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Isolation, Culture and Comprehensive Characterization of Biological Properties of Human Urine-Derived Stem Cells

Mesenchymal stem cells (MSCs) represent an attractive source within the field of tissue engineering. However, their harvesting often requires invasive medical procedures. Urine-derived stem cells (UDSCs) display similar properties to MSCs, and their obtention and further processing is non-invasive f...

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Autores principales: Culenova, Martina, Nicodemou, Andreas, Novakova, Zuzana Varchulova, Debreova, Michaela, Smolinská, Veronika, Bernatova, Sona, Ivanisova, Dana, Novotna, Olga, Vasicek, Jaromir, Varga, Ivan, Ziaran, Stanislav, Danisovic, Lubos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8624597/
https://www.ncbi.nlm.nih.gov/pubmed/34830384
http://dx.doi.org/10.3390/ijms222212503
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author Culenova, Martina
Nicodemou, Andreas
Novakova, Zuzana Varchulova
Debreova, Michaela
Smolinská, Veronika
Bernatova, Sona
Ivanisova, Dana
Novotna, Olga
Vasicek, Jaromir
Varga, Ivan
Ziaran, Stanislav
Danisovic, Lubos
author_facet Culenova, Martina
Nicodemou, Andreas
Novakova, Zuzana Varchulova
Debreova, Michaela
Smolinská, Veronika
Bernatova, Sona
Ivanisova, Dana
Novotna, Olga
Vasicek, Jaromir
Varga, Ivan
Ziaran, Stanislav
Danisovic, Lubos
author_sort Culenova, Martina
collection PubMed
description Mesenchymal stem cells (MSCs) represent an attractive source within the field of tissue engineering. However, their harvesting often requires invasive medical procedures. Urine-derived stem cells (UDSCs) display similar properties to MSCs, and their obtention and further processing is non-invasive for the donors as well as low cost. Here, we offer a comprehensive analysis of their biological properties. The goal of this study was to analyze their morphology, stemness, differentiation potential and cytokine profile. We have successfully isolated UDSCs from 25 urine samples. First colonies emerged up to 9 days after the initial seeding. Cell doubling time was 45 ± 0.24 SD, and when seeded at the density of 100 cells/cm(2), they formed 42 ± 6.5 SD colonies within 10 days. Morphological analyzes revealed that two different types of the cell populations have been present. The first type had a rice-grain shape and the second one was characterized by a polyhedral shape. In several cell cultures, dome-shaped cells were observed as well. All examined UDSCs expressed typical MSC-like surface markers, CD73, CD90 and CD105. Moreover, conditioned media from UDSCs were harvested, and cytokine profile has been evaluated showing a significantly higher secretory rate of IL-8, IL-6 and chemokines MCP-1 and GM-CSF. We have also successfully induced human UDSCs into chondrogenic, osteogenic and myogenic cell lineages. Our findings indicate that UDSCs might have immense potential in the regeneration of the damaged tissues.
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spelling pubmed-86245972021-11-27 Isolation, Culture and Comprehensive Characterization of Biological Properties of Human Urine-Derived Stem Cells Culenova, Martina Nicodemou, Andreas Novakova, Zuzana Varchulova Debreova, Michaela Smolinská, Veronika Bernatova, Sona Ivanisova, Dana Novotna, Olga Vasicek, Jaromir Varga, Ivan Ziaran, Stanislav Danisovic, Lubos Int J Mol Sci Article Mesenchymal stem cells (MSCs) represent an attractive source within the field of tissue engineering. However, their harvesting often requires invasive medical procedures. Urine-derived stem cells (UDSCs) display similar properties to MSCs, and their obtention and further processing is non-invasive for the donors as well as low cost. Here, we offer a comprehensive analysis of their biological properties. The goal of this study was to analyze their morphology, stemness, differentiation potential and cytokine profile. We have successfully isolated UDSCs from 25 urine samples. First colonies emerged up to 9 days after the initial seeding. Cell doubling time was 45 ± 0.24 SD, and when seeded at the density of 100 cells/cm(2), they formed 42 ± 6.5 SD colonies within 10 days. Morphological analyzes revealed that two different types of the cell populations have been present. The first type had a rice-grain shape and the second one was characterized by a polyhedral shape. In several cell cultures, dome-shaped cells were observed as well. All examined UDSCs expressed typical MSC-like surface markers, CD73, CD90 and CD105. Moreover, conditioned media from UDSCs were harvested, and cytokine profile has been evaluated showing a significantly higher secretory rate of IL-8, IL-6 and chemokines MCP-1 and GM-CSF. We have also successfully induced human UDSCs into chondrogenic, osteogenic and myogenic cell lineages. Our findings indicate that UDSCs might have immense potential in the regeneration of the damaged tissues. MDPI 2021-11-19 /pmc/articles/PMC8624597/ /pubmed/34830384 http://dx.doi.org/10.3390/ijms222212503 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Culenova, Martina
Nicodemou, Andreas
Novakova, Zuzana Varchulova
Debreova, Michaela
Smolinská, Veronika
Bernatova, Sona
Ivanisova, Dana
Novotna, Olga
Vasicek, Jaromir
Varga, Ivan
Ziaran, Stanislav
Danisovic, Lubos
Isolation, Culture and Comprehensive Characterization of Biological Properties of Human Urine-Derived Stem Cells
title Isolation, Culture and Comprehensive Characterization of Biological Properties of Human Urine-Derived Stem Cells
title_full Isolation, Culture and Comprehensive Characterization of Biological Properties of Human Urine-Derived Stem Cells
title_fullStr Isolation, Culture and Comprehensive Characterization of Biological Properties of Human Urine-Derived Stem Cells
title_full_unstemmed Isolation, Culture and Comprehensive Characterization of Biological Properties of Human Urine-Derived Stem Cells
title_short Isolation, Culture and Comprehensive Characterization of Biological Properties of Human Urine-Derived Stem Cells
title_sort isolation, culture and comprehensive characterization of biological properties of human urine-derived stem cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8624597/
https://www.ncbi.nlm.nih.gov/pubmed/34830384
http://dx.doi.org/10.3390/ijms222212503
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