Combined Effect of Different Flower Stem Features on the Visiting Frequency of the Generalist Ant Lasius niger: An Experimental Study

SIMPLE SUMMARY: Flowering plants usually attract insect pollinators by offering them nectar, pollen or other energetically valuable sources. To deter ants, which are unreliable pollinators and can act as nectar thieves, plants have developed different systems either inside the flowers or associated with the stems. The latter one, called greasy pole syndrome, is based on the combined effect of several stem features hampering the access of ants to the apically located flowers. In this study, we examined the effects of different flower stem features in the round-leaved Alexanders Smyrnium rotundifolium on the visiting frequency of the generalist ant species, the black garden ant Lasius niger. We conducted the experiments with ants running on dry wooden sticks mimicking four different types of stems. To attract ants, we placed a sweet sugar syrup droplet on a stick tip. Ants visited different types of stem-mimicking sticks with significantly different frequencies. The highest number of insects were registered on untreated stick samples, whereas the lowest visiting frequency was observed on sticks bearing cuff-like structures (serving as macroscopic physical barriers) covered with a nano/microparticle film, which caused the slipperiness of the surface. Thus, by combining macroscopic obstacles and slippery surfaces, plants can protect their flowers from undesirable crawling visitors such as ants. ABSTRACT: In order to understand the effects of the morphology and surface texture of flower stems in Smyrnium rotundifolium on the visiting frequency of generalist ants, we conducted experiments with Lasius niger ants running on dry wooden sticks mimicking different types of stems: (1) intact (grooved) sticks; (2) sticks painted with slaked (hydrated) lime (calcium carbonate coverage) imitating plant epicuticular wax coverage; (3) intact sticks with smooth polyester plate-shaped cuffs imitating upper leaves; and (4) intact sticks bearing cuffs painted with slaked lime. Ants were attracted by the sweet sugar syrup droplets placed on a stick tip, and the number of ants visiting the drops was counted. Our data showed significant differences in the visiting frequencies between the different types of stem-mimicking samples. The number of recorded ants progressively decreased in the following order of samples: intact sticks—painted sticks—sticks with intact cuffs—sticks with painted cuffs. These results clearly demonstrated that micro/nanoscopic surface coverages and macroscopic physical barriers, especially if combined, have a negative impact on the attractiveness of stems to ants. This study provides further evidence for the hypothesis that having a diversity of plant stems in the field, generalist ants prefer substrates where their locomotion is less hindered by obstacles and/or surface slipperiness.