Cargando…

Astragalus Polysaccharides and Saponins Alleviate Liver Injury and Regulate Gut Microbiota in Alcohol Liver Disease Mice

Astragalus, a medical and edible plant in China, shows several bioactive properties. However, the role of astragalus in attenuating alcoholic liver disease (ALD) is less clear. The objective of this project is to investigate the improving effect of astragalus saponins (AS) and astragalus polysacchar...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhou, Jingxuan, Zhang, Nanhai, Zhao, Liang, Wu, Wei, Zhang, Liebing, Zhou, Feng, Li, Jingming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8623381/
https://www.ncbi.nlm.nih.gov/pubmed/34828972
http://dx.doi.org/10.3390/foods10112688
_version_ 1784605919406981120
author Zhou, Jingxuan
Zhang, Nanhai
Zhao, Liang
Wu, Wei
Zhang, Liebing
Zhou, Feng
Li, Jingming
author_facet Zhou, Jingxuan
Zhang, Nanhai
Zhao, Liang
Wu, Wei
Zhang, Liebing
Zhou, Feng
Li, Jingming
author_sort Zhou, Jingxuan
collection PubMed
description Astragalus, a medical and edible plant in China, shows several bioactive properties. However, the role of astragalus in attenuating alcoholic liver disease (ALD) is less clear. The objective of this project is to investigate the improving effect of astragalus saponins (AS) and astragalus polysaccharides (AP), which are the two primary constituents in astragalus on hepatic injury induced by alcohol, and the potential mechanisms of action. Different doses of AS (50 and 100 mg/kg bw) and AP (300 and 600 mg/kg bw) were orally given to alcohol-treated mice for four weeks. The results demonstrated that both AP and AS could reverse the increase of the levels of TC, TG, FFA, and LDL-C in serum, and the decrease of serum HDL-C content, as well as the elevation of hepatic TC and TG levels induced by alcohol. The activities of AST, ALT, ALP, and γ-GT in ALD mice were raised after AP and AS supplementation. The antioxidant markers (SOD, CAT, GSH, and GSH-Px) were obviously augmented and the pro-inflammatory cytokines (TNF-α, IL-6 and IL-1β) and hepatic histological variations were alleviated by AP and AS, which was in line with the levels of oxidative stress-associated genes (Keap1, Nfe2l2, Nqo1, and Hmox1) and inflammation-associated genes (Tlr4, Myd88 and Nfkb1). In addition, AS exerted a more efficient effect than AP and the results presented dose proportionality. Moreover, AS and AP could modulate the intestinal microbiota disturbance induced by alcohol. Overall, AS and AP administration could ameliorate lipid accumulation in the serum and liver, as well as hepatic function, oxidative stress, inflammatory response, and gut flora disorders in mice as a result of alcohol.
format Online
Article
Text
id pubmed-8623381
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-86233812021-11-27 Astragalus Polysaccharides and Saponins Alleviate Liver Injury and Regulate Gut Microbiota in Alcohol Liver Disease Mice Zhou, Jingxuan Zhang, Nanhai Zhao, Liang Wu, Wei Zhang, Liebing Zhou, Feng Li, Jingming Foods Article Astragalus, a medical and edible plant in China, shows several bioactive properties. However, the role of astragalus in attenuating alcoholic liver disease (ALD) is less clear. The objective of this project is to investigate the improving effect of astragalus saponins (AS) and astragalus polysaccharides (AP), which are the two primary constituents in astragalus on hepatic injury induced by alcohol, and the potential mechanisms of action. Different doses of AS (50 and 100 mg/kg bw) and AP (300 and 600 mg/kg bw) were orally given to alcohol-treated mice for four weeks. The results demonstrated that both AP and AS could reverse the increase of the levels of TC, TG, FFA, and LDL-C in serum, and the decrease of serum HDL-C content, as well as the elevation of hepatic TC and TG levels induced by alcohol. The activities of AST, ALT, ALP, and γ-GT in ALD mice were raised after AP and AS supplementation. The antioxidant markers (SOD, CAT, GSH, and GSH-Px) were obviously augmented and the pro-inflammatory cytokines (TNF-α, IL-6 and IL-1β) and hepatic histological variations were alleviated by AP and AS, which was in line with the levels of oxidative stress-associated genes (Keap1, Nfe2l2, Nqo1, and Hmox1) and inflammation-associated genes (Tlr4, Myd88 and Nfkb1). In addition, AS exerted a more efficient effect than AP and the results presented dose proportionality. Moreover, AS and AP could modulate the intestinal microbiota disturbance induced by alcohol. Overall, AS and AP administration could ameliorate lipid accumulation in the serum and liver, as well as hepatic function, oxidative stress, inflammatory response, and gut flora disorders in mice as a result of alcohol. MDPI 2021-11-03 /pmc/articles/PMC8623381/ /pubmed/34828972 http://dx.doi.org/10.3390/foods10112688 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhou, Jingxuan
Zhang, Nanhai
Zhao, Liang
Wu, Wei
Zhang, Liebing
Zhou, Feng
Li, Jingming
Astragalus Polysaccharides and Saponins Alleviate Liver Injury and Regulate Gut Microbiota in Alcohol Liver Disease Mice
title Astragalus Polysaccharides and Saponins Alleviate Liver Injury and Regulate Gut Microbiota in Alcohol Liver Disease Mice
title_full Astragalus Polysaccharides and Saponins Alleviate Liver Injury and Regulate Gut Microbiota in Alcohol Liver Disease Mice
title_fullStr Astragalus Polysaccharides and Saponins Alleviate Liver Injury and Regulate Gut Microbiota in Alcohol Liver Disease Mice
title_full_unstemmed Astragalus Polysaccharides and Saponins Alleviate Liver Injury and Regulate Gut Microbiota in Alcohol Liver Disease Mice
title_short Astragalus Polysaccharides and Saponins Alleviate Liver Injury and Regulate Gut Microbiota in Alcohol Liver Disease Mice
title_sort astragalus polysaccharides and saponins alleviate liver injury and regulate gut microbiota in alcohol liver disease mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8623381/
https://www.ncbi.nlm.nih.gov/pubmed/34828972
http://dx.doi.org/10.3390/foods10112688
work_keys_str_mv AT zhoujingxuan astragaluspolysaccharidesandsaponinsalleviateliverinjuryandregulategutmicrobiotainalcoholliverdiseasemice
AT zhangnanhai astragaluspolysaccharidesandsaponinsalleviateliverinjuryandregulategutmicrobiotainalcoholliverdiseasemice
AT zhaoliang astragaluspolysaccharidesandsaponinsalleviateliverinjuryandregulategutmicrobiotainalcoholliverdiseasemice
AT wuwei astragaluspolysaccharidesandsaponinsalleviateliverinjuryandregulategutmicrobiotainalcoholliverdiseasemice
AT zhangliebing astragaluspolysaccharidesandsaponinsalleviateliverinjuryandregulategutmicrobiotainalcoholliverdiseasemice
AT zhoufeng astragaluspolysaccharidesandsaponinsalleviateliverinjuryandregulategutmicrobiotainalcoholliverdiseasemice
AT lijingming astragaluspolysaccharidesandsaponinsalleviateliverinjuryandregulategutmicrobiotainalcoholliverdiseasemice