Cargando…

Susceptibility of Field-Collected Nyssorhynchus darlingi to Plasmodium spp. in Western Amazonian Brazil

Mosquito susceptibility to Plasmodium spp. infection is of paramount importance for malaria occurrence and sustainable transmission. Therefore, understanding the genetic features underlying the mechanisms of susceptibility traits is pivotal to assessing malaria transmission dynamics in endemic areas...

Descripción completa

Detalles Bibliográficos
Autores principales: Alonso, Diego Peres, Alvarez, Marcus Vinicius Niz, Ribolla, Paulo Eduardo Martins, Conn, Jan E., de Oliveira, Tatiane Marques Porangaba, Sallum, Maria Anice Mureb
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8623036/
https://www.ncbi.nlm.nih.gov/pubmed/34828299
http://dx.doi.org/10.3390/genes12111693
_version_ 1784605835403460608
author Alonso, Diego Peres
Alvarez, Marcus Vinicius Niz
Ribolla, Paulo Eduardo Martins
Conn, Jan E.
de Oliveira, Tatiane Marques Porangaba
Sallum, Maria Anice Mureb
author_facet Alonso, Diego Peres
Alvarez, Marcus Vinicius Niz
Ribolla, Paulo Eduardo Martins
Conn, Jan E.
de Oliveira, Tatiane Marques Porangaba
Sallum, Maria Anice Mureb
author_sort Alonso, Diego Peres
collection PubMed
description Mosquito susceptibility to Plasmodium spp. infection is of paramount importance for malaria occurrence and sustainable transmission. Therefore, understanding the genetic features underlying the mechanisms of susceptibility traits is pivotal to assessing malaria transmission dynamics in endemic areas. The aim of this study was to investigate the susceptibility of Nyssorhynchus darlingi—the dominant malaria vector in Brazil—to Plasmodium spp. using a reduced representation genome-sequencing protocol. The investigation was performed using a genome-wide association study (GWAS) to identify mosquito genes that are predicted to modulate the susceptibility of natural populations of the mosquito to Plasmodium infection. After applying the sequence alignment protocol, we generated the variant panel and filtered variants; leading to the detection of 202,837 SNPs in all specimens analyzed. The resulting panel was used to perform GWAS by comparing the pool of SNP variants present in Ny. darlingi infected with Plasmodium spp. with the pool obtained in field-collected mosquitoes with no evidence of infection by the parasite (all mosquitoes were tested separately using RT-PCR). The GWAS results for infection status showed two statistically significant variants adjacent to important genes that can be associated with susceptibility to Plasmodium infection: Cytochrome P450 (cyp450) and chitinase. This study provides relevant knowledge on malaria transmission dynamics by using a genomic approach to identify mosquito genes associated with susceptibility to Plasmodium infection in Ny. darlingi in western Amazonian Brazil.
format Online
Article
Text
id pubmed-8623036
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-86230362021-11-27 Susceptibility of Field-Collected Nyssorhynchus darlingi to Plasmodium spp. in Western Amazonian Brazil Alonso, Diego Peres Alvarez, Marcus Vinicius Niz Ribolla, Paulo Eduardo Martins Conn, Jan E. de Oliveira, Tatiane Marques Porangaba Sallum, Maria Anice Mureb Genes (Basel) Article Mosquito susceptibility to Plasmodium spp. infection is of paramount importance for malaria occurrence and sustainable transmission. Therefore, understanding the genetic features underlying the mechanisms of susceptibility traits is pivotal to assessing malaria transmission dynamics in endemic areas. The aim of this study was to investigate the susceptibility of Nyssorhynchus darlingi—the dominant malaria vector in Brazil—to Plasmodium spp. using a reduced representation genome-sequencing protocol. The investigation was performed using a genome-wide association study (GWAS) to identify mosquito genes that are predicted to modulate the susceptibility of natural populations of the mosquito to Plasmodium infection. After applying the sequence alignment protocol, we generated the variant panel and filtered variants; leading to the detection of 202,837 SNPs in all specimens analyzed. The resulting panel was used to perform GWAS by comparing the pool of SNP variants present in Ny. darlingi infected with Plasmodium spp. with the pool obtained in field-collected mosquitoes with no evidence of infection by the parasite (all mosquitoes were tested separately using RT-PCR). The GWAS results for infection status showed two statistically significant variants adjacent to important genes that can be associated with susceptibility to Plasmodium infection: Cytochrome P450 (cyp450) and chitinase. This study provides relevant knowledge on malaria transmission dynamics by using a genomic approach to identify mosquito genes associated with susceptibility to Plasmodium infection in Ny. darlingi in western Amazonian Brazil. MDPI 2021-10-25 /pmc/articles/PMC8623036/ /pubmed/34828299 http://dx.doi.org/10.3390/genes12111693 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Alonso, Diego Peres
Alvarez, Marcus Vinicius Niz
Ribolla, Paulo Eduardo Martins
Conn, Jan E.
de Oliveira, Tatiane Marques Porangaba
Sallum, Maria Anice Mureb
Susceptibility of Field-Collected Nyssorhynchus darlingi to Plasmodium spp. in Western Amazonian Brazil
title Susceptibility of Field-Collected Nyssorhynchus darlingi to Plasmodium spp. in Western Amazonian Brazil
title_full Susceptibility of Field-Collected Nyssorhynchus darlingi to Plasmodium spp. in Western Amazonian Brazil
title_fullStr Susceptibility of Field-Collected Nyssorhynchus darlingi to Plasmodium spp. in Western Amazonian Brazil
title_full_unstemmed Susceptibility of Field-Collected Nyssorhynchus darlingi to Plasmodium spp. in Western Amazonian Brazil
title_short Susceptibility of Field-Collected Nyssorhynchus darlingi to Plasmodium spp. in Western Amazonian Brazil
title_sort susceptibility of field-collected nyssorhynchus darlingi to plasmodium spp. in western amazonian brazil
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8623036/
https://www.ncbi.nlm.nih.gov/pubmed/34828299
http://dx.doi.org/10.3390/genes12111693
work_keys_str_mv AT alonsodiegoperes susceptibilityoffieldcollectednyssorhynchusdarlingitoplasmodiumsppinwesternamazonianbrazil
AT alvarezmarcusviniciusniz susceptibilityoffieldcollectednyssorhynchusdarlingitoplasmodiumsppinwesternamazonianbrazil
AT ribollapauloeduardomartins susceptibilityoffieldcollectednyssorhynchusdarlingitoplasmodiumsppinwesternamazonianbrazil
AT connjane susceptibilityoffieldcollectednyssorhynchusdarlingitoplasmodiumsppinwesternamazonianbrazil
AT deoliveiratatianemarquesporangaba susceptibilityoffieldcollectednyssorhynchusdarlingitoplasmodiumsppinwesternamazonianbrazil
AT sallummariaanicemureb susceptibilityoffieldcollectednyssorhynchusdarlingitoplasmodiumsppinwesternamazonianbrazil