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Babesia microti Immunoreactive Rhoptry-Associated Protein-1 Paralogs Are Ancestral Members of the Piroplasmid-Confined RAP-1 Family

Babesia, Cytauxzoon and Theileria are tick-borne apicomplexan parasites of the order Piroplasmida, responsible for diseases in humans and animals. Members of the piroplasmid rhoptry-associated protein-1 (pRAP-1) family have a signature cysteine-rich domain and are important for parasite development....

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Autores principales: Bastos, Reginaldo G., Thekkiniath, Jose, Ben Mamoun, Choukri, Fuller, Lee, Molestina, Robert E., Florin-Christensen, Monica, Schnittger, Leonhard, Alzan, Heba F., Suarez, Carlos E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8624774/
https://www.ncbi.nlm.nih.gov/pubmed/34832541
http://dx.doi.org/10.3390/pathogens10111384
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author Bastos, Reginaldo G.
Thekkiniath, Jose
Ben Mamoun, Choukri
Fuller, Lee
Molestina, Robert E.
Florin-Christensen, Monica
Schnittger, Leonhard
Alzan, Heba F.
Suarez, Carlos E.
author_facet Bastos, Reginaldo G.
Thekkiniath, Jose
Ben Mamoun, Choukri
Fuller, Lee
Molestina, Robert E.
Florin-Christensen, Monica
Schnittger, Leonhard
Alzan, Heba F.
Suarez, Carlos E.
author_sort Bastos, Reginaldo G.
collection PubMed
description Babesia, Cytauxzoon and Theileria are tick-borne apicomplexan parasites of the order Piroplasmida, responsible for diseases in humans and animals. Members of the piroplasmid rhoptry-associated protein-1 (pRAP-1) family have a signature cysteine-rich domain and are important for parasite development. We propose that the closely linked B. microti genes annotated as BMR1_03g00947 and BMR1_03g00960 encode two paralogue pRAP-1-like proteins named BmIPA48 and Bm960. The two genes are tandemly arranged head to tail, highly expressed in blood stage parasites, syntenic to rap-1 genes of other piroplasmids, and share large portions of an almost identical ~225 bp sequence located in their 5′ putative regulatory regions. BmIPA48 and Bm960 proteins contain a N-terminal signal peptide, share very low sequence identity (<13%) with pRAP-1 from other species, and harbor one or more transmembrane domains. Diversification of the piroplasmid-confined prap-1 family is characterized by amplification of genes, protein domains, and a high sequence polymorphism. This suggests a functional involvement of pRAP-1 at the parasite-host interface, possibly in parasite adhesion, attachment, and/or evasion of the host immune defenses. Both BmIPA48 and Bm960 are recognized by antibodies in sera from humans infected with B. microti and might be promising candidates for developing novel serodiagnosis and vaccines.
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spelling pubmed-86247742021-11-27 Babesia microti Immunoreactive Rhoptry-Associated Protein-1 Paralogs Are Ancestral Members of the Piroplasmid-Confined RAP-1 Family Bastos, Reginaldo G. Thekkiniath, Jose Ben Mamoun, Choukri Fuller, Lee Molestina, Robert E. Florin-Christensen, Monica Schnittger, Leonhard Alzan, Heba F. Suarez, Carlos E. Pathogens Article Babesia, Cytauxzoon and Theileria are tick-borne apicomplexan parasites of the order Piroplasmida, responsible for diseases in humans and animals. Members of the piroplasmid rhoptry-associated protein-1 (pRAP-1) family have a signature cysteine-rich domain and are important for parasite development. We propose that the closely linked B. microti genes annotated as BMR1_03g00947 and BMR1_03g00960 encode two paralogue pRAP-1-like proteins named BmIPA48 and Bm960. The two genes are tandemly arranged head to tail, highly expressed in blood stage parasites, syntenic to rap-1 genes of other piroplasmids, and share large portions of an almost identical ~225 bp sequence located in their 5′ putative regulatory regions. BmIPA48 and Bm960 proteins contain a N-terminal signal peptide, share very low sequence identity (<13%) with pRAP-1 from other species, and harbor one or more transmembrane domains. Diversification of the piroplasmid-confined prap-1 family is characterized by amplification of genes, protein domains, and a high sequence polymorphism. This suggests a functional involvement of pRAP-1 at the parasite-host interface, possibly in parasite adhesion, attachment, and/or evasion of the host immune defenses. Both BmIPA48 and Bm960 are recognized by antibodies in sera from humans infected with B. microti and might be promising candidates for developing novel serodiagnosis and vaccines. MDPI 2021-10-26 /pmc/articles/PMC8624774/ /pubmed/34832541 http://dx.doi.org/10.3390/pathogens10111384 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bastos, Reginaldo G.
Thekkiniath, Jose
Ben Mamoun, Choukri
Fuller, Lee
Molestina, Robert E.
Florin-Christensen, Monica
Schnittger, Leonhard
Alzan, Heba F.
Suarez, Carlos E.
Babesia microti Immunoreactive Rhoptry-Associated Protein-1 Paralogs Are Ancestral Members of the Piroplasmid-Confined RAP-1 Family
title Babesia microti Immunoreactive Rhoptry-Associated Protein-1 Paralogs Are Ancestral Members of the Piroplasmid-Confined RAP-1 Family
title_full Babesia microti Immunoreactive Rhoptry-Associated Protein-1 Paralogs Are Ancestral Members of the Piroplasmid-Confined RAP-1 Family
title_fullStr Babesia microti Immunoreactive Rhoptry-Associated Protein-1 Paralogs Are Ancestral Members of the Piroplasmid-Confined RAP-1 Family
title_full_unstemmed Babesia microti Immunoreactive Rhoptry-Associated Protein-1 Paralogs Are Ancestral Members of the Piroplasmid-Confined RAP-1 Family
title_short Babesia microti Immunoreactive Rhoptry-Associated Protein-1 Paralogs Are Ancestral Members of the Piroplasmid-Confined RAP-1 Family
title_sort babesia microti immunoreactive rhoptry-associated protein-1 paralogs are ancestral members of the piroplasmid-confined rap-1 family
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8624774/
https://www.ncbi.nlm.nih.gov/pubmed/34832541
http://dx.doi.org/10.3390/pathogens10111384
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