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Parameters of Hemostasis in Sheep Implanted with Composite Scaffold Settled by Stimulated Mesenchymal Stem Cells—Evaluation of the Animal Model

Implantation of composite scaffolds could be potentially associated with the risk of hemostatic disturbances in a recipient. However, there is a lack of information on possible alterations in clotting mechanisms resulting from such a procedure. The aim of the present work was to investigate changes...

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Autores principales: Pliszczak-Król, Aleksandra, Kiełbowicz, Zdzisław, Król, Jarosław, Antończyk, Agnieszka, Gemra, Marianna, Skrzypczak, Piotr, Prządka, Przemysław, Zalewski, Dariusz, Bieżyński, Janusz, Nicpoń, Jakub
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8622787/
https://www.ncbi.nlm.nih.gov/pubmed/34832335
http://dx.doi.org/10.3390/ma14226934
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author Pliszczak-Król, Aleksandra
Kiełbowicz, Zdzisław
Król, Jarosław
Antończyk, Agnieszka
Gemra, Marianna
Skrzypczak, Piotr
Prządka, Przemysław
Zalewski, Dariusz
Bieżyński, Janusz
Nicpoń, Jakub
author_facet Pliszczak-Król, Aleksandra
Kiełbowicz, Zdzisław
Król, Jarosław
Antończyk, Agnieszka
Gemra, Marianna
Skrzypczak, Piotr
Prządka, Przemysław
Zalewski, Dariusz
Bieżyński, Janusz
Nicpoń, Jakub
author_sort Pliszczak-Król, Aleksandra
collection PubMed
description Implantation of composite scaffolds could be potentially associated with the risk of hemostatic disturbances in a recipient. However, there is a lack of information on possible alterations in clotting mechanisms resulting from such a procedure. The aim of the present work was to investigate changes in hemostatic parameters in sheep implanted with a scaffold composed of poly(ε-caprolactone) and hydroxyapatite and tricalcium phosphate (9:4.5:4.5), settled previously with mesenchymal stem cells stimulated by fibroblast growth factor-2 and bone morphogenetic protein-2. Nine Merino sheep were examined for 7 days, and measurements of clotting times (PT, aPTT), activities of antithrombin, protein C and clotting factors II-XII, and concentrations of fibrinogen and D-dimer were carried out before and 1 h, 24 h, 3 days and 7 days after scaffold implantation. The introduction of scaffold initially resulted in a slowdown of the clotting processes (most evident 24 h after surgery); PT and aPTT increased to 14.8 s and 33.9 s, respectively. From the third day onwards, most of these alterations began to return to normal values. The concentration of fibrinogen rose throughout the observation period (up to 8.4 g/L), mirroring the ongoing inflammatory reaction. However, no signals of significant disturbances in hemostatic processes were detected in the sheep tested.
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spelling pubmed-86227872021-11-27 Parameters of Hemostasis in Sheep Implanted with Composite Scaffold Settled by Stimulated Mesenchymal Stem Cells—Evaluation of the Animal Model Pliszczak-Król, Aleksandra Kiełbowicz, Zdzisław Król, Jarosław Antończyk, Agnieszka Gemra, Marianna Skrzypczak, Piotr Prządka, Przemysław Zalewski, Dariusz Bieżyński, Janusz Nicpoń, Jakub Materials (Basel) Article Implantation of composite scaffolds could be potentially associated with the risk of hemostatic disturbances in a recipient. However, there is a lack of information on possible alterations in clotting mechanisms resulting from such a procedure. The aim of the present work was to investigate changes in hemostatic parameters in sheep implanted with a scaffold composed of poly(ε-caprolactone) and hydroxyapatite and tricalcium phosphate (9:4.5:4.5), settled previously with mesenchymal stem cells stimulated by fibroblast growth factor-2 and bone morphogenetic protein-2. Nine Merino sheep were examined for 7 days, and measurements of clotting times (PT, aPTT), activities of antithrombin, protein C and clotting factors II-XII, and concentrations of fibrinogen and D-dimer were carried out before and 1 h, 24 h, 3 days and 7 days after scaffold implantation. The introduction of scaffold initially resulted in a slowdown of the clotting processes (most evident 24 h after surgery); PT and aPTT increased to 14.8 s and 33.9 s, respectively. From the third day onwards, most of these alterations began to return to normal values. The concentration of fibrinogen rose throughout the observation period (up to 8.4 g/L), mirroring the ongoing inflammatory reaction. However, no signals of significant disturbances in hemostatic processes were detected in the sheep tested. MDPI 2021-11-16 /pmc/articles/PMC8622787/ /pubmed/34832335 http://dx.doi.org/10.3390/ma14226934 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pliszczak-Król, Aleksandra
Kiełbowicz, Zdzisław
Król, Jarosław
Antończyk, Agnieszka
Gemra, Marianna
Skrzypczak, Piotr
Prządka, Przemysław
Zalewski, Dariusz
Bieżyński, Janusz
Nicpoń, Jakub
Parameters of Hemostasis in Sheep Implanted with Composite Scaffold Settled by Stimulated Mesenchymal Stem Cells—Evaluation of the Animal Model
title Parameters of Hemostasis in Sheep Implanted with Composite Scaffold Settled by Stimulated Mesenchymal Stem Cells—Evaluation of the Animal Model
title_full Parameters of Hemostasis in Sheep Implanted with Composite Scaffold Settled by Stimulated Mesenchymal Stem Cells—Evaluation of the Animal Model
title_fullStr Parameters of Hemostasis in Sheep Implanted with Composite Scaffold Settled by Stimulated Mesenchymal Stem Cells—Evaluation of the Animal Model
title_full_unstemmed Parameters of Hemostasis in Sheep Implanted with Composite Scaffold Settled by Stimulated Mesenchymal Stem Cells—Evaluation of the Animal Model
title_short Parameters of Hemostasis in Sheep Implanted with Composite Scaffold Settled by Stimulated Mesenchymal Stem Cells—Evaluation of the Animal Model
title_sort parameters of hemostasis in sheep implanted with composite scaffold settled by stimulated mesenchymal stem cells—evaluation of the animal model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8622787/
https://www.ncbi.nlm.nih.gov/pubmed/34832335
http://dx.doi.org/10.3390/ma14226934
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