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Target-Site Mutations and Expression of ALS Gene Copies Vary According to Echinochloa Species

The sustainability of rice cropping systems is jeopardized by the large number and variety of populations of polyploid Echinochloa spp. resistant to ALS inhibitors. Better knowledge of the Echinochloa species present in Italian rice fields and the study of ALS genes involved in target-site resistanc...

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Autores principales: Panozzo, Silvia, Mascanzoni, Elisa, Scarabel, Laura, Milani, Andrea, Dalazen, Giliardi, Merotto, Aldo J., Tranel, Patrick J., Sattin, Maurizio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8624184/
https://www.ncbi.nlm.nih.gov/pubmed/34828447
http://dx.doi.org/10.3390/genes12111841
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author Panozzo, Silvia
Mascanzoni, Elisa
Scarabel, Laura
Milani, Andrea
Dalazen, Giliardi
Merotto, Aldo J.
Tranel, Patrick J.
Sattin, Maurizio
author_facet Panozzo, Silvia
Mascanzoni, Elisa
Scarabel, Laura
Milani, Andrea
Dalazen, Giliardi
Merotto, Aldo J.
Tranel, Patrick J.
Sattin, Maurizio
author_sort Panozzo, Silvia
collection PubMed
description The sustainability of rice cropping systems is jeopardized by the large number and variety of populations of polyploid Echinochloa spp. resistant to ALS inhibitors. Better knowledge of the Echinochloa species present in Italian rice fields and the study of ALS genes involved in target-site resistance could significantly contribute to a better understanding of resistance evolution and management. Using a CAPS-rbcL molecular marker, two species, E. crus-galli (L.) P. Beauv. and E. oryzicola (Vasinger) Vasing., were identified as the most common species in rice in Italy. Mutations involved in ALS inhibitor resistance in the different species were identified and associated with the ALS homoeologs. The relative expression of the ALS gene copies was evaluated. Molecular characterization led to the identification of three ALS genes in E. crus-galli and two in E. oryzicola. The two species also carried different point mutations conferring resistance: Ala122Asn in E. crus-galli and Trp574Leu in E. oryzicola. Mutations were carried in the same gene copy (ALS1), which was significantly more expressed than the other copies (ALS2 and ALS3) in both species. These results explain the high resistance level of these populations and why mutations in the other ALS copies are not involved in herbicide resistance.
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spelling pubmed-86241842021-11-27 Target-Site Mutations and Expression of ALS Gene Copies Vary According to Echinochloa Species Panozzo, Silvia Mascanzoni, Elisa Scarabel, Laura Milani, Andrea Dalazen, Giliardi Merotto, Aldo J. Tranel, Patrick J. Sattin, Maurizio Genes (Basel) Article The sustainability of rice cropping systems is jeopardized by the large number and variety of populations of polyploid Echinochloa spp. resistant to ALS inhibitors. Better knowledge of the Echinochloa species present in Italian rice fields and the study of ALS genes involved in target-site resistance could significantly contribute to a better understanding of resistance evolution and management. Using a CAPS-rbcL molecular marker, two species, E. crus-galli (L.) P. Beauv. and E. oryzicola (Vasinger) Vasing., were identified as the most common species in rice in Italy. Mutations involved in ALS inhibitor resistance in the different species were identified and associated with the ALS homoeologs. The relative expression of the ALS gene copies was evaluated. Molecular characterization led to the identification of three ALS genes in E. crus-galli and two in E. oryzicola. The two species also carried different point mutations conferring resistance: Ala122Asn in E. crus-galli and Trp574Leu in E. oryzicola. Mutations were carried in the same gene copy (ALS1), which was significantly more expressed than the other copies (ALS2 and ALS3) in both species. These results explain the high resistance level of these populations and why mutations in the other ALS copies are not involved in herbicide resistance. MDPI 2021-11-22 /pmc/articles/PMC8624184/ /pubmed/34828447 http://dx.doi.org/10.3390/genes12111841 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Panozzo, Silvia
Mascanzoni, Elisa
Scarabel, Laura
Milani, Andrea
Dalazen, Giliardi
Merotto, Aldo J.
Tranel, Patrick J.
Sattin, Maurizio
Target-Site Mutations and Expression of ALS Gene Copies Vary According to Echinochloa Species
title Target-Site Mutations and Expression of ALS Gene Copies Vary According to Echinochloa Species
title_full Target-Site Mutations and Expression of ALS Gene Copies Vary According to Echinochloa Species
title_fullStr Target-Site Mutations and Expression of ALS Gene Copies Vary According to Echinochloa Species
title_full_unstemmed Target-Site Mutations and Expression of ALS Gene Copies Vary According to Echinochloa Species
title_short Target-Site Mutations and Expression of ALS Gene Copies Vary According to Echinochloa Species
title_sort target-site mutations and expression of als gene copies vary according to echinochloa species
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8624184/
https://www.ncbi.nlm.nih.gov/pubmed/34828447
http://dx.doi.org/10.3390/genes12111841
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