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Macrophages and Monocytes: “Trojan Horses” in COVID-19
We aimed to explore whether variants of SARS-CoV-2 (Chinese-derived strain (D614, lineage A), Italian strain PV10734 (D614G, lineage B.1.1) and Alpha strain (lineage B.1.1.7)) were able to infect monocytes (MN) and monocyte-derived macrophages (MDM) and whether these infected cells may, in turn, be...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8624282/ https://www.ncbi.nlm.nih.gov/pubmed/34834984 http://dx.doi.org/10.3390/v13112178 |
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author | Percivalle, Elena Sammartino, Josè Camilla Cassaniti, Irene Arbustini, Eloisa Urtis, Mario Smirnova, Alexandra Concardi, Monica Belgiovine, Cristina Ferrari, Alessandro Lilleri, Daniele Piralla, Antonio Baldanti, Fausto |
author_facet | Percivalle, Elena Sammartino, Josè Camilla Cassaniti, Irene Arbustini, Eloisa Urtis, Mario Smirnova, Alexandra Concardi, Monica Belgiovine, Cristina Ferrari, Alessandro Lilleri, Daniele Piralla, Antonio Baldanti, Fausto |
author_sort | Percivalle, Elena |
collection | PubMed |
description | We aimed to explore whether variants of SARS-CoV-2 (Chinese-derived strain (D614, lineage A), Italian strain PV10734 (D614G, lineage B.1.1) and Alpha strain (lineage B.1.1.7)) were able to infect monocytes (MN) and monocyte-derived macrophages (MDM) and whether these infected cells may, in turn, be vectors of infection. For this purpose, we designed an in vitro study following the evolution of MN and MDM infection at different time points in order to confirm whether these cells were permissive for SARS-CoV-2 replication. Finally, we investigated whether, regardless of viral replication, the persistent virus can be transferred to non-infected cells permissive for viral replication. Thus, we co-cultured the infected MN/MDM with permissive VERO E6 cells verifying the viral transmission. This is a further in vitro demonstration of the important role of MN and MDM in the dissemination of SARS-CoV-2 and evolution of the COVID-19 disease. |
format | Online Article Text |
id | pubmed-8624282 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-86242822021-11-27 Macrophages and Monocytes: “Trojan Horses” in COVID-19 Percivalle, Elena Sammartino, Josè Camilla Cassaniti, Irene Arbustini, Eloisa Urtis, Mario Smirnova, Alexandra Concardi, Monica Belgiovine, Cristina Ferrari, Alessandro Lilleri, Daniele Piralla, Antonio Baldanti, Fausto Viruses Article We aimed to explore whether variants of SARS-CoV-2 (Chinese-derived strain (D614, lineage A), Italian strain PV10734 (D614G, lineage B.1.1) and Alpha strain (lineage B.1.1.7)) were able to infect monocytes (MN) and monocyte-derived macrophages (MDM) and whether these infected cells may, in turn, be vectors of infection. For this purpose, we designed an in vitro study following the evolution of MN and MDM infection at different time points in order to confirm whether these cells were permissive for SARS-CoV-2 replication. Finally, we investigated whether, regardless of viral replication, the persistent virus can be transferred to non-infected cells permissive for viral replication. Thus, we co-cultured the infected MN/MDM with permissive VERO E6 cells verifying the viral transmission. This is a further in vitro demonstration of the important role of MN and MDM in the dissemination of SARS-CoV-2 and evolution of the COVID-19 disease. MDPI 2021-10-28 /pmc/articles/PMC8624282/ /pubmed/34834984 http://dx.doi.org/10.3390/v13112178 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Percivalle, Elena Sammartino, Josè Camilla Cassaniti, Irene Arbustini, Eloisa Urtis, Mario Smirnova, Alexandra Concardi, Monica Belgiovine, Cristina Ferrari, Alessandro Lilleri, Daniele Piralla, Antonio Baldanti, Fausto Macrophages and Monocytes: “Trojan Horses” in COVID-19 |
title | Macrophages and Monocytes: “Trojan Horses” in COVID-19 |
title_full | Macrophages and Monocytes: “Trojan Horses” in COVID-19 |
title_fullStr | Macrophages and Monocytes: “Trojan Horses” in COVID-19 |
title_full_unstemmed | Macrophages and Monocytes: “Trojan Horses” in COVID-19 |
title_short | Macrophages and Monocytes: “Trojan Horses” in COVID-19 |
title_sort | macrophages and monocytes: “trojan horses” in covid-19 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8624282/ https://www.ncbi.nlm.nih.gov/pubmed/34834984 http://dx.doi.org/10.3390/v13112178 |
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