Cargando…

High Individual Heterogeneity of Neutralizing Activities against the Original Strain and Nine Different Variants of SARS-CoV-2

Background: Since the beginning of the COVID-19 pandemic, several SARS-CoV-2 variants have sequentially emerged. In France, most cases were due to spike D641G-harbouring viruses that descended initially from the Wuhan strain, then by the variant of B.1.160 lineage we called Marseille-4 since the sum...

Descripción completa

Detalles Bibliográficos
Autores principales: Jaafar, Rita, Boschi, Celine, Aherfi, Sarah, Bancod, Audrey, Le Bideau, Marion, Edouard, Sophie, Colson, Philippe, Chahinian, Henri, Raoult, Didier, Yahi, Nouara, Fantini, Jacques, La Scola, Bernard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8623169/
https://www.ncbi.nlm.nih.gov/pubmed/34834983
http://dx.doi.org/10.3390/v13112177
_version_ 1784605867346231296
author Jaafar, Rita
Boschi, Celine
Aherfi, Sarah
Bancod, Audrey
Le Bideau, Marion
Edouard, Sophie
Colson, Philippe
Chahinian, Henri
Raoult, Didier
Yahi, Nouara
Fantini, Jacques
La Scola, Bernard
author_facet Jaafar, Rita
Boschi, Celine
Aherfi, Sarah
Bancod, Audrey
Le Bideau, Marion
Edouard, Sophie
Colson, Philippe
Chahinian, Henri
Raoult, Didier
Yahi, Nouara
Fantini, Jacques
La Scola, Bernard
author_sort Jaafar, Rita
collection PubMed
description Background: Since the beginning of the COVID-19 pandemic, several SARS-CoV-2 variants have sequentially emerged. In France, most cases were due to spike D641G-harbouring viruses that descended initially from the Wuhan strain, then by the variant of B.1.160 lineage we called Marseille-4 since the summer of 2020, which was followed by the Alpha and Beta variants in early 2021, then the Delta variant currently. Methods: We determined the neutralising antibody (nAb) titres in sera from convalescent individuals previously infected by these four major local variants and from vaccine recipients to the original Wuhan strain and nine variants, including two recent circulating Delta isolates. Results: The results show high inter-individual heterogeneity in nAbs, especially according to the variant tested. The major variations among nAbs are based on the genotype responsible for the infection. Patients previously infected with the beta and B.1.160 variants had the lowest nAb titres. We show that this heterogeneity is well explained by spike protein mutants modelling using in silico approaches. The highest titres were observed in individuals vaccinated with the Pfizer/BioNTech COVID-19 vaccine, even against the delta variant. Conclusions: Immunity acquired naturally after infection is highly dependent on the infecting variant, and, unexpectedly, mRNA-based vaccine efficacy was shown to be often better than natural immunity in eliciting neutralising antibodies.
format Online
Article
Text
id pubmed-8623169
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-86231692021-11-27 High Individual Heterogeneity of Neutralizing Activities against the Original Strain and Nine Different Variants of SARS-CoV-2 Jaafar, Rita Boschi, Celine Aherfi, Sarah Bancod, Audrey Le Bideau, Marion Edouard, Sophie Colson, Philippe Chahinian, Henri Raoult, Didier Yahi, Nouara Fantini, Jacques La Scola, Bernard Viruses Article Background: Since the beginning of the COVID-19 pandemic, several SARS-CoV-2 variants have sequentially emerged. In France, most cases were due to spike D641G-harbouring viruses that descended initially from the Wuhan strain, then by the variant of B.1.160 lineage we called Marseille-4 since the summer of 2020, which was followed by the Alpha and Beta variants in early 2021, then the Delta variant currently. Methods: We determined the neutralising antibody (nAb) titres in sera from convalescent individuals previously infected by these four major local variants and from vaccine recipients to