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Tolerance and Persistence of Ebola Virus in Primary Cells from Mops condylurus, a Potential Ebola Virus Reservoir

Although there have been documented Ebola virus disease outbreaks for more than 40 years, the natural reservoir host has not been identified. Recent studies provide evidence that the Angolan free-tailed bat (Mops condylurus), an insectivorous microbat, is a possible ebolavirus reservoir. To investig...

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Autores principales: Bokelmann, Marcel, Vogel, Uwe, Debeljak, Franka, Düx, Ariane, Riesle-Sbarbaro, Silke, Lander, Angelika, Wahlbrink, Annette, Kromarek, Nicole, Neil, Stuart, Couacy-Hymann, Emmanuel, Prescott, Joseph, Kurth, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8622823/
https://www.ncbi.nlm.nih.gov/pubmed/34834992
http://dx.doi.org/10.3390/v13112186
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author Bokelmann, Marcel
Vogel, Uwe
Debeljak, Franka
Düx, Ariane
Riesle-Sbarbaro, Silke
Lander, Angelika
Wahlbrink, Annette
Kromarek, Nicole
Neil, Stuart
Couacy-Hymann, Emmanuel
Prescott, Joseph
Kurth, Andreas
author_facet Bokelmann, Marcel
Vogel, Uwe
Debeljak, Franka
Düx, Ariane
Riesle-Sbarbaro, Silke
Lander, Angelika
Wahlbrink, Annette
Kromarek, Nicole
Neil, Stuart
Couacy-Hymann, Emmanuel
Prescott, Joseph
Kurth, Andreas
author_sort Bokelmann, Marcel
collection PubMed
description Although there have been documented Ebola virus disease outbreaks for more than 40 years, the natural reservoir host has not been identified. Recent studies provide evidence that the Angolan free-tailed bat (Mops condylurus), an insectivorous microbat, is a possible ebolavirus reservoir. To investigate the potential role of this bat species in the ecology of ebolaviruses, replication, tolerance, and persistence of Ebola virus (EBOV) were investigated in 10 different primary bat cell isolates from M. condylurus. Varying EBOV replication kinetics corresponded to the expression levels of the integral membrane protein NPC1. All primary cells were highly tolerant to EBOV infection without cytopathic effects. The observed persistent EBOV infection for 150 days in lung primary cells, without resultant selective pressure leading to virus mutation, indicate the intrinsic ability of EBOV to persist in this bat species. These results provide further evidence for this bat species to be a likely reservoir of ebolaviruses.
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spelling pubmed-86228232021-11-27 Tolerance and Persistence of Ebola Virus in Primary Cells from Mops condylurus, a Potential Ebola Virus Reservoir Bokelmann, Marcel Vogel, Uwe Debeljak, Franka Düx, Ariane Riesle-Sbarbaro, Silke Lander, Angelika Wahlbrink, Annette Kromarek, Nicole Neil, Stuart Couacy-Hymann, Emmanuel Prescott, Joseph Kurth, Andreas Viruses Article Although there have been documented Ebola virus disease outbreaks for more than 40 years, the natural reservoir host has not been identified. Recent studies provide evidence that the Angolan free-tailed bat (Mops condylurus), an insectivorous microbat, is a possible ebolavirus reservoir. To investigate the potential role of this bat species in the ecology of ebolaviruses, replication, tolerance, and persistence of Ebola virus (EBOV) were investigated in 10 different primary bat cell isolates from M. condylurus. Varying EBOV replication kinetics corresponded to the expression levels of the integral membrane protein NPC1. All primary cells were highly tolerant to EBOV infection without cytopathic effects. The observed persistent EBOV infection for 150 days in lung primary cells, without resultant selective pressure leading to virus mutation, indicate the intrinsic ability of EBOV to persist in this bat species. These results provide further evidence for this bat species to be a likely reservoir of ebolaviruses. MDPI 2021-10-29 /pmc/articles/PMC8622823/ /pubmed/34834992 http://dx.doi.org/10.3390/v13112186 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bokelmann, Marcel
Vogel, Uwe
Debeljak, Franka
Düx, Ariane
Riesle-Sbarbaro, Silke
Lander, Angelika
Wahlbrink, Annette
Kromarek, Nicole
Neil, Stuart
Couacy-Hymann, Emmanuel
Prescott, Joseph
Kurth, Andreas
Tolerance and Persistence of Ebola Virus in Primary Cells from Mops condylurus, a Potential Ebola Virus Reservoir
title Tolerance and Persistence of Ebola Virus in Primary Cells from Mops condylurus, a Potential Ebola Virus Reservoir
title_full Tolerance and Persistence of Ebola Virus in Primary Cells from Mops condylurus, a Potential Ebola Virus Reservoir
title_fullStr Tolerance and Persistence of Ebola Virus in Primary Cells from Mops condylurus, a Potential Ebola Virus Reservoir
title_full_unstemmed Tolerance and Persistence of Ebola Virus in Primary Cells from Mops condylurus, a Potential Ebola Virus Reservoir
title_short Tolerance and Persistence of Ebola Virus in Primary Cells from Mops condylurus, a Potential Ebola Virus Reservoir
title_sort tolerance and persistence of ebola virus in primary cells from mops condylurus, a potential ebola virus reservoir
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8622823/
https://www.ncbi.nlm.nih.gov/pubmed/34834992
http://dx.doi.org/10.3390/v13112186
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