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Evaluation of Marrubium vulgare Growing Wild in Tunisia for Its Potential as a Dietary Supplement

Marrubium vulgare L., known as horehound, is a widespread and widely known plant that is used in beer breweries and also as a traditional remedy in Tunisia. In this study, methanolic extracts of plants harvested from five different locations were investigated for their antioxidant activities using t...

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Autores principales: Rezgui, Marwa, Basma, Mabrouk, Neng, Nuno, Nogueira, José Manuel, Bettaieb Ben-Kaab, Leila, Machado Araújo, Maria Eduarda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8624851/
https://www.ncbi.nlm.nih.gov/pubmed/34829146
http://dx.doi.org/10.3390/foods10112864
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author Rezgui, Marwa
Basma, Mabrouk
Neng, Nuno
Nogueira, José Manuel
Bettaieb Ben-Kaab, Leila
Machado Araújo, Maria Eduarda
author_facet Rezgui, Marwa
Basma, Mabrouk
Neng, Nuno
Nogueira, José Manuel
Bettaieb Ben-Kaab, Leila
Machado Araújo, Maria Eduarda
author_sort Rezgui, Marwa
collection PubMed
description Marrubium vulgare L., known as horehound, is a widespread and widely known plant that is used in beer breweries and also as a traditional remedy in Tunisia. In this study, methanolic extracts of plants harvested from five different locations were investigated for their antioxidant activities using three assays (ferric reducing power, radical scavenging activity, and β-carotene-linoleic acid bleaching assay) as well as the total phenolic content. The mineral composition of the plant was also investigated concerning the following elements: Fe, Mg, Ca, Cu, Zn, Mn, K, and three heavy metals, Ni, Pb, and Cd. Marrubiin, the major bioactive diterpenoid lactone, was quantified by NMR in the samples. The essential oils were obtained by hydrodistillation, and their radical scavenging activity was investigated. The toxicity of essential oils was evaluated against Artemia salina (the brine shrimp larva). The essential oil showed a weak radical scavenging activity and low toxicity. Data obtained from the five different locations showed that the antioxidant activity, as well as the total phenolic and marrubiin content, were strongly affected by the harvest sites. The metal content in the samples showed differences with the harvest location, but there was always a great abundance of calcium, magnesium, and potassium.
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spelling pubmed-86248512021-11-27 Evaluation of Marrubium vulgare Growing Wild in Tunisia for Its Potential as a Dietary Supplement Rezgui, Marwa Basma, Mabrouk Neng, Nuno Nogueira, José Manuel Bettaieb Ben-Kaab, Leila Machado Araújo, Maria Eduarda Foods Article Marrubium vulgare L., known as horehound, is a widespread and widely known plant that is used in beer breweries and also as a traditional remedy in Tunisia. In this study, methanolic extracts of plants harvested from five different locations were investigated for their antioxidant activities using three assays (ferric reducing power, radical scavenging activity, and β-carotene-linoleic acid bleaching assay) as well as the total phenolic content. The mineral composition of the plant was also investigated concerning the following elements: Fe, Mg, Ca, Cu, Zn, Mn, K, and three heavy metals, Ni, Pb, and Cd. Marrubiin, the major bioactive diterpenoid lactone, was quantified by NMR in the samples. The essential oils were obtained by hydrodistillation, and their radical scavenging activity was investigated. The toxicity of essential oils was evaluated against Artemia salina (the brine shrimp larva). The essential oil showed a weak radical scavenging activity and low toxicity. Data obtained from the five different locations showed that the antioxidant activity, as well as the total phenolic and marrubiin content, were strongly affected by the harvest sites. The metal content in the samples showed differences with the harvest location, but there was always a great abundance of calcium, magnesium, and potassium. MDPI 2021-11-19 /pmc/articles/PMC8624851/ /pubmed/34829146 http://dx.doi.org/10.3390/foods10112864 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rezgui, Marwa
Basma, Mabrouk
Neng, Nuno
Nogueira, José Manuel
Bettaieb Ben-Kaab, Leila
Machado Araújo, Maria Eduarda
Evaluation of Marrubium vulgare Growing Wild in Tunisia for Its Potential as a Dietary Supplement
title Evaluation of Marrubium vulgare Growing Wild in Tunisia for Its Potential as a Dietary Supplement
title_full Evaluation of Marrubium vulgare Growing Wild in Tunisia for Its Potential as a Dietary Supplement
title_fullStr Evaluation of Marrubium vulgare Growing Wild in Tunisia for Its Potential as a Dietary Supplement
title_full_unstemmed Evaluation of Marrubium vulgare Growing Wild in Tunisia for Its Potential as a Dietary Supplement
title_short Evaluation of Marrubium vulgare Growing Wild in Tunisia for Its Potential as a Dietary Supplement
title_sort evaluation of marrubium vulgare growing wild in tunisia for its potential as a dietary supplement
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8624851/
https://www.ncbi.nlm.nih.gov/pubmed/34829146
http://dx.doi.org/10.3390/foods10112864
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