the original Wuhan strain and nine variants, including two recent circulating Delta isolates. Results: The results show high inter-individual heterogeneity in nAbs, especially according to the variant tested. The major variations among nAbs are based on the genotype responsible for the infection. Patients previously infected with the beta and B.1.160 variants had the lowest nAb titres. We show that this heterogeneity is well explained by spike protein mutants modelling using in silico approaches. The highest titres were observed in individuals vaccinated with the Pfizer/BioNTech COVID-19 vaccine, even against the delta variant. Conclusions: Immunity acquired naturally after infection is highly dependent on the infecting variant, and, unexpectedly, mRNA-based vaccine efficacy was shown to be often better than natural immunity in eliciting neutralising antibodies. MDPI 2021-10-28 /pmc/articles/PMC8623169/ /pubmed/34834983 http://dx.doi.org/10.3390/v13112177 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jaafar, Rita
Boschi, Celine
Aherfi, Sarah
Bancod, Audrey
Le Bideau, Marion
Edouard, Sophie
Colson, Philippe
Chahinian, Henri
Raoult, Didier
Yahi, Nouara
Fantini, Jacques
La Scola, Bernard
High Individual Heterogeneity of Neutralizing Activities against the Original Strain and Nine Different Variants of SARS-CoV-2
title High Individual Heterogeneity of Neutralizing Activities against the Original Strain and Nine Different Variants of SARS-CoV-2
title_full High Individual Heterogeneity of Neutralizing Activities against the Original Strain and Nine Different Variants of SARS-CoV-2
title_fullStr High Individual Heterogeneity of Neutralizing Activities against the Original Strain and Nine Different Variants of SARS-CoV-2
title_full_unstemmed High Individual Heterogeneity of Neutralizing Activities against the Original Strain and Nine Different Variants of SARS-CoV-2
title_short High Individual Heterogeneity of Neutralizing Activities against the Original Strain and Nine Different Variants of SARS-CoV-2
title_sort high individual heterogeneity of neutralizing activities against the original strain and nine different variants of sars-cov-2
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8623169/
https://www.ncbi.nlm.nih.gov/pubmed/34834983
http://dx.doi.org/10.3390/v13112177
work_keys_str_mv AT jaafarrita highindividualheterogeneityofneutralizingactivitiesagainsttheoriginalstrainandninedifferentvariantsofsarscov2
AT boschiceline highindividualheterogeneityofneutralizingactivitiesagainsttheoriginalstrainandninedifferentvariantsofsarscov2
AT aherfisarah highindividualheterogeneityofneutralizingactivitiesagainsttheoriginalstrainandninedifferentvariantsofsarscov2
AT bancodaudrey highindividualheterogeneityofneutralizingactivitiesagainsttheoriginalstrainandninedifferentvariantsofsarscov2
AT lebideaumarion highindividualheterogeneityofneutralizingactivitiesagainsttheoriginalstrainandninedifferentvariantsofsarscov2
AT edouardsophie highindividualheterogeneityofneutralizingactivitiesagainsttheoriginalstrainandninedifferentvariantsofsarscov2
AT colsonphilippe highindividualheterogeneityofneutralizingactivitiesagainsttheoriginalstrainandninedifferentvariantsofsarscov2
AT chahinianhenri highindividualheterogeneityofneutralizingactivitiesagainsttheoriginalstrainandninedifferentvariantsofsarscov2
AT raoultdidier highindividualheterogeneityofneutralizingactivitiesagainsttheoriginalstrainandninedifferentvariantsofsarscov2
AT yahinouara highindividualheterogeneityofneutralizingactivitiesagainsttheoriginalstrainandninedifferentvariantsofsarscov2
AT fantinijacques highindividualheterogeneityofneutralizingactivitiesagainsttheoriginalstrainandninedifferentvariantsofsarscov2
AT lascolabernard highindividualheterogeneityofneutralizingactivitiesagainsttheoriginalstrainandninedifferentvariantsofsarscov2