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Interaction between Macrophages and Human Mesenchymal Stromal Cells Derived from Bone Marrow and Wharton’s Jelly—A Comparative Study

Despite intensive clinical research on the use of mesenchymal stromal cells (MSCs), further basic research in this field is still required. Herein, we compared human bone marrow MSCs (BM-MSCs, n = 6) and Wharton’s jelly MSCs (WJ-MSCs, n = 6) in their ability to interact with human primary macrophage...

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Autores principales: Dymowska, Marta, Aksamit, Aleksandra, Zielniok, Katarzyna, Kniotek, Monika, Kaleta, Beata, Roszczyk, Aleksander, Zych, Michal, Dabrowski, Filip, Paczek, Leszek, Burdzinska, Anna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8624657/
https://www.ncbi.nlm.nih.gov/pubmed/34834238
http://dx.doi.org/10.3390/pharmaceutics13111822
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author Dymowska, Marta
Aksamit, Aleksandra
Zielniok, Katarzyna
Kniotek, Monika
Kaleta, Beata
Roszczyk, Aleksander
Zych, Michal
Dabrowski, Filip
Paczek, Leszek
Burdzinska, Anna
author_facet Dymowska, Marta
Aksamit, Aleksandra
Zielniok, Katarzyna
Kniotek, Monika
Kaleta, Beata
Roszczyk, Aleksander
Zych, Michal
Dabrowski, Filip
Paczek, Leszek
Burdzinska, Anna
author_sort Dymowska, Marta
collection PubMed
description Despite intensive clinical research on the use of mesenchymal stromal cells (MSCs), further basic research in this field is still required. Herein, we compared human bone marrow MSCs (BM-MSCs, n = 6) and Wharton’s jelly MSCs (WJ-MSCs, n = 6) in their ability to interact with human primary macrophages. Evaluation of secretory potential revealed that under pro-inflammatory stimulation, WJ-MSCs secreted significantly more IL-6 than BM-MSCs (2-fold). This difference did not translate into the effect of MSCs on macrophages: both types of MSCs significantly directed M1-like macrophages toward the M2 phenotype (based on CD206 expression) to a similar extent. This observation was consistent both in flow cytometry analysis and immunocytochemical assessment. The effect of MSCs on macrophages was sustained when IL-6 signaling was blocked with Tocilizumab. Macrophages, regardless of polarization status, enhanced chemotaxis of both BM-MSCs and WJ-MSCs (p < 0.01; trans-well assay), with WJ-MSCs being significantly more responsive to M1-derived chemotactic signals than BM-MSCs. Furthermore, WJ-MSCs increased their motility (scratch assay) when exposed to macrophage-conditioned medium while BM-MSCs did not. These results indicate that although both BM-MSCs and WJ-MSCs have the ability to reciprocally interact with macrophages, the source of MSCs could slightly but significantly modify the response under clinical settings.
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spelling pubmed-86246572021-11-27 Interaction between Macrophages and Human Mesenchymal Stromal Cells Derived from Bone Marrow and Wharton’s Jelly—A Comparative Study Dymowska, Marta Aksamit, Aleksandra Zielniok, Katarzyna Kniotek, Monika Kaleta, Beata Roszczyk, Aleksander Zych, Michal Dabrowski, Filip Paczek, Leszek Burdzinska, Anna Pharmaceutics Article Despite intensive clinical research on the use of mesenchymal stromal cells (MSCs), further basic research in this field is still required. Herein, we compared human bone marrow MSCs (BM-MSCs, n = 6) and Wharton’s jelly MSCs (WJ-MSCs, n = 6) in their ability to interact with human primary macrophages. Evaluation of secretory potential revealed that under pro-inflammatory stimulation, WJ-MSCs secreted significantly more IL-6 than BM-MSCs (2-fold). This difference did not translate into the effect of MSCs on macrophages: both types of MSCs significantly directed M1-like macrophages toward the M2 phenotype (based on CD206 expression) to a similar extent. This observation was consistent both in flow cytometry analysis and immunocytochemical assessment. The effect of MSCs on macrophages was sustained when IL-6 signaling was blocked with Tocilizumab. Macrophages, regardless of polarization status, enhanced chemotaxis of both BM-MSCs and WJ-MSCs (p < 0.01; trans-well assay), with WJ-MSCs being significantly more responsive to M1-derived chemotactic signals than BM-MSCs. Furthermore, WJ-MSCs increased their motility (scratch assay) when exposed to macrophage-conditioned medium while BM-MSCs did not. These results indicate that although both BM-MSCs and WJ-MSCs have the ability to reciprocally interact with macrophages, the source of MSCs could slightly but significantly modify the response under clinical settings. MDPI 2021-11-01 /pmc/articles/PMC8624657/ /pubmed/34834238 http://dx.doi.org/10.3390/pharmaceutics13111822 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dymowska, Marta
Aksamit, Aleksandra
Zielniok, Katarzyna
Kniotek, Monika
Kaleta, Beata
Roszczyk, Aleksander
Zych, Michal
Dabrowski, Filip
Paczek, Leszek
Burdzinska, Anna
Interaction between Macrophages and Human Mesenchymal Stromal Cells Derived from Bone Marrow and Wharton’s Jelly—A Comparative Study
title Interaction between Macrophages and Human Mesenchymal Stromal Cells Derived from Bone Marrow and Wharton’s Jelly—A Comparative Study
title_full Interaction between Macrophages and Human Mesenchymal Stromal Cells Derived from Bone Marrow and Wharton’s Jelly—A Comparative Study
title_fullStr Interaction between Macrophages and Human Mesenchymal Stromal Cells Derived from Bone Marrow and Wharton’s Jelly—A Comparative Study
title_full_unstemmed Interaction between Macrophages and Human Mesenchymal Stromal Cells Derived from Bone Marrow and Wharton’s Jelly—A Comparative Study
title_short Interaction between Macrophages and Human Mesenchymal Stromal Cells Derived from Bone Marrow and Wharton’s Jelly—A Comparative Study
title_sort interaction between macrophages and human mesenchymal stromal cells derived from bone marrow and wharton’s jelly—a comparative study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8624657/
https://www.ncbi.nlm.nih.gov/pubmed/34834238
http://dx.doi.org/10.3390/pharmaceutics13111822
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