora
Sadlova, Jovana
Gramiccia, Marina
Gradoni, Luigi
Volf, Petr
author_facet Ticha, Lucie
Kykalova, Barbora
Sadlova, Jovana
Gramiccia, Marina
Gradoni, Luigi
Volf, Petr
author_sort Ticha, Lucie
collection PubMed
description Leishmania (Sauroleishmania) tarentolae is transmitted by reptile-biting sand flies of the genus Sergentomyia, but the role of Phlebotomus sand flies in circulation of this parasite is unknown. Here, we compared the development of L. (S.) tarentolae strains in three Phlebotomus species: P. papatasi, P. sergenti, and P. perniciosus. Laboratory-bred sand flies were membrane-fed on blood with parasite suspension and dissected on days 1 and 7 post blood meal. Parasites were measured on Giemsa-stained gut smears and five morphological forms were distinguished. In all parasite-vector combinations, promastigotes were found in Malpighian tubules, often in high numbers, which suggests that this tissue is a typical location for L. (S.) tarentolae development in sand flies. All three studied strains colonized the hindgut, but also migrated anteriorly to both parts of the midgut and colonized the stomodeal valve. Significant differences were demonstrated between sand fly species: highest infection rates, high parasite loads, and the most frequent anterior migration with colonization of the stomodeal valve were found in P. perniciosus, while all these parameters were lowest in P. sergenti. In conclusion, the peripylarian type of development was demonstrated for three L. (S.) tarentolae strains in three Phlebotomus sand flies. We suggest paying more attention to Phlebotomus species, particularly P. perniciosus and P. papatasi, as potential secondary vectors of Sauroleishmania.
format Online
Article
Text
id pubmed-8622532
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-86225322021-11-27 Development of Various Leishmania (Sauroleishmania) tarentolae Strains in Three Phlebotomus Species Ticha, Lucie Kykalova, Barbora Sadlova, Jovana Gramiccia, Marina Gradoni, Luigi Volf, Petr Microorganisms Article Leishmania (Sauroleishmania) tarentolae is transmitted by reptile-biting sand flies of the genus Sergentomyia, but the role of Phlebotomus sand flies in circulation of this parasite is unknown. Here, we compared the development of L. (S.) tarentolae strains in three Phlebotomus species: P. papatasi, P. sergenti, and P. perniciosus. Laboratory-bred sand flies were membrane-fed on blood with parasite suspension and dissected on days 1 and 7 post blood meal. Parasites were measured on Giemsa-stained gut smears and five morphological forms were distinguished. In all parasite-vector combinations, promastigotes were found in Malpighian tubules, often in high numbers, which suggests that this tissue is a typical location for L. (S.) tarentolae development in sand flies. All three studied strains colonized the hindgut, but also migrated anteriorly to both parts of the midgut and colonized the stomodeal valve. Significant differences were demonstrated between sand fly species: highest infection rates, high parasite loads, and the most frequent anterior migration with colonization of the stomodeal valve were found in P. perniciosus, while all these parameters were lowest in P. sergenti. In conclusion, the peripylarian type of development was demonstrated for three L. (S.) tarentolae strains in three Phlebotomus sand flies. We suggest paying more attention to Phlebotomus species, particularly P. perniciosus and P. papatasi, as potential secondary vectors of Sauroleishmania. MDPI 2021-10-29 /pmc/articles/PMC8622532/ /pubmed/34835382 http://dx.doi.org/10.3390/microorganisms9112256 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ticha, Lucie
Kykalova, Barbora
Sadlova, Jovana
Gramiccia, Marina
Gradoni, Luigi
Volf, Petr
Development of Various Leishmania (Sauroleishmania) tarentolae Strains in Three Phlebotomus Species
title Development of Various Leishmania (Sauroleishmania) tarentolae Strains in Three Phlebotomus Species
title_full Development of Various Leishmania (Sauroleishmania) tarentolae Strains in Three Phlebotomus Species
title_fullStr Development of Various Leishmania (Sauroleishmania) tarentolae Strains in Three Phlebotomus Species
title_full_unstemmed Development of Various Leishmania (Sauroleishmania) tarentolae Strains in Three Phlebotomus Species
title_short Development of Various Leishmania (Sauroleishmania) tarentolae Strains in Three Phlebotomus Species
title_sort development of various leishmania (sauroleishmania) tarentolae strains in three phlebotomus species
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8622532/
https://www.ncbi.nlm.nih.gov/pubmed/34835382
http://dx.doi.org/10.3390/microorganisms9112256
work_keys_str_mv AT tichalucie developmentofvariousleishmaniasauroleishmaniatarentolaestrainsinthreephlebotomusspecies
AT kykalovabarbora developmentofvariousleishmaniasauroleishmaniatarentolaestrainsinthreephlebotomusspecies
AT sadlovajovana developmentofvariousleishmaniasauroleishmaniatarentolaestrainsinthreephlebotomusspecies
AT gramicciamarina developmentofvariousleishmaniasauroleishmaniatarentolaestrainsinthreephlebotomusspecies
AT gradoniluigi developmentofvariousleishmaniasauroleishmaniatarentolaestrainsinthreephlebotomusspecies
AT volfpetr developmentofvariousleishmaniasauroleishmaniatarentolaestrainsinthreephlebotomusspecies