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Measurement of Lateral Transmission of Force in the Extensor Digitorum Longus Muscle of Young and Old Mice

The main function of skeletal muscles is to generate force. The force developed by myofiber contraction is transmitted to the tendon. There are two pathways of force transmission from myofibers to tendons: longitudinal transmission that depends on tension elicited via the myotendinous junction and l...

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Autores principales: Minato, Keitaro, Yoshimoto, Yuki, Kurosawa, Tamaki, Watanabe, Kei, Kawashima, Hiroyuki, Ikemoto-Uezumi, Madoka, Uezumi, Akiyoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8623005/
https://www.ncbi.nlm.nih.gov/pubmed/34830237
http://dx.doi.org/10.3390/ijms222212356
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author Minato, Keitaro
Yoshimoto, Yuki
Kurosawa, Tamaki
Watanabe, Kei
Kawashima, Hiroyuki
Ikemoto-Uezumi, Madoka
Uezumi, Akiyoshi
author_facet Minato, Keitaro
Yoshimoto, Yuki
Kurosawa, Tamaki
Watanabe, Kei
Kawashima, Hiroyuki
Ikemoto-Uezumi, Madoka
Uezumi, Akiyoshi
author_sort Minato, Keitaro
collection PubMed
description The main function of skeletal muscles is to generate force. The force developed by myofiber contraction is transmitted to the tendon. There are two pathways of force transmission from myofibers to tendons: longitudinal transmission that depends on tension elicited via the myotendinous junction and lateral transmission that depends on shear elicited via the interface between the myofiber surface and surrounding connective tissue. Experiments using animal muscle and mathematical models indicated that lateral transmission is the dominant pathway in muscle force transmission. Studies using rat muscle showed that the efficiency of lateral force transmission declines with age. Here, the lateral transmission of force was measured using the extensor digitorum longus muscle from young and old mice. Dependence on longitudinal transmission increased in the old muscle, and there was a trend for lower efficiency of lateral force transmission in the old muscle compared to the young muscle. There was a noticeable increase in the connective tissue volume in the old muscle; however, there was no significant change in the expression of dystrophin, a critical molecule for the link between the myofiber cytoskeleton and extracellular matrix. This study demonstrates the measurement of lateral force transmission in mouse muscles and that alteration in force transmission property may underlie age-related muscle weakness.
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spelling pubmed-86230052021-11-27 Measurement of Lateral Transmission of Force in the Extensor Digitorum Longus Muscle of Young and Old Mice Minato, Keitaro Yoshimoto, Yuki Kurosawa, Tamaki Watanabe, Kei Kawashima, Hiroyuki Ikemoto-Uezumi, Madoka Uezumi, Akiyoshi Int J Mol Sci Communication The main function of skeletal muscles is to generate force. The force developed by myofiber contraction is transmitted to the tendon. There are two pathways of force transmission from myofibers to tendons: longitudinal transmission that depends on tension elicited via the myotendinous junction and lateral transmission that depends on shear elicited via the interface between the myofiber surface and surrounding connective tissue. Experiments using animal muscle and mathematical models indicated that lateral transmission is the dominant pathway in muscle force transmission. Studies using rat muscle showed that the efficiency of lateral force transmission declines with age. Here, the lateral transmission of force was measured using the extensor digitorum longus muscle from young and old mice. Dependence on longitudinal transmission increased in the old muscle, and there was a trend for lower efficiency of lateral force transmission in the old muscle compared to the young muscle. There was a noticeable increase in the connective tissue volume in the old muscle; however, there was no significant change in the expression of dystrophin, a critical molecule for the link between the myofiber cytoskeleton and extracellular matrix. This study demonstrates the measurement of lateral force transmission in mouse muscles and that alteration in force transmission property may underlie age-related muscle weakness. MDPI 2021-11-16 /pmc/articles/PMC8623005/ /pubmed/34830237 http://dx.doi.org/10.3390/ijms222212356 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Minato, Keitaro
Yoshimoto, Yuki
Kurosawa, Tamaki
Watanabe, Kei
Kawashima, Hiroyuki
Ikemoto-Uezumi, Madoka
Uezumi, Akiyoshi
Measurement of Lateral Transmission of Force in the Extensor Digitorum Longus Muscle of Young and Old Mice
title Measurement of Lateral Transmission of Force in the Extensor Digitorum Longus Muscle of Young and Old Mice
title_full Measurement of Lateral Transmission of Force in the Extensor Digitorum Longus Muscle of Young and Old Mice
title_fullStr Measurement of Lateral Transmission of Force in the Extensor Digitorum Longus Muscle of Young and Old Mice
title_full_unstemmed Measurement of Lateral Transmission of Force in the Extensor Digitorum Longus Muscle of Young and Old Mice
title_short Measurement of Lateral Transmission of Force in the Extensor Digitorum Longus Muscle of Young and Old Mice
title_sort measurement of lateral transmission of force in the extensor digitorum longus muscle of young and old mice
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8623005/
https://www.ncbi.nlm.nih.gov/pubmed/34830237
http://dx.doi.org/10.3390/ijms222212356
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