The Impact of the Entomopathogenic Fungus Conidiobolus coronatus on the Free Fatty Acid Profile of the Flesh Fly Sarcophaga argyrostoma

SIMPLE SUMMARY: The interaction between insect and fungus is characterised on the one hand by the parasite developing more effective strategies of host exploitation, and on the other, by the host mounting increasingly robust defences though Red Queen dynamics or coevolutionary arms races. Furthermore, depending on gene flow and differences in selection pressure between sites, both host and parasite may demonstrate local adaptation to their counterpart or develop more general resistance or offensive traits. As the cuticle is considered the first line of defence of the insect, changes in the FFA profile may well influence susceptibility or resistance to fungal invasion. Our findings indicate that Sarcophaga argyrostoma demonstrates stage-specific resistance to Conidiobolus coronatus infection and suggests that FFAs play a role in resistance to fungal infection in flesh flies. These findings not only increase our knowledge of the entomopatogenic potential of fungi, but also of the growing level of infection by C. coronatus in humans and other mammals. Also, the presented research suggests that FFAs demonstrate antifungal activity which may be helpful in designing new antifungal treatments. ABSTRACT: The chemical composition of the insect cuticle varies remarkably between species and their life stages. It can affect host resistance and substrate utilization by invading entomopathogen fungi, such as the soil fungus Conidiobolus coronatus. In this study, Sarcophaga argyrostoma flies were exposed to sporulating C. coronatus colonies for 24 h; the pupae were resistant, but the adults demonstrated 60% mortality. Although the pupae demonstrated no sign of infection nor any abnormal development, our findings indicate that after 24 h of contact with the fungus, the pupae demonstrated a 25.2-fold increase in total cuticular free fatty acids (FFAs) and a 1.9-fold decrease in total internal FFAs. Also, the cuticular FFA increased from 26 to 30, while the internal FFA class increased from 13 to 23. In exposed adults, the total mass of cuticular FFAs increased 1.7-fold, while the number of FFAs stayed the same (32 FFAs). Also, the internal FFA class increased from 26 to 35 and the total FFA mass increased 1.1-fold. These considerable differences between adults and pupae associated with C. coronatus exposure indicate developmental changes in the mechanisms governing lipid metabolism and spatial distribution in the organism, and suggest that cuticular lipids play a vital role in the defence against pathogenic fungi.