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Complete Lipooligosaccharide Structure from Pseudoalteromonas nigrifaciens Sq02-Rif(r) and Study of Its Immunomodulatory Activity
Lipopolysaccharides (LPS) are surface glycoconjugates embedded in the external leaflet of the outer membrane (OM) of the Gram-negative bacteria. They consist of three regions: lipid A, core oligosaccharide (OS), and O-specific polysaccharide or O-antigen. Lipid A is the glycolipid endotoxin domain t...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8623723/ https://www.ncbi.nlm.nih.gov/pubmed/34822517 http://dx.doi.org/10.3390/md19110646 |
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author | Di Guida, Rossella Casillo, Angela Stellavato, Antonietta Di Meo, Celeste Kawai, Soichiro Kawamoto, Jun Ogawa, Takuya Kurihara, Tatsuo Schiraldi, Chiara Corsaro, Maria Michela |
author_facet | Di Guida, Rossella Casillo, Angela Stellavato, Antonietta Di Meo, Celeste Kawai, Soichiro Kawamoto, Jun Ogawa, Takuya Kurihara, Tatsuo Schiraldi, Chiara Corsaro, Maria Michela |
author_sort | Di Guida, Rossella |
collection | PubMed |
description | Lipopolysaccharides (LPS) are surface glycoconjugates embedded in the external leaflet of the outer membrane (OM) of the Gram-negative bacteria. They consist of three regions: lipid A, core oligosaccharide (OS), and O-specific polysaccharide or O-antigen. Lipid A is the glycolipid endotoxin domain that anchors the LPS molecule to the OM, and therefore, its chemical structure is crucial in the maintenance of membrane integrity in the Gram-negative bacteria. In this paper, we reported the characterization of the lipid A and OS structures from Pseudoalteromonas nigrifaciens Sq02-Rif(r), which is a psychrotrophic Gram-negative bacterium isolated from the intestine of Seriola quinqueradiata. The immunomodulatory activity of both LPS and lipid A was also examined. |
format | Online Article Text |
id | pubmed-8623723 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-86237232021-11-27 Complete Lipooligosaccharide Structure from Pseudoalteromonas nigrifaciens Sq02-Rif(r) and Study of Its Immunomodulatory Activity Di Guida, Rossella Casillo, Angela Stellavato, Antonietta Di Meo, Celeste Kawai, Soichiro Kawamoto, Jun Ogawa, Takuya Kurihara, Tatsuo Schiraldi, Chiara Corsaro, Maria Michela Mar Drugs Article Lipopolysaccharides (LPS) are surface glycoconjugates embedded in the external leaflet of the outer membrane (OM) of the Gram-negative bacteria. They consist of three regions: lipid A, core oligosaccharide (OS), and O-specific polysaccharide or O-antigen. Lipid A is the glycolipid endotoxin domain that anchors the LPS molecule to the OM, and therefore, its chemical structure is crucial in the maintenance of membrane integrity in the Gram-negative bacteria. In this paper, we reported the characterization of the lipid A and OS structures from Pseudoalteromonas nigrifaciens Sq02-Rif(r), which is a psychrotrophic Gram-negative bacterium isolated from the intestine of Seriola quinqueradiata. The immunomodulatory activity of both LPS and lipid A was also examined. MDPI 2021-11-19 /pmc/articles/PMC8623723/ /pubmed/34822517 http://dx.doi.org/10.3390/md19110646 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Di Guida, Rossella Casillo, Angela Stellavato, Antonietta Di Meo, Celeste Kawai, Soichiro Kawamoto, Jun Ogawa, Takuya Kurihara, Tatsuo Schiraldi, Chiara Corsaro, Maria Michela Complete Lipooligosaccharide Structure from Pseudoalteromonas nigrifaciens Sq02-Rif(r) and Study of Its Immunomodulatory Activity |
title | Complete Lipooligosaccharide Structure from Pseudoalteromonas nigrifaciens Sq02-Rif(r) and Study of Its Immunomodulatory Activity |
title_full | Complete Lipooligosaccharide Structure from Pseudoalteromonas nigrifaciens Sq02-Rif(r) and Study of Its Immunomodulatory Activity |
title_fullStr | Complete Lipooligosaccharide Structure from Pseudoalteromonas nigrifaciens Sq02-Rif(r) and Study of Its Immunomodulatory Activity |
title_full_unstemmed | Complete Lipooligosaccharide Structure from Pseudoalteromonas nigrifaciens Sq02-Rif(r) and Study of Its Immunomodulatory Activity |
title_short | Complete Lipooligosaccharide Structure from Pseudoalteromonas nigrifaciens Sq02-Rif(r) and Study of Its Immunomodulatory Activity |
title_sort | complete lipooligosaccharide structure from pseudoalteromonas nigrifaciens sq02-rif(r) and study of its immunomodulatory activity |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8623723/ https://www.ncbi.nlm.nih.gov/pubmed/34822517 http://dx.doi.org/10.3390/md19110646 |
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