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Unravel the Local Complexity of Biological Environments by MALDI Mass Spectrometry Imaging

Classic metabolomic methods have proven to be very useful to study functional biology and variation in the chemical composition of different tissues. However, they do not provide any information in terms of spatial localization within fine structures. Matrix-assisted laser desorption ionization mass...

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Detalles Bibliográficos
Autores principales: Sgobba, Elvira, Daguerre, Yohann, Giampà, Marco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8623934/
https://www.ncbi.nlm.nih.gov/pubmed/34830273
http://dx.doi.org/10.3390/ijms222212393
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author Sgobba, Elvira
Daguerre, Yohann
Giampà, Marco
author_facet Sgobba, Elvira
Daguerre, Yohann
Giampà, Marco
author_sort Sgobba, Elvira
collection PubMed
description Classic metabolomic methods have proven to be very useful to study functional biology and variation in the chemical composition of different tissues. However, they do not provide any information in terms of spatial localization within fine structures. Matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI MSI) does and reaches at best a spatial resolution of 0.25 μm depending on the laser setup, making it a very powerful tool to analyze the local complexity of biological samples at the cellular level. Here, we intend to give an overview of the diversity of the molecules and localizations analyzed using this method as well as to update on the latest adaptations made to circumvent the complexity of samples. MALDI MSI has been widely used in medical sciences and is now developing in research areas as diverse as entomology, microbiology, plant biology, and plant–microbe interactions, the rhizobia symbiosis being the most exhaustively described so far. Those are the fields of interest on which we will focus to demonstrate MALDI MSI strengths in characterizing the spatial distributions of metabolites, lipids, and peptides in relation to biological questions.
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spelling pubmed-86239342021-11-27 Unravel the Local Complexity of Biological Environments by MALDI Mass Spectrometry Imaging Sgobba, Elvira Daguerre, Yohann Giampà, Marco Int J Mol Sci Review Classic metabolomic methods have proven to be very useful to study functional biology and variation in the chemical composition of different tissues. However, they do not provide any information in terms of spatial localization within fine structures. Matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI MSI) does and reaches at best a spatial resolution of 0.25 μm depending on the laser setup, making it a very powerful tool to analyze the local complexity of biological samples at the cellular level. Here, we intend to give an overview of the diversity of the molecules and localizations analyzed using this method as well as to update on the latest adaptations made to circumvent the complexity of samples. MALDI MSI has been widely used in medical sciences and is now developing in research areas as diverse as entomology, microbiology, plant biology, and plant–microbe interactions, the rhizobia symbiosis being the most exhaustively described so far. Those are the fields of interest on which we will focus to demonstrate MALDI MSI strengths in characterizing the spatial distributions of metabolites, lipids, and peptides in relation to biological questions. MDPI 2021-11-17 /pmc/articles/PMC8623934/ /pubmed/34830273 http://dx.doi.org/10.3390/ijms222212393 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Sgobba, Elvira
Daguerre, Yohann
Giampà, Marco
Unravel the Local Complexity of Biological Environments by MALDI Mass Spectrometry Imaging
title Unravel the Local Complexity of Biological Environments by MALDI Mass Spectrometry Imaging
title_full Unravel the Local Complexity of Biological Environments by MALDI Mass Spectrometry Imaging
title_fullStr Unravel the Local Complexity of Biological Environments by MALDI Mass Spectrometry Imaging
title_full_unstemmed Unravel the Local Complexity of Biological Environments by MALDI Mass Spectrometry Imaging
title_short Unravel the Local Complexity of Biological Environments by MALDI Mass Spectrometry Imaging
title_sort unravel the local complexity of biological environments by maldi mass spectrometry imaging
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8623934/
https://www.ncbi.nlm.nih.gov/pubmed/34830273
http://dx.doi.org/10.3390/ijms222212393
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