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Transmission of Escherichia coli from Manure to Root Zones of Field-Grown Lettuce and Leek Plants

Pathogenic Escherichia coli strains are responsible for food-borne disease outbreaks upon consumption of fresh vegetables and fruits. The aim of this study was to establish the transmission route of E. coli strain 0611, as proxy for human pathogenic E. coli, via manure, soil and plant root zones to...

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Autores principales: van Overbeek, Leo, Duhamel, Marie, Aanstoot, Stefan, van der Plas, Carin Lombaers, Nijhuis, Els, Poleij, Leo, Russ, Lina, van der Zouwen, Patricia, Andreo-Jimenez, Beatriz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8622635/
https://www.ncbi.nlm.nih.gov/pubmed/34835415
http://dx.doi.org/10.3390/microorganisms9112289
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author van Overbeek, Leo
Duhamel, Marie
Aanstoot, Stefan
van der Plas, Carin Lombaers
Nijhuis, Els
Poleij, Leo
Russ, Lina
van der Zouwen, Patricia
Andreo-Jimenez, Beatriz
author_facet van Overbeek, Leo
Duhamel, Marie
Aanstoot, Stefan
van der Plas, Carin Lombaers
Nijhuis, Els
Poleij, Leo
Russ, Lina
van der Zouwen, Patricia
Andreo-Jimenez, Beatriz
author_sort van Overbeek, Leo
collection PubMed
description Pathogenic Escherichia coli strains are responsible for food-borne disease outbreaks upon consumption of fresh vegetables and fruits. The aim of this study was to establish the transmission route of E. coli strain 0611, as proxy for human pathogenic E. coli, via manure, soil and plant root zones to the above-soil plant compartments. The ecological behavior of the introduced strain was established by making use of a combination of cultivation-based and molecular targeted and untargeted approaches. Strain 0611 CFUs and specific molecular targets were detected in the root zones of lettuce and leek plants, even up to 272 days after planting in the case of leek plants. However, no strain 0611 colonies were detected in leek leaves, and only in one occasion a single colony was found in lettuce leaves. Therefore, it was concluded that transmission of E. coli via manure is not the principal contamination route to the edible parts of both plant species grown under field conditions in this study. Strain 0611 was shown to accumulate in root zones of both species and metagenomic reads of this strain were retrieved from the lettuce rhizosphere soil metagenome library at a level of Log 4.11 CFU per g dry soil.
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spelling pubmed-86226352021-11-27 Transmission of Escherichia coli from Manure to Root Zones of Field-Grown Lettuce and Leek Plants van Overbeek, Leo Duhamel, Marie Aanstoot, Stefan van der Plas, Carin Lombaers Nijhuis, Els Poleij, Leo Russ, Lina van der Zouwen, Patricia Andreo-Jimenez, Beatriz Microorganisms Article Pathogenic Escherichia coli strains are responsible for food-borne disease outbreaks upon consumption of fresh vegetables and fruits. The aim of this study was to establish the transmission route of E. coli strain 0611, as proxy for human pathogenic E. coli, via manure, soil and plant root zones to the above-soil plant compartments. The ecological behavior of the introduced strain was established by making use of a combination of cultivation-based and molecular targeted and untargeted approaches. Strain 0611 CFUs and specific molecular targets were detected in the root zones of lettuce and leek plants, even up to 272 days after planting in the case of leek plants. However, no strain 0611 colonies were detected in leek leaves, and only in one occasion a single colony was found in lettuce leaves. Therefore, it was concluded that transmission of E. coli via manure is not the principal contamination route to the edible parts of both plant species grown under field conditions in this study. Strain 0611 was shown to accumulate in root zones of both species and metagenomic reads of this strain were retrieved from the lettuce rhizosphere soil metagenome library at a level of Log 4.11 CFU per g dry soil. MDPI 2021-11-03 /pmc/articles/PMC8622635/ /pubmed/34835415 http://dx.doi.org/10.3390/microorganisms9112289 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
van Overbeek, Leo
Duhamel, Marie
Aanstoot, Stefan
van der Plas, Carin Lombaers
Nijhuis, Els
Poleij, Leo
Russ, Lina
van der Zouwen, Patricia
Andreo-Jimenez, Beatriz
Transmission of Escherichia coli from Manure to Root Zones of Field-Grown Lettuce and Leek Plants
title Transmission of Escherichia coli from Manure to Root Zones of Field-Grown Lettuce and Leek Plants
title_full Transmission of Escherichia coli from Manure to Root Zones of Field-Grown Lettuce and Leek Plants
title_fullStr Transmission of Escherichia coli from Manure to Root Zones of Field-Grown Lettuce and Leek Plants
title_full_unstemmed Transmission of Escherichia coli from Manure to Root Zones of Field-Grown Lettuce and Leek Plants
title_short Transmission of Escherichia coli from Manure to Root Zones of Field-Grown Lettuce and Leek Plants
title_sort transmission of escherichia coli from manure to root zones of field-grown lettuce and leek plants
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8622635/
https://www.ncbi.nlm.nih.gov/pubmed/34835415
http://dx.doi.org/10.3390/microorganisms9112289
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