Cargando…

A Comparative Study of the Hepatoprotective Effect of Centella asiatica Extract (CA-HE50) on Lipopolysaccharide/d-galactosamine-Induced Acute Liver Injury in C57BL/6 Mice

Acute liver failure (ALF) refers to the sudden loss of liver function and is accompanied by several complications. In a previous study, we revealed the protective effect of Centella asiatica 50% ethanol extract (CA-HE50) on acetaminophen-induced liver injury. In the present study, we investigate the...

Descripción completa

Detalles Bibliográficos
Autores principales: Hong, Woojae, Hwang-Bo, Jeon, Jeon, Hyelin, Ko, Minsung, Choi, Joongyeon, Jeong, Yong-Joon, Park, Jae-Hyun, Kim, Inhye, Kim, Tae-Woo, Kim, Hyunggun, Kang, Se-Chan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8623393/
https://www.ncbi.nlm.nih.gov/pubmed/34836346
http://dx.doi.org/10.3390/nu13114090
_version_ 1784605922051489792
author Hong, Woojae
Hwang-Bo, Jeon
Jeon, Hyelin
Ko, Minsung
Choi, Joongyeon
Jeong, Yong-Joon
Park, Jae-Hyun
Kim, Inhye
Kim, Tae-Woo
Kim, Hyunggun
Kang, Se-Chan
author_facet Hong, Woojae
Hwang-Bo, Jeon
Jeon, Hyelin
Ko, Minsung
Choi, Joongyeon
Jeong, Yong-Joon
Park, Jae-Hyun
Kim, Inhye
Kim, Tae-Woo
Kim, Hyunggun
Kang, Se-Chan
author_sort Hong, Woojae
collection PubMed
description Acute liver failure (ALF) refers to the sudden loss of liver function and is accompanied by several complications. In a previous study, we revealed the protective effect of Centella asiatica 50% ethanol extract (CA-HE50) on acetaminophen-induced liver injury. In the present study, we investigate the hepatoprotective effect of CA-HE50 in a lipopolysaccharide/galactosamine (LPS-D-Gal)-induced ALF animal model and compare it to existing therapeutic silymarin, Lentinus edodes mycelia (LEM) extracts, ursodeoxycholic acid (UDCA) and dimethyl diphenyl bicarboxylate (DDB). Serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels were decreased in the CA-HE50, silymarin, LEM, UDCA and DDB groups compared to the vehicle control group. In particular, AST and ALT levels of the 200 mg/kg CA-HE50 group were significantly decreased compared to positive control groups. Lactate dehydrogenase (LDH) levels were significantly decreased in the CA-HE50, silymarin, LEM, UDCA and DDB groups compared to the vehicle control group and LDH levels of the 200 mg/kg CA-HE50 group were similar to those of the positive control groups. Superoxide dismutase (SOD) activity was significantly increased in the 100 mg/kg CA-HE50, LEM and UDCA groups compared to the vehicle control group and, in particular, the 100 mg/kg CA-HE50 group increased significantly compared to positive control groups. In addition, the histopathological lesion score was significantly decreased in the CA-HE50 and positive control groups compared with the vehicle control group and the histopathological lesion score of the 200 mg/kg CA-HE50 group was similar to that of the positive control groups. These results show that CA-HE50 has antioxidant and hepatoprotective effects at a level similar to that of silymarin, LEM, UDCA and DDB, which are known to have hepatoprotective effects; further, CA-HE50 has potential as a prophylactic and therapeutic agent in ALF.
