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Assessing Asymptomatic Malaria Carriage of Plasmodium falciparum and Non-falciparum Species in Children Resident in Nkolbisson, Yaoundé, Cameroon
Malaria is still a threat to public health as it remains the first endemic disease in the world. It is a pervasive parasitic disease in tropical and subtropical regions where asymptomatic malaria infection among humans serves as a significant reservoir for transmission. A rapid and correct diagnosis...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8623063/ https://www.ncbi.nlm.nih.gov/pubmed/34828673 http://dx.doi.org/10.3390/children8110960 |
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author | Akindeh, Nji Mbuh Ngum, Lesley Ngum Niba, Peter Thelma Ngwa Ali, Innocent Mbulli Ayem, Ornella Laetitia Oben Chedjou, Jean Paul Kengne Fomboh, Calvino Tah Ekollo, Aristid Herve Mbange Mbu’u, Cyrille Mbanwi Mbacham, Wilfred Fon |
author_facet | Akindeh, Nji Mbuh Ngum, Lesley Ngum Niba, Peter Thelma Ngwa Ali, Innocent Mbulli Ayem, Ornella Laetitia Oben Chedjou, Jean Paul Kengne Fomboh, Calvino Tah Ekollo, Aristid Herve Mbange Mbu’u, Cyrille Mbanwi Mbacham, Wilfred Fon |
author_sort | Akindeh, Nji Mbuh |
collection | PubMed |
description | Malaria is still a threat to public health as it remains the first endemic disease in the world. It is a pervasive parasitic disease in tropical and subtropical regions where asymptomatic malaria infection among humans serves as a significant reservoir for transmission. A rapid and correct diagnosis is considered to be an important strategy in the control of the disease especially in children, who are the most vulnerable group. This study assessed the prevalence of asymptomatic malaria in children at the Nkolbisson health area in Yaoundé, Cameroon. A cross-sectional study design and a convenience sampling plan were used. A total of 127 participants were recruited after informed and signed consent from parents and/or guardians. Blood samples were collected by finger-pricking and venipuncture from children aged 6 months to 10 years and then screened for asymptomatic parasitemia by a rapid diagnostic test (RDT), light microscopy (LM) staining with Giemsa and 18S rRNA polymerase chain reaction (PCR) for speciation. The data were analyzed using SPSS version 20 software. The study identified 85 children who were positive from the PCR, 95 positive from the RDT and 71 from the LM, revealing a malaria prevalence of 66.9%, 74.8% and 55.9%, respectively. The prevalence was not observed to be dependent on the sex and age group of the participants. Plasmodium falciparum was the predominant species followed by Plasmodium malariae and then Plasmodium ovale. The RDT and LM had the same sensitivity (90.6%) with a slight difference in their specificity (RDT: 57.1%; LM: 54.8%). The RDT also demonstrated higher positive and negative predictive values compared with those of the LM. |
format | Online Article Text |
id | pubmed-8623063 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-86230632021-11-27 Assessing Asymptomatic Malaria Carriage of Plasmodium falciparum and Non-falciparum Species in Children Resident in Nkolbisson, Yaoundé, Cameroon Akindeh, Nji Mbuh Ngum, Lesley Ngum Niba, Peter Thelma Ngwa Ali, Innocent Mbulli Ayem, Ornella Laetitia Oben Chedjou, Jean Paul Kengne Fomboh, Calvino Tah Ekollo, Aristid Herve Mbange Mbu’u, Cyrille Mbanwi Mbacham, Wilfred Fon Children (Basel) Article Malaria is still a threat to public health as it remains the first endemic disease in the world. It is a pervasive parasitic disease in tropical and subtropical regions where asymptomatic malaria infection among humans serves as a significant reservoir for transmission. A rapid and correct diagnosis is considered to be an important strategy in the control of the disease especially in children, who are the most vulnerable group. This study assessed the prevalence of asymptomatic malaria in children at the Nkolbisson health area in Yaoundé, Cameroon. A cross-sectional study design and a convenience sampling plan were used. A total of 127 participants were recruited after informed and signed consent from parents and/or guardians. Blood samples were collected by finger-pricking and venipuncture from children aged 6 months to 10 years and then screened for asymptomatic parasitemia by a rapid diagnostic test (RDT), light microscopy (LM) staining with Giemsa and 18S rRNA polymerase chain reaction (PCR) for speciation. The data were analyzed using SPSS version 20 software. The study identified 85 children who were positive from the PCR, 95 positive from the RDT and 71 from the LM, revealing a malaria prevalence of 66.9%, 74.8% and 55.9%, respectively. The prevalence was not observed to be dependent on the sex and age group of the participants. Plasmodium falciparum was the predominant species followed by Plasmodium malariae and then Plasmodium ovale. The RDT and LM had the same sensitivity (90.6%) with a slight difference in their specificity (RDT: 57.1%; LM: 54.8%). The RDT also demonstrated higher positive and negative predictive values compared with those of the LM. MDPI 2021-10-24 /pmc/articles/PMC8623063/ /pubmed/34828673 http://dx.doi.org/10.3390/children8110960 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Akindeh, Nji Mbuh Ngum, Lesley Ngum Niba, Peter Thelma Ngwa Ali, Innocent Mbulli Ayem, Ornella Laetitia Oben Chedjou, Jean Paul Kengne Fomboh, Calvino Tah Ekollo, Aristid Herve Mbange Mbu’u, Cyrille Mbanwi Mbacham, Wilfred Fon Assessing Asymptomatic Malaria Carriage of Plasmodium falciparum and Non-falciparum Species in Children Resident in Nkolbisson, Yaoundé, Cameroon |
title | Assessing Asymptomatic Malaria Carriage of Plasmodium falciparum and Non-falciparum Species in Children Resident in Nkolbisson, Yaoundé, Cameroon |
title_full | Assessing Asymptomatic Malaria Carriage of Plasmodium falciparum and Non-falciparum Species in Children Resident in Nkolbisson, Yaoundé, Cameroon |
title_fullStr | Assessing Asymptomatic Malaria Carriage of Plasmodium falciparum and Non-falciparum Species in Children Resident in Nkolbisson, Yaoundé, Cameroon |
title_full_unstemmed | Assessing Asymptomatic Malaria Carriage of Plasmodium falciparum and Non-falciparum Species in Children Resident in Nkolbisson, Yaoundé, Cameroon |
title_short | Assessing Asymptomatic Malaria Carriage of Plasmodium falciparum and Non-falciparum Species in Children Resident in Nkolbisson, Yaoundé, Cameroon |
title_sort | assessing asymptomatic malaria carriage of plasmodium falciparum and non-falciparum species in children resident in nkolbisson, yaoundé, cameroon |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8623063/ https://www.ncbi.nlm.nih.gov/pubmed/34828673 http://dx.doi.org/10.3390/children8110960 |
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