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Macrophages and Monocytes: “Trojan Horses” in COVID-19

We aimed to explore whether variants of SARS-CoV-2 (Chinese-derived strain (D614, lineage A), Italian strain PV10734 (D614G, lineage B.1.1) and Alpha strain (lineage B.1.1.7)) were able to infect monocytes (MN) and monocyte-derived macrophages (MDM) and whether these infected cells may, in turn, be...

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Autores principales: Percivalle, Elena, Sammartino, Josè Camilla, Cassaniti, Irene, Arbustini, Eloisa, Urtis, Mario, Smirnova, Alexandra, Concardi, Monica, Belgiovine, Cristina, Ferrari, Alessandro, Lilleri, Daniele, Piralla, Antonio, Baldanti, Fausto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8624282/
https://www.ncbi.nlm.nih.gov/pubmed/34834984
http://dx.doi.org/10.3390/v13112178
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author Percivalle, Elena
Sammartino, Josè Camilla
Cassaniti, Irene
Arbustini, Eloisa
Urtis, Mario
Smirnova, Alexandra
Concardi, Monica
Belgiovine, Cristina
Ferrari, Alessandro
Lilleri, Daniele
Piralla, Antonio
Baldanti, Fausto
author_facet Percivalle, Elena
Sammartino, Josè Camilla
Cassaniti, Irene
Arbustini, Eloisa
Urtis, Mario
Smirnova, Alexandra
Concardi, Monica
Belgiovine, Cristina
Ferrari, Alessandro
Lilleri, Daniele
Piralla, Antonio
Baldanti, Fausto
author_sort Percivalle, Elena
collection PubMed
description We aimed to explore whether variants of SARS-CoV-2 (Chinese-derived strain (D614, lineage A), Italian strain PV10734 (D614G, lineage B.1.1) and Alpha strain (lineage B.1.1.7)) were able to infect monocytes (MN) and monocyte-derived macrophages (MDM) and whether these infected cells may, in turn, be vectors of infection. For this purpose, we designed an in vitro study following the evolution of MN and MDM infection at different time points in order to confirm whether these cells were permissive for SARS-CoV-2 replication. Finally, we investigated whether, regardless of viral replication, the persistent virus can be transferred to non-infected cells permissive for viral replication. Thus, we co-cultured the infected MN/MDM with permissive VERO E6 cells verifying the viral transmission. This is a further in vitro demonstration of the important role of MN and MDM in the dissemination of SARS-CoV-2 and evolution of the COVID-19 disease.
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spelling pubmed-86242822021-11-27 Macrophages and Monocytes: “Trojan Horses” in COVID-19 Percivalle, Elena Sammartino, Josè Camilla Cassaniti, Irene Arbustini, Eloisa Urtis, Mario Smirnova, Alexandra Concardi, Monica Belgiovine, Cristina Ferrari, Alessandro Lilleri, Daniele Piralla, Antonio Baldanti, Fausto Viruses Article We aimed to explore whether variants of SARS-CoV-2 (Chinese-derived strain (D614, lineage A), Italian strain PV10734 (D614G, lineage B.1.1) and Alpha strain (lineage B.1.1.7)) were able to infect monocytes (MN) and monocyte-derived macrophages (MDM) and whether these infected cells may, in turn, be vectors of infection. For this purpose, we designed an in vitro study following the evolution of MN and MDM infection at different time points in order to confirm whether these cells were permissive for SARS-CoV-2 replication. Finally, we investigated whether, regardless of viral replication, the persistent virus can be transferred to non-infected cells permissive for viral replication. Thus, we co-cultured the infected MN/MDM with permissive VERO E6 cells verifying the viral transmission. This is a further in vitro demonstration of the important role of MN and MDM in the dissemination of SARS-CoV-2 and evolution of the COVID-19 disease. MDPI 2021-10-28 /pmc/articles/PMC8624282/ /pubmed/34834984 http://dx.doi.org/10.3390/v13112178 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Percivalle, Elena
Sammartino, Josè Camilla
Cassaniti, Irene
Arbustini, Eloisa
Urtis, Mario
Smirnova, Alexandra
Concardi, Monica
Belgiovine, Cristina
Ferrari, Alessandro
Lilleri, Daniele
Piralla, Antonio
Baldanti, Fausto
Macrophages and Monocytes: “Trojan Horses” in COVID-19
title Macrophages and Monocytes: “Trojan Horses” in COVID-19
title_full Macrophages and Monocytes: “Trojan Horses” in COVID-19
title_fullStr Macrophages and Monocytes: “Trojan Horses” in COVID-19
title_full_unstemmed Macrophages and Monocytes: “Trojan Horses” in COVID-19
title_short Macrophages and Monocytes: “Trojan Horses” in COVID-19
title_sort macrophages and monocytes: “trojan horses” in covid-19
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8624282/
https://www.ncbi.nlm.nih.gov/pubmed/34834984
http://dx.doi.org/10.3390/v13112178
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