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Complete Lipooligosaccharide Structure from Pseudoalteromonas nigrifaciens Sq02-Rif(r) and Study of Its Immunomodulatory Activity

Lipopolysaccharides (LPS) are surface glycoconjugates embedded in the external leaflet of the outer membrane (OM) of the Gram-negative bacteria. They consist of three regions: lipid A, core oligosaccharide (OS), and O-specific polysaccharide or O-antigen. Lipid A is the glycolipid endotoxin domain t...

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Autores principales: Di Guida, Rossella, Casillo, Angela, Stellavato, Antonietta, Di Meo, Celeste, Kawai, Soichiro, Kawamoto, Jun, Ogawa, Takuya, Kurihara, Tatsuo, Schiraldi, Chiara, Corsaro, Maria Michela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8623723/
https://www.ncbi.nlm.nih.gov/pubmed/34822517
http://dx.doi.org/10.3390/md19110646
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author Di Guida, Rossella
Casillo, Angela
Stellavato, Antonietta
Di Meo, Celeste
Kawai, Soichiro
Kawamoto, Jun
Ogawa, Takuya
Kurihara, Tatsuo
Schiraldi, Chiara
Corsaro, Maria Michela
author_facet Di Guida, Rossella
Casillo, Angela
Stellavato, Antonietta
Di Meo, Celeste
Kawai, Soichiro
Kawamoto, Jun
Ogawa, Takuya
Kurihara, Tatsuo
Schiraldi, Chiara
Corsaro, Maria Michela
author_sort Di Guida, Rossella
collection PubMed
description Lipopolysaccharides (LPS) are surface glycoconjugates embedded in the external leaflet of the outer membrane (OM) of the Gram-negative bacteria. They consist of three regions: lipid A, core oligosaccharide (OS), and O-specific polysaccharide or O-antigen. Lipid A is the glycolipid endotoxin domain that anchors the LPS molecule to the OM, and therefore, its chemical structure is crucial in the maintenance of membrane integrity in the Gram-negative bacteria. In this paper, we reported the characterization of the lipid A and OS structures from Pseudoalteromonas nigrifaciens Sq02-Rif(r), which is a psychrotrophic Gram-negative bacterium isolated from the intestine of Seriola quinqueradiata. The immunomodulatory activity of both LPS and lipid A was also examined.
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spelling pubmed-86237232021-11-27 Complete Lipooligosaccharide Structure from Pseudoalteromonas nigrifaciens Sq02-Rif(r) and Study of Its Immunomodulatory Activity Di Guida, Rossella Casillo, Angela Stellavato, Antonietta Di Meo, Celeste Kawai, Soichiro Kawamoto, Jun Ogawa, Takuya Kurihara, Tatsuo Schiraldi, Chiara Corsaro, Maria Michela Mar Drugs Article Lipopolysaccharides (LPS) are surface glycoconjugates embedded in the external leaflet of the outer membrane (OM) of the Gram-negative bacteria. They consist of three regions: lipid A, core oligosaccharide (OS), and O-specific polysaccharide or O-antigen. Lipid A is the glycolipid endotoxin domain that anchors the LPS molecule to the OM, and therefore, its chemical structure is crucial in the maintenance of membrane integrity in the Gram-negative bacteria. In this paper, we reported the characterization of the lipid A and OS structures from Pseudoalteromonas nigrifaciens Sq02-Rif(r), which is a psychrotrophic Gram-negative bacterium isolated from the intestine of Seriola quinqueradiata. The immunomodulatory activity of both LPS and lipid A was also examined. MDPI 2021-11-19 /pmc/articles/PMC8623723/ /pubmed/34822517 http://dx.doi.org/10.3390/md19110646 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Di Guida, Rossella
Casillo, Angela
Stellavato, Antonietta
Di Meo, Celeste
Kawai, Soichiro
Kawamoto, Jun
Ogawa, Takuya
Kurihara, Tatsuo
Schiraldi, Chiara
Corsaro, Maria Michela
Complete Lipooligosaccharide Structure from Pseudoalteromonas nigrifaciens Sq02-Rif(r) and Study of Its Immunomodulatory Activity
title Complete Lipooligosaccharide Structure from Pseudoalteromonas nigrifaciens Sq02-Rif(r) and Study of Its Immunomodulatory Activity
title_full Complete Lipooligosaccharide Structure from Pseudoalteromonas nigrifaciens Sq02-Rif(r) and Study of Its Immunomodulatory Activity
title_fullStr Complete Lipooligosaccharide Structure from Pseudoalteromonas nigrifaciens Sq02-Rif(r) and Study of Its Immunomodulatory Activity
title_full_unstemmed Complete Lipooligosaccharide Structure from Pseudoalteromonas nigrifaciens Sq02-Rif(r) and Study of Its Immunomodulatory Activity
title_short Complete Lipooligosaccharide Structure from Pseudoalteromonas nigrifaciens Sq02-Rif(r) and Study of Its Immunomodulatory Activity
title_sort complete lipooligosaccharide structure from pseudoalteromonas nigrifaciens sq02-rif(r) and study of its immunomodulatory activity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8623723/
https://www.ncbi.nlm.nih.gov/pubmed/34822517
http://dx.doi.org/10.3390/md19110646
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