Cargando…

Bluetongue Virus Infections in Cattle Herds of Manabí Province of Ecuador

Bluetongue (BT) is a viral disease transmitted by Culicoides (Diptera: Ceratopogonidae) to domestic and wild ruminants. Infections in cattle are mainly subclinical, but severe necrotic and hemorrhagic illness and death may occur depending on the strain of the virus and other factors; cattle act as a...

Descripción completa

Detalles Bibliográficos
Autores principales: De la Torre, Euclides, Moreira, Nixon, Saegerman, Claude, De Clercq, Kris, Salinas, María, Maldonado, Alex, Jarrín, David, Sol Vaca, María, Pachacama, Silvia, Espinoza, Jorge, Delgado, Hipatia, Barrera, Maritza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8623054/
https://www.ncbi.nlm.nih.gov/pubmed/34832601
http://dx.doi.org/10.3390/pathogens10111445
_version_ 1784605839772876800
author De la Torre, Euclides
Moreira, Nixon
Saegerman, Claude
De Clercq, Kris
Salinas, María
Maldonado, Alex
Jarrín, David
Sol Vaca, María
Pachacama, Silvia
Espinoza, Jorge
Delgado, Hipatia
Barrera, Maritza
author_facet De la Torre, Euclides
Moreira, Nixon
Saegerman, Claude
De Clercq, Kris
Salinas, María
Maldonado, Alex
Jarrín, David
Sol Vaca, María
Pachacama, Silvia
Espinoza, Jorge
Delgado, Hipatia
Barrera, Maritza
author_sort De la Torre, Euclides
collection PubMed
description Bluetongue (BT) is a viral disease transmitted by Culicoides (Diptera: Ceratopogonidae) to domestic and wild ruminants. Infections in cattle are mainly subclinical, but severe necrotic and hemorrhagic illness and death may occur depending on the strain of the virus and other factors; cattle act as a reservoir for the virus. Although the Ecuadorian coast has climatic conditions that favor the presence of the vector, there are few serologic or virologic BTV studies available. Manabí is a coastal province in which livestock farming is mostly implemented in the northern part. We conducted two studies to assess, for the first time, the presence of active BTV infections in Manabí province. We collected 430 serum samples from 38 randomly selected farms between March and July 2019 to perform BTV competitive ELISA. In addition, six seropositive farms were selected to place eight sentinel BTV-naive calves. All these calves were blood sampled and the presence of BTV RNA and antibodies was tested for by RT-PCR and competitive ELISA, respectively, once a week for 6–8 weeks until seroconversion was evidenced. A high individual seroprevalence (99%) was obtained, and all investigated farms had BTV seropositive animals. All sentinel calves became BTV viremic and seroconverted. The first viremia appeared after 2–5 weeks from arrival at the farm; they seroconverted 1–3 weeks later. We demonstrate for the first time that there is a high level of BTV circulation north of Manabí, with active infections on these farms. Integrated control strategies such as hygienic measures on farms to reduce midge populations would be advisable for the owners as mitigation measures.
format Online
Article
Text
id pubmed-8623054
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-86230542021-11-27 Bluetongue Virus Infections in Cattle Herds of Manabí Province of Ecuador De la Torre, Euclides Moreira, Nixon Saegerman, Claude De Clercq, Kris Salinas, María Maldonado, Alex Jarrín, David Sol Vaca, María Pachacama, Silvia Espinoza, Jorge Delgado, Hipatia Barrera, Maritza Pathogens Article Bluetongue (BT) is a viral disease transmitted by Culicoides (Diptera: Ceratopogonidae) to domestic and wild ruminants. Infections in cattle are mainly subclinical, but severe necrotic and hemorrhagic illness and death may occur depending on the strain of the virus and other factors; cattle act as a reservoir for the virus. Although the Ecuadorian coast has climatic conditions that favor the presence of the vector, there are few serologic or virologic BTV studies available. Manabí is a coastal province in which livestock farming is mostly implemented in the northern part. We conducted two studies to assess, for the first time, the presence of active BTV infections in Manabí province. We collected 430 serum samples from 38 randomly selected farms between March and July 2019 to perform BTV competitive ELISA. In addition, six seropositive farms were selected to place eight sentinel BTV-naive calves. All these calves were blood sampled and the presence of BTV RNA and antibodies was tested for by RT-PCR and competitive ELISA, respectively, once a week for 6–8 weeks until seroconversion was evidenced. A high individual seroprevalence (99%) was obtained, and all investigated farms had BTV seropositive animals. All sentinel calves became BTV viremic and seroconverted. The first viremia appeared after 2–5 weeks from arrival at the farm; they seroconverted 1–3 weeks later. We demonstrate for the first time that there is a high level of BTV circulation north of Manabí, with active infections on these farms. Integrated control strategies such as hygienic measures on farms to reduce midge populations would be advisable for the owners as mitigation measures. MDPI 2021-11-06 /pmc/articles/PMC8623054/ /pubmed/34832601 http://dx.doi.org/10.3390/pathogens10111445 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
De la Torre, Euclides
Moreira, Nixon
Saegerman, Claude
De Clercq, Kris
Salinas, María
Maldonado, Alex
Jarrín, David
Sol Vaca, María
Pachacama, Silvia
Espinoza, Jorge
Delgado, Hipatia
Barrera, Maritza
Bluetongue Virus Infections in Cattle Herds of Manabí Province of Ecuador
title Bluetongue Virus Infections in Cattle Herds of Manabí Province of Ecuador
title_full Bluetongue Virus Infections in Cattle Herds of Manabí Province of Ecuador
title_fullStr Bluetongue Virus Infections in Cattle Herds of Manabí Province of Ecuador
title_full_unstemmed Bluetongue Virus Infections in Cattle Herds of Manabí Province of Ecuador
title_short Bluetongue Virus Infections in Cattle Herds of Manabí Province of Ecuador
title_sort bluetongue virus infections in cattle herds of manabí province of ecuador
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8623054/
https://www.ncbi.nlm.nih.gov/pubmed/34832601
http://dx.doi.org/10.3390/pathogens10111445
work_keys_str_mv AT delatorreeuclides bluetonguevirusinfectionsincattleherdsofmanabiprovinceofecuador
AT moreiranixon bluetonguevirusinfectionsincattleherdsofmanabiprovinceofecuador
AT saegermanclaude bluetonguevirusinfectionsincattleherdsofmanabiprovinceofecuador
AT declercqkris bluetonguevirusinfectionsincattleherdsofmanabiprovinceofecuador
AT salinasmaria bluetonguevirusinfectionsincattleherdsofmanabiprovinceofecuador
AT maldonadoalex bluetonguevirusinfectionsincattleherdsofmanabiprovinceofecuador
AT jarrindavid bluetonguevirusinfectionsincattleherdsofmanabiprovinceofecuador
AT solvacamaria bluetonguevirusinfectionsincattleherdsofmanabiprovinceofecuador
AT pachacamasilvia bluetonguevirusinfectionsincattleherdsofmanabiprovinceofecuador
AT espinozajorge bluetonguevirusinfectionsincattleherdsofmanabiprovinceofecuador
AT delgadohipatia bluetonguevirusinfectionsincattleherdsofmanabiprovinceofecuador
AT barreramaritza bluetonguevirusinfectionsincattleherdsofmanabiprovinceofecuador