format Online
Article
Text
id pubmed-8623393
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-86233932021-11-27 A Comparative Study of the Hepatoprotective Effect of Centella asiatica Extract (CA-HE50) on Lipopolysaccharide/d-galactosamine-Induced Acute Liver Injury in C57BL/6 Mice Hong, Woojae Hwang-Bo, Jeon Jeon, Hyelin Ko, Minsung Choi, Joongyeon Jeong, Yong-Joon Park, Jae-Hyun Kim, Inhye Kim, Tae-Woo Kim, Hyunggun Kang, Se-Chan Nutrients Article Acute liver failure (ALF) refers to the sudden loss of liver function and is accompanied by several complications. In a previous study, we revealed the protective effect of Centella asiatica 50% ethanol extract (CA-HE50) on acetaminophen-induced liver injury. In the present study, we investigate the hepatoprotective effect of CA-HE50 in a lipopolysaccharide/galactosamine (LPS-D-Gal)-induced ALF animal model and compare it to existing therapeutic silymarin, Lentinus edodes mycelia (LEM) extracts, ursodeoxycholic acid (UDCA) and dimethyl diphenyl bicarboxylate (DDB). Serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels were decreased in the CA-HE50, silymarin, LEM, UDCA and DDB groups compared to the vehicle control group. In particular, AST and ALT levels of the 200 mg/kg CA-HE50 group were significantly decreased compared to positive control groups. Lactate dehydrogenase (LDH) levels were significantly decreased in the CA-HE50, silymarin, LEM, UDCA and DDB groups compared to the vehicle control group and LDH levels of the 200 mg/kg CA-HE50 group were similar to those of the positive control groups. Superoxide dismutase (SOD) activity was significantly increased in the 100 mg/kg CA-HE50, LEM and UDCA groups compared to the vehicle control group and, in particular, the 100 mg/kg CA-HE50 group increased significantly compared to positive control groups. In addition, the histopathological lesion score was significantly decreased in the CA-HE50 and positive control groups compared with the vehicle control group and the histopathological lesion score of the 200 mg/kg CA-HE50 group was similar to that of the positive control groups. These results show that CA-HE50 has antioxidant and hepatoprotective effects at a level similar to that of silymarin, LEM, UDCA and DDB, which are known to have hepatoprotective effects; further, CA-HE50 has potential as a prophylactic and therapeutic agent in ALF. MDPI 2021-11-15 /pmc/articles/PMC8623393/ /pubmed/34836346 http://dx.doi.org/10.3390/nu13114090 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hong, Woojae
Hwang-Bo, Jeon
Jeon, Hyelin
Ko, Minsung
Choi, Joongyeon
Jeong, Yong-Joon
Park, Jae-Hyun
Kim, Inhye
Kim, Tae-Woo
Kim, Hyunggun
Kang, Se-Chan
A Comparative Study of the Hepatoprotective Effect of Centella asiatica Extract (CA-HE50) on Lipopolysaccharide/d-galactosamine-Induced Acute Liver Injury in C57BL/6 Mice
title A Comparative Study of the Hepatoprotective Effect of Centella asiatica Extract (CA-HE50) on Lipopolysaccharide/d-galactosamine-Induced Acute Liver Injury in C57BL/6 Mice
title_full A Comparative Study of the Hepatoprotective Effect of Centella asiatica Extract (CA-HE50) on Lipopolysaccharide/d-galactosamine-Induced Acute Liver Injury in C57BL/6 Mice
title_fullStr A Comparative Study of the Hepatoprotective Effect of Centella asiatica Extract (CA-HE50) on Lipopolysaccharide/d-galactosamine-Induced Acute Liver Injury in C57BL/6 Mice
title_full_unstemmed A Comparative Study of the Hepatoprotective Effect of Centella asiatica Extract (CA-HE50) on Lipopolysaccharide/d-galactosamine-Induced Acute Liver Injury in C57BL/6 Mice
title_short A Comparative Study of the Hepatoprotective Effect of Centella asiatica Extract (CA-HE50) on Lipopolysaccharide/d-galactosamine-Induced Acute Liver Injury in C57BL/6 Mice
