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Improved In Vivo Delivery of Small RNA Based on the Calcium Phosphate Method
In the past few years, we have demonstrated the efficacy of a nanoparticle system, super carbonate apatite (sCA), for the in vivo delivery of siRNA/miRNA. Intravenous injection of sCA loaded with small RNAs results in safe, high tumor delivery in mouse models. To further improve the efficiency of tu...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8623677/ https://www.ncbi.nlm.nih.gov/pubmed/34834512 http://dx.doi.org/10.3390/jpm11111160 |
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author | Wu, Xin Yokoyama, Yuhki Takahashi, Hidekazu Kouda, Shihori Yamamoto, Hiroyuki Wang, Jiaqi Morimoto, Yoshihiro Minami, Kazumasa Hata, Tsuyoshi Shamma, Awad Inoue, Akira Ohtsuka, Masahisa Shibata, Satoshi Kobayashi, Shogo Akai, Shuji Yamamoto, Hirofumi |
author_facet | Wu, Xin Yokoyama, Yuhki Takahashi, Hidekazu Kouda, Shihori Yamamoto, Hiroyuki Wang, Jiaqi Morimoto, Yoshihiro Minami, Kazumasa Hata, Tsuyoshi Shamma, Awad Inoue, Akira Ohtsuka, Masahisa Shibata, Satoshi Kobayashi, Shogo Akai, Shuji Yamamoto, Hirofumi |
author_sort | Wu, Xin |
collection | PubMed |
description | In the past few years, we have demonstrated the efficacy of a nanoparticle system, super carbonate apatite (sCA), for the in vivo delivery of siRNA/miRNA. Intravenous injection of sCA loaded with small RNAs results in safe, high tumor delivery in mouse models. To further improve the efficiency of tumor delivery and avoid liver toxicity, we successfully developed an inorganic nanoparticle device (iNaD) via high-frequency ultrasonic pulverization combined with PEG blending during the production of sCA. Compared to sCA loaded with 24 μg of miRNA, systemic administration of iNaD loaded with 0.75 μg of miRNA demonstrated similar delivery efficiency to mouse tumors with little accumulation in the liver. In the mouse therapeutic model, iNaD loaded with 3 μg of the tumor suppressor small RNA MIRTX resulted in an improved anti-tumor effect compared to sCA loaded with 24 μg. Our findings on the bio-distribution and therapeutic effect of iNaD provide new perspectives for future nanomedicine engineering. |
format | Online Article Text |
id | pubmed-8623677 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-86236772021-11-27 Improved In Vivo Delivery of Small RNA Based on the Calcium Phosphate Method Wu, Xin Yokoyama, Yuhki Takahashi, Hidekazu Kouda, Shihori Yamamoto, Hiroyuki Wang, Jiaqi Morimoto, Yoshihiro Minami, Kazumasa Hata, Tsuyoshi Shamma, Awad Inoue, Akira Ohtsuka, Masahisa Shibata, Satoshi Kobayashi, Shogo Akai, Shuji Yamamoto, Hirofumi J Pers Med Article In the past few years, we have demonstrated the efficacy of a nanoparticle system, super carbonate apatite (sCA), for the in vivo delivery of siRNA/miRNA. Intravenous injection of sCA loaded with small RNAs results in safe, high tumor delivery in mouse models. To further improve the efficiency of tumor delivery and avoid liver toxicity, we successfully developed an inorganic nanoparticle device (iNaD) via high-frequency ultrasonic pulverization combined with PEG blending during the production of sCA. Compared to sCA loaded with 24 μg of miRNA, systemic administration of iNaD loaded with 0.75 μg of miRNA demonstrated similar delivery efficiency to mouse tumors with little accumulation in the liver. In the mouse therapeutic model, iNaD loaded with 3 μg of the tumor suppressor small RNA MIRTX resulted in an improved anti-tumor effect compared to sCA loaded with 24 μg. Our findings on the bio-distribution and therapeutic effect of iNaD provide new perspectives for future nanomedicine engineering. MDPI 2021-11-08 /pmc/articles/PMC8623677/ /pubmed/34834512 http://dx.doi.org/10.3390/jpm11111160 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wu, Xin Yokoyama, Yuhki Takahashi, Hidekazu Kouda, Shihori Yamamoto, Hiroyuki Wang, Jiaqi Morimoto, Yoshihiro Minami, Kazumasa Hata, Tsuyoshi Shamma, Awad Inoue, Akira Ohtsuka, Masahisa Shibata, Satoshi Kobayashi, Shogo Akai, Shuji Yamamoto, Hirofumi Improved In Vivo Delivery of Small RNA Based on the Calcium Phosphate Method |
title | Improved In Vivo Delivery of Small RNA Based on the Calcium Phosphate Method |
title_full | Improved In Vivo Delivery of Small RNA Based on the Calcium Phosphate Method |
title_fullStr | Improved In Vivo Delivery of Small RNA Based on the Calcium Phosphate Method |
title_full_unstemmed | Improved In Vivo Delivery of Small RNA Based on the Calcium Phosphate Method |
title_short | Improved In Vivo Delivery of Small RNA Based on the Calcium Phosphate Method |
title_sort | improved in vivo delivery of small rna based on the calcium phosphate method |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8623677/ https://www.ncbi.nlm.nih.gov/pubmed/34834512 http://dx.doi.org/10.3390/jpm11111160 |
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