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Seed Paternity Analysis Using SSR Markers to Assess Successful Pollen Donors in Mixed Olive Orchards

The olive tree (Olea europaea L.) is a wind-pollinated crop that exhibits an extreme alternate bearing habit. To improve fruit set, several methods have been used to determine the most successful compatible combinations of cultivars. In this study, priority is given to seed paternity analysis based...

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Autores principales: Vuletin Selak, Gabriela, Baruca Arbeiter, Alenka, Cuevas, Julián, Perica, Slavko, Pujic, Petar, Raboteg Božiković, Marina, Bandelj, Dunja
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8624852/
https://www.ncbi.nlm.nih.gov/pubmed/34834719
http://dx.doi.org/10.3390/plants10112356
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author Vuletin Selak, Gabriela
Baruca Arbeiter, Alenka
Cuevas, Julián
Perica, Slavko
Pujic, Petar
Raboteg Božiković, Marina
Bandelj, Dunja
author_facet Vuletin Selak, Gabriela
Baruca Arbeiter, Alenka
Cuevas, Julián
Perica, Slavko
Pujic, Petar
Raboteg Božiković, Marina
Bandelj, Dunja
author_sort Vuletin Selak, Gabriela
collection PubMed
description The olive tree (Olea europaea L.) is a wind-pollinated crop that exhibits an extreme alternate bearing habit. To improve fruit set, several methods have been used to determine the most successful compatible combinations of cultivars. In this study, priority is given to seed paternity analysis based on simple sequence repeats (SSRs), microsatellite markers used for the identification of potential pollen donors of cultivar ‘Oblica’ in a mixed olive orchard during two consecutive years. Seven microsatellite primers were successfully used to examine the paternity of olive embryos from ‘Oblica’ mother trees. Embryos were considered as a product of self-fertilization if only maternal alleles were present, but not a single case of self-fertilization was found among all the embryos analyzed. Two dominant pollen donors were not the closest nor the cultivars with the highest number of trees in the orchard, suggesting that cross-compatibility may have a key role in determining pollen donor success. In our earlier studies, pollen tube growth and fertilization success correlated with fruit set when controlled crosses between cultivars were performed; however, some discrepancy might appear compared to paternity analyses when mother trees have a free choice among different pollen sources from cultivars growing in their surroundings.
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spelling pubmed-86248522021-11-27 Seed Paternity Analysis Using SSR Markers to Assess Successful Pollen Donors in Mixed Olive Orchards Vuletin Selak, Gabriela Baruca Arbeiter, Alenka Cuevas, Julián Perica, Slavko Pujic, Petar Raboteg Božiković, Marina Bandelj, Dunja Plants (Basel) Article The olive tree (Olea europaea L.) is a wind-pollinated crop that exhibits an extreme alternate bearing habit. To improve fruit set, several methods have been used to determine the most successful compatible combinations of cultivars. In this study, priority is given to seed paternity analysis based on simple sequence repeats (SSRs), microsatellite markers used for the identification of potential pollen donors of cultivar ‘Oblica’ in a mixed olive orchard during two consecutive years. Seven microsatellite primers were successfully used to examine the paternity of olive embryos from ‘Oblica’ mother trees. Embryos were considered as a product of self-fertilization if only maternal alleles were present, but not a single case of self-fertilization was found among all the embryos analyzed. Two dominant pollen donors were not the closest nor the cultivars with the highest number of trees in the orchard, suggesting that cross-compatibility may have a key role in determining pollen donor success. In our earlier studies, pollen tube growth and fertilization success correlated with fruit set when controlled crosses between cultivars were performed; however, some discrepancy might appear compared to paternity analyses when mother trees have a free choice among different pollen sources from cultivars growing in their surroundings. MDPI 2021-10-31 /pmc/articles/PMC8624852/ /pubmed/34834719 http://dx.doi.org/10.3390/plants10112356 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Vuletin Selak, Gabriela
Baruca Arbeiter, Alenka
Cuevas, Julián
Perica, Slavko
Pujic, Petar
Raboteg Božiković, Marina
Bandelj, Dunja
Seed Paternity Analysis Using SSR Markers to Assess Successful Pollen Donors in Mixed Olive Orchards
title Seed Paternity Analysis Using SSR Markers to Assess Successful Pollen Donors in Mixed Olive Orchards
title_full Seed Paternity Analysis Using SSR Markers to Assess Successful Pollen Donors in Mixed Olive Orchards
title_fullStr Seed Paternity Analysis Using SSR Markers to Assess Successful Pollen Donors in Mixed Olive Orchards
title_full_unstemmed Seed Paternity Analysis Using SSR Markers to Assess Successful Pollen Donors in Mixed Olive Orchards
title_short Seed Paternity Analysis Using SSR Markers to Assess Successful Pollen Donors in Mixed Olive Orchards
title_sort seed paternity analysis using ssr markers to assess successful pollen donors in mixed olive orchards
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8624852/
https://www.ncbi.nlm.nih.gov/pubmed/34834719
http://dx.doi.org/10.3390/plants10112356
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