title_sort comparative study of the hepatoprotective effect of centella asiatica extract (ca-he50) on lipopolysaccharide/d-galactosamine-induced acute liver injury in c57bl/6 mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8623393/
https://www.ncbi.nlm.nih.gov/pubmed/34836346
http://dx.doi.org/10.3390/nu13114090
work_keys_str_mv AT hongwoojae acomparativestudyofthehepatoprotectiveeffectofcentellaasiaticaextractcahe50onlipopolysaccharidedgalactosamineinducedacuteliverinjuryinc57bl6mice
AT hwangbojeon acomparativestudyofthehepatoprotectiveeffectofcentellaasiaticaextractcahe50onlipopolysaccharidedgalactosamineinducedacuteliverinjuryinc57bl6mice
AT jeonhyelin acomparativestudyofthehepatoprotectiveeffectofcentellaasiaticaextractcahe50onlipopolysaccharidedgalactosamineinducedacuteliverinjuryinc57bl6mice
AT kominsung acomparativestudyofthehepatoprotectiveeffectofcentellaasiaticaextractcahe50onlipopolysaccharidedgalactosamineinducedacuteliverinjuryinc57bl6mice
AT choijoongyeon acomparativestudyofthehepatoprotectiveeffectofcentellaasiaticaextractcahe50onlipopolysaccharidedgalactosamineinducedacuteliverinjuryinc57bl6mice
AT jeongyongjoon acomparativestudyofthehepatoprotectiveeffectofcentellaasiaticaextractcahe50onlipopolysaccharidedgalactosamineinducedacuteliverinjuryinc57bl6mice
AT parkjaehyun acomparativestudyofthehepatoprotectiveeffectofcentellaasiaticaextractcahe50onlipopolysaccharidedgalactosamineinducedacuteliverinjuryinc57bl6mice
AT kiminhye acomparativestudyofthehepatoprotectiveeffectofcentellaasiaticaextractcahe50onlipopolysaccharidedgalactosamineinducedacuteliverinjuryinc57bl6mice
AT kimtaewoo acomparativestudyofthehepatoprotectiveeffectofcentellaasiaticaextractcahe50onlipopolysaccharidedgalactosamineinducedacuteliverinjuryinc57bl6mice
AT kimhyunggun acomparativestudyofthehepatoprotectiveeffectofcentellaasiaticaextractcahe50onlipopolysaccharidedgalactosamineinducedacuteliverinjuryinc57bl6mice
AT kangsechan acomparativestudyofthehepatoprotectiveeffectofcentellaasiaticaextractcahe50onlipopolysaccharidedgalactosamineinducedacuteliverinjuryinc57bl6mice
AT hongwoojae comparativestudyofthehepatoprotectiveeffectofcentellaasiaticaextractcahe50onlipopolysaccharidedgalactosamineinducedacuteliverinjuryinc57bl6mice
AT hwangbojeon comparativestudyofthehepatoprotectiveeffectofcentellaasiaticaextractcahe50onlipopolysaccharidedgalactosamineinducedacuteliverinjuryinc57bl6mice
AT jeonhyelin comparativestudyofthehepatoprotectiveeffectofcentellaasiaticaextractcahe50onlipopolysaccharidedgalactosamineinducedacuteliverinjuryinc57bl6mice
AT kominsung comparativestudyofthehepatoprotectiveeffectofcentellaasiaticaextractcahe50onlipopolysaccharidedgalactosamineinducedacuteliverinjuryinc57bl6mice
AT choijoongyeon comparativestudyofthehepatoprotectiveeffectofcentellaasiaticaextractcahe50onlipopolysaccharidedgalactosamineinducedacuteliverinjuryinc57bl6mice
AT jeongyongjoon comparativestudyofthehepatoprotectiveeffectofcentellaasiaticaextractcahe50onlipopolysaccharidedgalactosamineinducedacuteliverinjuryinc57bl6mice
AT parkjaehyun comparativestudyofthehepatoprotectiveeffectofcentellaasiaticaextractcahe50onlipopolysaccharidedgalactosamineinducedacuteliverinjuryinc57bl6mice
AT kiminhye comparativestudyofthehepatoprotectiveeffectofcentellaasiaticaextractcahe50onlipopolysaccharidedgalactosamineinducedacuteliverinjuryinc57bl6mice
AT kimtaewoo comparativestudyofthehepatoprotectiveeffectofcentellaasiaticaextractcahe50onlipopolysaccharidedgalactosamineinducedacuteliverinjuryinc57bl6mice
AT kimhyunggun comparativestudyofthehepatoprotectiveeffectofcentellaasiaticaextractcahe50onlipopolysaccharidedgalactosamineinducedacuteliverinjuryinc57bl6mice
AT kangsechan comparativestudyofthehepatoprotectiveeffectofcentellaasiaticaextractcahe50onlipopolysaccharidedgalactosamineinducedacuteliverinjuryinc57bl6mice