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Results of Proficiency Testing for Trichinella in Poland, 2015–2019

Trichinellosis is a zoonotic meat-borne disease caused by the nematodes of the genus Trichinella. Meat containing live Trichinella larvae is a source of infection. The examination of meat for Trichinella was introduced in 1869, but the digestion method for this did not appear in Poland until the lat...

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Autores principales: Różycki, Mirosław, Korpysa-Dzirba, Weronika, Bełcik, Aneta, Bilska-Zając, Ewa, Karamon, Jacek, Sroka, Jacek, Zdybel, Jolanta, Cencek, Tomasz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8623675/
https://www.ncbi.nlm.nih.gov/pubmed/34830669
http://dx.doi.org/10.3390/jcm10225389
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author Różycki, Mirosław
Korpysa-Dzirba, Weronika
Bełcik, Aneta
Bilska-Zając, Ewa
Karamon, Jacek
Sroka, Jacek
Zdybel, Jolanta
Cencek, Tomasz
author_facet Różycki, Mirosław
Korpysa-Dzirba, Weronika
Bełcik, Aneta
Bilska-Zając, Ewa
Karamon, Jacek
Sroka, Jacek
Zdybel, Jolanta
Cencek, Tomasz
author_sort Różycki, Mirosław
collection PubMed
description Trichinellosis is a zoonotic meat-borne disease caused by the nematodes of the genus Trichinella. Meat containing live Trichinella larvae is a source of infection. The examination of meat for Trichinella was introduced in 1869, but the digestion method for this did not appear in Poland until the late 1970s. Nowadays, the meat of all food animals susceptible to Trichinella spp. is examined in the frame of official post mortem control with the digestion method. The majority of laboratories in Poland meet the requirements of the ISO/IEC 17025 Standard (352 field laboratories). Laboratory personnel participate in quality control programs. This paper presents the results of proficiency tests (PTs) organized within 2015–2019 in Poland. Over this period, the laboratories examined 7580 samples (contamination levels: zero, one, three, and five larvae). Each laboratory was provided with a set of samples (one negative and three positive). Over 95% of the samples were considered correct in qualitative assessments, though the results of the quantitative evaluations were slightly lower, with 89% of samples being considered correct. Based on a sample evaluation, 88% of laboratories passed the PT comparison. A slight decrease was observed in the examination of samples spiked with five larvae, and great progress was achieved in samples containing three larvae. Low levels of sample contamination are sought after in laboratories but may make evaluations difficult. For this reason, we must consider increasing the number of larvae added to the samples in the next PTs.
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spelling pubmed-86236752021-11-27 Results of Proficiency Testing for Trichinella in Poland, 2015–2019 Różycki, Mirosław Korpysa-Dzirba, Weronika Bełcik, Aneta Bilska-Zając, Ewa Karamon, Jacek Sroka, Jacek Zdybel, Jolanta Cencek, Tomasz J Clin Med Article Trichinellosis is a zoonotic meat-borne disease caused by the nematodes of the genus Trichinella. Meat containing live Trichinella larvae is a source of infection. The examination of meat for Trichinella was introduced in 1869, but the digestion method for this did not appear in Poland until the late 1970s. Nowadays, the meat of all food animals susceptible to Trichinella spp. is examined in the frame of official post mortem control with the digestion method. The majority of laboratories in Poland meet the requirements of the ISO/IEC 17025 Standard (352 field laboratories). Laboratory personnel participate in quality control programs. This paper presents the results of proficiency tests (PTs) organized within 2015–2019 in Poland. Over this period, the laboratories examined 7580 samples (contamination levels: zero, one, three, and five larvae). Each laboratory was provided with a set of samples (one negative and three positive). Over 95% of the samples were considered correct in qualitative assessments, though the results of the quantitative evaluations were slightly lower, with 89% of samples being considered correct. Based on a sample evaluation, 88% of laboratories passed the PT comparison. A slight decrease was observed in the examination of samples spiked with five larvae, and great progress was achieved in samples containing three larvae. Low levels of sample contamination are sought after in laboratories but may make evaluations difficult. For this reason, we must consider increasing the number of larvae added to the samples in the next PTs. MDPI 2021-11-18 /pmc/articles/PMC8623675/ /pubmed/34830669 http://dx.doi.org/10.3390/jcm10225389 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Różycki, Mirosław
Korpysa-Dzirba, Weronika
Bełcik, Aneta
Bilska-Zając, Ewa
Karamon, Jacek
Sroka, Jacek
Zdybel, Jolanta
Cencek, Tomasz
Results of Proficiency Testing for Trichinella in Poland, 2015–2019
title Results of Proficiency Testing for Trichinella in Poland, 2015–2019
title_full Results of Proficiency Testing for Trichinella in Poland, 2015–2019
title_fullStr Results of Proficiency Testing for Trichinella in Poland, 2015–2019
title_full_unstemmed Results of Proficiency Testing for Trichinella in Poland, 2015–2019
title_short Results of Proficiency Testing for Trichinella in Poland, 2015–2019
title_sort results of proficiency testing for trichinella in poland, 2015–2019
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8623675/
https://www.ncbi.nlm.nih.gov/pubmed/34830669
http://dx.doi.org/10.3390/jcm10225389
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