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Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in a Dog in Connecticut in February 2021

We report the first detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in a 3-month-old dog in Connecticut that died suddenly and was submitted to the state veterinary diagnostic laboratory for postmortem examination. Viral RNA was detected in multiple organs of the dog by rev...

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Autores principales: Lee, Dong-Hun, Helal, Zeinab H., Kim, Junwon, Hunt, Amelia, Barbieri, Alyza, Tocco, Natalie, Frasca, Salvatore, Kerr, Kirklyn, Hyeon, Ji-Yeon, Chung, David H., Risatti, Guillermo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8623824/
https://www.ncbi.nlm.nih.gov/pubmed/34834948
http://dx.doi.org/10.3390/v13112141
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author Lee, Dong-Hun
Helal, Zeinab H.
Kim, Junwon
Hunt, Amelia
Barbieri, Alyza
Tocco, Natalie
Frasca, Salvatore
Kerr, Kirklyn
Hyeon, Ji-Yeon
Chung, David H.
Risatti, Guillermo
author_facet Lee, Dong-Hun
Helal, Zeinab H.
Kim, Junwon
Hunt, Amelia
Barbieri, Alyza
Tocco, Natalie
Frasca, Salvatore
Kerr, Kirklyn
Hyeon, Ji-Yeon
Chung, David H.
Risatti, Guillermo
author_sort Lee, Dong-Hun
collection PubMed
description We report the first detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in a 3-month-old dog in Connecticut that died suddenly and was submitted to the state veterinary diagnostic laboratory for postmortem examination. Viral RNA was detected in multiple organs of the dog by reverse transcription real time-PCR (RT-qPCR). Negative and positive sense strands of viral RNA were visualized by in situ hybridization using RNAscope technology. Complete genome sequencing and phylogenetic analysis of the hCoV-19/USA/CT-CVMDL-Dog-1/2021 (CT_Dog/2021) virus were conducted to identify the origin and lineage of the virus. The CT_Dog/2021 virus belonged to the GH/B1.2. genetic lineage and was genetically similar to SARS-CoV-2 identified in humans in the U.S. during the winter of 2020–2021. However, it was not related to other SARS-CoV-2 variants identified from companion animals in the U.S. It contained both the D614G in spike and P323L in nsp12 substitutions, which have become the dominant mutations in the United States. The continued sporadic detections of SARS-CoV-2 in companion animals warrant public health concerns about the zoonotic potential of SARS-CoV-2 and enhance our collective understanding of the epidemiology of the virus.
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spelling pubmed-86238242021-11-27 Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in a Dog in Connecticut in February 2021 Lee, Dong-Hun Helal, Zeinab H. Kim, Junwon Hunt, Amelia Barbieri, Alyza Tocco, Natalie Frasca, Salvatore Kerr, Kirklyn Hyeon, Ji-Yeon Chung, David H. Risatti, Guillermo Viruses Brief Report We report the first detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in a 3-month-old dog in Connecticut that died suddenly and was submitted to the state veterinary diagnostic laboratory for postmortem examination. Viral RNA was detected in multiple organs of the dog by reverse transcription real time-PCR (RT-qPCR). Negative and positive sense strands of viral RNA were visualized by in situ hybridization using RNAscope technology. Complete genome sequencing and phylogenetic analysis of the hCoV-19/USA/CT-CVMDL-Dog-1/2021 (CT_Dog/2021) virus were conducted to identify the origin and lineage of the virus. The CT_Dog/2021 virus belonged to the GH/B1.2. genetic lineage and was genetically similar to SARS-CoV-2 identified in humans in the U.S. during the winter of 2020–2021. However, it was not related to other SARS-CoV-2 variants identified from companion animals in the U.S. It contained both the D614G in spike and P323L in nsp12 substitutions, which have become the dominant mutations in the United States. The continued sporadic detections of SARS-CoV-2 in companion animals warrant public health concerns about the zoonotic potential of SARS-CoV-2 and enhance our collective understanding of the epidemiology of the virus. MDPI 2021-10-23 /pmc/articles/PMC8623824/ /pubmed/34834948 http://dx.doi.org/10.3390/v13112141 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Report
Lee, Dong-Hun
Helal, Zeinab H.
Kim, Junwon
Hunt, Amelia
Barbieri, Alyza
Tocco, Natalie
Frasca, Salvatore
Kerr, Kirklyn
Hyeon, Ji-Yeon
Chung, David H.
Risatti, Guillermo
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in a Dog in Connecticut in February 2021
title Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in a Dog in Connecticut in February 2021
title_full Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in a Dog in Connecticut in February 2021
title_fullStr Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in a Dog in Connecticut in February 2021
title_full_unstemmed Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in a Dog in Connecticut in February 2021
title_short Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in a Dog in Connecticut in February 2021
title_sort severe acute respiratory syndrome coronavirus 2 (sars-cov-2) in a dog in connecticut in february 2021
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8623824/
https://www.ncbi.nlm.nih.gov/pubmed/34834948
http://dx.doi.org/10.3390/v13112141
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