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Neurosurgical Approaches to Brain Tissue Harvesting for the Establishment of Cell Cultures in Neural Experimental Cell Models

In recent decades, cell biology has made rapid progress. Cell isolation and cultivation techniques, supported by modern laboratory procedures and experimental capabilities, provide a wide range of opportunities for in vitro research to study physiological and pathophysiological processes in health a...

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Autores principales: Gradišnik, Lidija, Bošnjak, Roman, Bunc, Gorazd, Ravnik, Janez, Maver, Tina, Velnar, Tomaž
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8624371/
https://www.ncbi.nlm.nih.gov/pubmed/34832259
http://dx.doi.org/10.3390/ma14226857
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author Gradišnik, Lidija
Bošnjak, Roman
Bunc, Gorazd
Ravnik, Janez
Maver, Tina
Velnar, Tomaž
author_facet Gradišnik, Lidija
Bošnjak, Roman
Bunc, Gorazd
Ravnik, Janez
Maver, Tina
Velnar, Tomaž
author_sort Gradišnik, Lidija
collection PubMed
description In recent decades, cell biology has made rapid progress. Cell isolation and cultivation techniques, supported by modern laboratory procedures and experimental capabilities, provide a wide range of opportunities for in vitro research to study physiological and pathophysiological processes in health and disease. They can also be used very efficiently for the analysis of biomaterials. Before a new biomaterial is ready for implantation into tissues and widespread use in clinical practice, it must be extensively tested. Experimental cell models, which are a suitable testing ground and the first line of empirical exploration of new biomaterials, must contain suitable cells that form the basis of biomaterial testing. To isolate a stable and suitable cell culture, many steps are required. The first and one of the most important steps is the collection of donor tissue, usually during a surgical procedure. Thus, the collection is the foundation for the success of cell isolation. This article explains the sources and neurosurgical procedures for obtaining brain tissue samples for cell isolation techniques, which are essential for biomaterial testing procedures.
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spelling pubmed-86243712021-11-27 Neurosurgical Approaches to Brain Tissue Harvesting for the Establishment of Cell Cultures in Neural Experimental Cell Models Gradišnik, Lidija Bošnjak, Roman Bunc, Gorazd Ravnik, Janez Maver, Tina Velnar, Tomaž Materials (Basel) Review In recent decades, cell biology has made rapid progress. Cell isolation and cultivation techniques, supported by modern laboratory procedures and experimental capabilities, provide a wide range of opportunities for in vitro research to study physiological and pathophysiological processes in health and disease. They can also be used very efficiently for the analysis of biomaterials. Before a new biomaterial is ready for implantation into tissues and widespread use in clinical practice, it must be extensively tested. Experimental cell models, which are a suitable testing ground and the first line of empirical exploration of new biomaterials, must contain suitable cells that form the basis of biomaterial testing. To isolate a stable and suitable cell culture, many steps are required. The first and one of the most important steps is the collection of donor tissue, usually during a surgical procedure. Thus, the collection is the foundation for the success of cell isolation. This article explains the sources and neurosurgical procedures for obtaining brain tissue samples for cell isolation techniques, which are essential for biomaterial testing procedures. MDPI 2021-11-13 /pmc/articles/PMC8624371/ /pubmed/34832259 http://dx.doi.org/10.3390/ma14226857 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Gradišnik, Lidija
Bošnjak, Roman
Bunc, Gorazd
Ravnik, Janez
Maver, Tina
Velnar, Tomaž
Neurosurgical Approaches to Brain Tissue Harvesting for the Establishment of Cell Cultures in Neural Experimental Cell Models
title Neurosurgical Approaches to Brain Tissue Harvesting for the Establishment of Cell Cultures in Neural Experimental Cell Models
title_full Neurosurgical Approaches to Brain Tissue Harvesting for the Establishment of Cell Cultures in Neural Experimental Cell Models
title_fullStr Neurosurgical Approaches to Brain Tissue Harvesting for the Establishment of Cell Cultures in Neural Experimental Cell Models
title_full_unstemmed Neurosurgical Approaches to Brain Tissue Harvesting for the Establishment of Cell Cultures in Neural Experimental Cell Models
title_short Neurosurgical Approaches to Brain Tissue Harvesting for the Establishment of Cell Cultures in Neural Experimental Cell Models
title_sort neurosurgical approaches to brain tissue harvesting for the establishment of cell cultures in neural experimental cell models
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8624371/
https://www.ncbi.nlm.nih.gov/pubmed/34832259
http://dx.doi.org/10.3390/ma14226857
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