CRISPR Tackles Emerging Viral Pathogens

Understanding the dynamic relationship between viral pathogens and cellular host factors is critical to furthering our knowledge of viral replication, disease mechanisms and development of anti-viral therapeutics. CRISPR genome editing technology has enhanced this understanding, by allowing identifi...

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Detalles Bibliográficos
Autores principales: Kirby, Emily N., Shue, Byron, Thomas, Paul Q., Beard, Michael R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8624524/
https://www.ncbi.nlm.nih.gov/pubmed/34834963
http://dx.doi.org/10.3390/v13112157
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author Kirby, Emily N.
Shue, Byron
Thomas, Paul Q.
Beard, Michael R.
author_facet Kirby, Emily N.
Shue, Byron
Thomas, Paul Q.
Beard, Michael R.
author_sort Kirby, Emily N.
collection PubMed
description Understanding the dynamic relationship between viral pathogens and cellular host factors is critical to furthering our knowledge of viral replication, disease mechanisms and development of anti-viral therapeutics. CRISPR genome editing technology has enhanced this understanding, by allowing identification of pro-viral and anti-viral cellular host factors for a wide range of viruses, most recently the cause of the COVID-19 pandemic, SARS-CoV-2. This review will discuss how CRISPR knockout and CRISPR activation genome-wide screening methods are a robust tool to investigate the viral life cycle and how other class 2 CRISPR systems are being repurposed for diagnostics.
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spelling pubmed-86245242021-11-27 CRISPR Tackles Emerging Viral Pathogens Kirby, Emily N. Shue, Byron Thomas, Paul Q. Beard, Michael R. Viruses Review Understanding the dynamic relationship between viral pathogens and cellular host factors is critical to furthering our knowledge of viral replication, disease mechanisms and development of anti-viral therapeutics. CRISPR genome editing technology has enhanced this understanding, by allowing identification of pro-viral and anti-viral cellular host factors for a wide range of viruses, most recently the cause of the COVID-19 pandemic, SARS-CoV-2. This review will discuss how CRISPR knockout and CRISPR activation genome-wide screening methods are a robust tool to investigate the viral life cycle and how other class 2 CRISPR systems are being repurposed for diagnostics. MDPI 2021-10-26 /pmc/articles/PMC8624524/ /pubmed/34834963 http://dx.doi.org/10.3390/v13112157 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Kirby, Emily N.
Shue, Byron
Thomas, Paul Q.
Beard, Michael R.
CRISPR Tackles Emerging Viral Pathogens
title CRISPR Tackles Emerging Viral Pathogens
title_full CRISPR Tackles Emerging Viral Pathogens
title_fullStr CRISPR Tackles Emerging Viral Pathogens
title_full_unstemmed CRISPR Tackles Emerging Viral Pathogens
title_short CRISPR Tackles Emerging Viral Pathogens
title_sort crispr tackles emerging viral pathogens
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8624524/
https://www.ncbi.nlm.nih.gov/pubmed/34834963
http://dx.doi.org/10.3390/v13112157
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