Improving Current Knowledge on Seroprevalence and Genetic Characterization of Swine Influenza Virus in Croatian Pig Farms: A Retrospective Study

In a total of 1536 blood serum samples analysed by ELISA, antibodies for IAV nucleoprotein (NP) were detected in 30.3%. Results from HI show that the most common subtype of swIAV in the Croatian pig population was H1N1 (44.6%), followed by H3N2 (42.7%) and H1N2 (26.3%). Antibodies to at least one su...

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Autores principales: Jungić, Andreja, Savić, Vladimir, Madić, Josip, Barbić, Ljubo, Roić, Besi, Brnić, Dragan, Prpić, Jelena, Jemeršić, Lorena, Novosel, Dinko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8623915/
https://www.ncbi.nlm.nih.gov/pubmed/34832682
http://dx.doi.org/10.3390/pathogens10111527
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author Jungić, Andreja
Savić, Vladimir
Madić, Josip
Barbić, Ljubo
Roić, Besi
Brnić, Dragan
Prpić, Jelena
Jemeršić, Lorena
Novosel, Dinko
author_facet Jungić, Andreja
Savić, Vladimir
Madić, Josip
Barbić, Ljubo
Roić, Besi
Brnić, Dragan
Prpić, Jelena
Jemeršić, Lorena
Novosel, Dinko
author_sort Jungić, Andreja
collection PubMed
description In a total of 1536 blood serum samples analysed by ELISA, antibodies for IAV nucleoprotein (NP) were detected in 30.3%. Results from HI show that the most common subtype of swIAV in the Croatian pig population was H1N1 (44.6%), followed by H3N2 (42.7%) and H1N2 (26.3%). Antibodies to at least one subtype were detected in 62.19% of blood serum samples. Detection of swIAV antigen was performed by IHC and detected in 8 of 28 lung samples collected post-mortem. The matrix (M) gene was detected in nine of one hundred and forty-two lung tissue samples and in seven of twenty-nine nasopharyngeal swabs. Phylogenetic analysis of amplified HA and NA gene fragments in Croatian isolates suggests the presence of swIAV H1avN1av.
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spelling pubmed-86239152021-11-27 Improving Current Knowledge on Seroprevalence and Genetic Characterization of Swine Influenza Virus in Croatian Pig Farms: A Retrospective Study Jungić, Andreja Savić, Vladimir Madić, Josip Barbić, Ljubo Roić, Besi Brnić, Dragan Prpić, Jelena Jemeršić, Lorena Novosel, Dinko Pathogens Article In a total of 1536 blood serum samples analysed by ELISA, antibodies for IAV nucleoprotein (NP) were detected in 30.3%. Results from HI show that the most common subtype of swIAV in the Croatian pig population was H1N1 (44.6%), followed by H3N2 (42.7%) and H1N2 (26.3%). Antibodies to at least one subtype were detected in 62.19% of blood serum samples. Detection of swIAV antigen was performed by IHC and detected in 8 of 28 lung samples collected post-mortem. The matrix (M) gene was detected in nine of one hundred and forty-two lung tissue samples and in seven of twenty-nine nasopharyngeal swabs. Phylogenetic analysis of amplified HA and NA gene fragments in Croatian isolates suggests the presence of swIAV H1avN1av. MDPI 2021-11-22 /pmc/articles/PMC8623915/ /pubmed/34832682 http://dx.doi.org/10.3390/pathogens10111527 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jungić, Andreja
Savić, Vladimir
Madić, Josip
Barbić, Ljubo
Roić, Besi
Brnić, Dragan
Prpić, Jelena
Jemeršić, Lorena
Novosel, Dinko
Improving Current Knowledge on Seroprevalence and Genetic Characterization of Swine Influenza Virus in Croatian Pig Farms: A Retrospective Study
title Improving Current Knowledge on Seroprevalence and Genetic Characterization of Swine Influenza Virus in Croatian Pig Farms: A Retrospective Study
title_full Improving Current Knowledge on Seroprevalence and Genetic Characterization of Swine Influenza Virus in Croatian Pig Farms: A Retrospective Study
title_fullStr Improving Current Knowledge on Seroprevalence and Genetic Characterization of Swine Influenza Virus in Croatian Pig Farms: A Retrospective Study
title_full_unstemmed Improving Current Knowledge on Seroprevalence and Genetic Characterization of Swine Influenza Virus in Croatian Pig Farms: A Retrospective Study
title_short Improving Current Knowledge on Seroprevalence and Genetic Characterization of Swine Influenza Virus in Croatian Pig Farms: A Retrospective Study
title_sort improving current knowledge on seroprevalence and genetic characterization of swine influenza virus in croatian pig farms: a retrospective study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8623915/
https://www.ncbi.nlm.nih.gov/pubmed/34832682
http://dx.doi.org/10.3390/pathogens10111527
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