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Intestinal Cellular Biomarkers of Mucosal Lesion Progression in Pediatric Celiac Disease

Celiac disease (CD) is a chronic intestinal inflammation caused by gluten ingestion in genetically predisposed individuals. Overt-CD and potential-CD are the two main forms of gluten intolerance in pediatric patients with different grades of intestinal mucosa lesion and clinical management. For over...

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Autores principales: Vitale, Serena, Maglio, Mariantonia, Picascia, Stefania, Mottola, Ilaria, Miele, Erasmo, Troncone, Riccardo, Auricchio, Renata, Gianfrani, Carmen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8623763/
https://www.ncbi.nlm.nih.gov/pubmed/34834386
http://dx.doi.org/10.3390/pharmaceutics13111971
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author Vitale, Serena
Maglio, Mariantonia
Picascia, Stefania
Mottola, Ilaria
Miele, Erasmo
Troncone, Riccardo
Auricchio, Renata
Gianfrani, Carmen
author_facet Vitale, Serena
Maglio, Mariantonia
Picascia, Stefania
Mottola, Ilaria
Miele, Erasmo
Troncone, Riccardo
Auricchio, Renata
Gianfrani, Carmen
author_sort Vitale, Serena
collection PubMed
description Celiac disease (CD) is a chronic intestinal inflammation caused by gluten ingestion in genetically predisposed individuals. Overt-CD and potential-CD are the two main forms of gluten intolerance in pediatric patients with different grades of intestinal mucosa lesion and clinical management. For overt-CD patients the gluten-free diet is mandatory, while for potential-CD the dietary therapy is recommended only for those subjects becoming clinically symptomatic overtime. To date, specific early biomarkers of evolution to villous atrophy in potential-CD are lacking. We recently observed an expansion of TCRγδ+ T cells and a concomitant disappearance of IL4-producing T cells in the intestinal mucosa of overt-CD patients compared to potential-CD children, suggesting the involvement of these two cells subsets in the transition from potential-CD to overt-CD. In this study, we demonstrated that the intestinal densities of IL4+ T cells inversely correlated with TCRγδ+ T cell expansion (p < 0.005) and with the serum levels of anti-tissue transglutaminase antibodies (p < 0.01). The changes of these two cell subsets strongly correlated with mucosal lesions, according to the histological Marsh classification, as the transition from M0 to M3 lesions was associated with a significant reduction of IL4+ T cells (M0 vs. M1 p < 0.04, M0 vs. M3 p < 0.007) and an increase of TCRγδ+ T cells (M0 vs. M1 p < 0.05, M0 vs. M3 p < 0.0006). These findings strongly suggest that the detection of TCRγδ+ and IL4+ T cells could serve as cellular biomarkers of mucosal lesion and targets of novel immunomodulatory therapies for CD.
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spelling pubmed-86237632021-11-27 Intestinal Cellular Biomarkers of Mucosal Lesion Progression in Pediatric Celiac Disease Vitale, Serena Maglio, Mariantonia Picascia, Stefania Mottola, Ilaria Miele, Erasmo Troncone, Riccardo Auricchio, Renata Gianfrani, Carmen Pharmaceutics Article Celiac disease (CD) is a chronic intestinal inflammation caused by gluten ingestion in genetically predisposed individuals. Overt-CD and potential-CD are the two main forms of gluten intolerance in pediatric patients with different grades of intestinal mucosa lesion and clinical management. For overt-CD patients the gluten-free diet is mandatory, while for potential-CD the dietary therapy is recommended only for those subjects becoming clinically symptomatic overtime. To date, specific early biomarkers of evolution to villous atrophy in potential-CD are lacking. We recently observed an expansion of TCRγδ+ T cells and a concomitant disappearance of IL4-producing T cells in the intestinal mucosa of overt-CD patients compared to potential-CD children, suggesting the involvement of these two cells subsets in the transition from potential-CD to overt-CD. In this study, we demonstrated that the intestinal densities of IL4+ T cells inversely correlated with TCRγδ+ T cell expansion (p < 0.005) and with the serum levels of anti-tissue transglutaminase antibodies (p < 0.01). The changes of these two cell subsets strongly correlated with mucosal lesions, according to the histological Marsh classification, as the transition from M0 to M3 lesions was associated with a significant reduction of IL4+ T cells (M0 vs. M1 p < 0.04, M0 vs. M3 p < 0.007) and an increase of TCRγδ+ T cells (M0 vs. M1 p < 0.05, M0 vs. M3 p < 0.0006). These findings strongly suggest that the detection of TCRγδ+ and IL4+ T cells could serve as cellular biomarkers of mucosal lesion and targets of novel immunomodulatory therapies for CD. MDPI 2021-11-20 /pmc/articles/PMC8623763/ /pubmed/34834386 http://dx.doi.org/10.3390/pharmaceutics13111971 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Vitale, Serena
Maglio, Mariantonia
Picascia, Stefania
Mottola, Ilaria
Miele, Erasmo
Troncone, Riccardo
Auricchio, Renata
Gianfrani, Carmen
Intestinal Cellular Biomarkers of Mucosal Lesion Progression in Pediatric Celiac Disease
title Intestinal Cellular Biomarkers of Mucosal Lesion Progression in Pediatric Celiac Disease
title_full Intestinal Cellular Biomarkers of Mucosal Lesion Progression in Pediatric Celiac Disease
title_fullStr Intestinal Cellular Biomarkers of Mucosal Lesion Progression in Pediatric Celiac Disease
title_full_unstemmed Intestinal Cellular Biomarkers of Mucosal Lesion Progression in Pediatric Celiac Disease
title_short Intestinal Cellular Biomarkers of Mucosal Lesion Progression in Pediatric Celiac Disease
title_sort intestinal cellular biomarkers of mucosal lesion progression in pediatric celiac disease
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8623763/
https://www.ncbi.nlm.nih.gov/pubmed/34834386
http://dx.doi.org/10.3390/pharmaceutics13111971
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