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Three New Phthalide Glycosides from the Rhizomes of Cnidium officinale and Their Recovery Effect on Damaged Otic Hair Cells in Zebrafish

The extract from Cnidium officinale rhizomes was shown in a prior experiment to markedly recover otic hair cells in zebrafish damaged by neomycin. The current study was brought about to identify the principal metabolite. Column chromatography using octadecyl SiO(2) and SiO(2) was performed to isolat...

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Autores principales: Kim, Hyoung-Geun, Oh, Seon Min, Kim, Na Woo, Shim, Ji Heon, Nam, Youn Hee, Nguyen, Trong Nguyen, Lee, Min-Ho, Lee, Dae Young, Kang, Tong Ho, Baek, Nam-In
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8624857/
https://www.ncbi.nlm.nih.gov/pubmed/34834125
http://dx.doi.org/10.3390/molecules26227034
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author Kim, Hyoung-Geun
Oh, Seon Min
Kim, Na Woo
Shim, Ji Heon
Nam, Youn Hee
Nguyen, Trong Nguyen
Lee, Min-Ho
Lee, Dae Young
Kang, Tong Ho
Baek, Nam-In
author_facet Kim, Hyoung-Geun
Oh, Seon Min
Kim, Na Woo
Shim, Ji Heon
Nam, Youn Hee
Nguyen, Trong Nguyen
Lee, Min-Ho
Lee, Dae Young
Kang, Tong Ho
Baek, Nam-In
author_sort Kim, Hyoung-Geun
collection PubMed
description The extract from Cnidium officinale rhizomes was shown in a prior experiment to markedly recover otic hair cells in zebrafish damaged by neomycin. The current study was brought about to identify the principal metabolite. Column chromatography using octadecyl SiO(2) and SiO(2) was performed to isolate the major metabolites from the active fraction. The chemical structures were resolved on the basis of spectroscopic data, including NMR, IR, MS, and circular dichroism (CD) data. The isolated phthalide glycosides were assessed for their recovery effect on damaged otic hair cells in neomycin-treated zebrafish. Three new phthalide glycosides were isolated, and their chemical structures, including stereochemical characteristics, were determined. Two glycosides (0.1 μM) showed a recovery effect (p < 0.01) on otic hair cells in zebrafish affected by neomycin ototoxicity. Repeated column chromatography led to the isolation of three new phthalide glycosides, named ligusticosides C (1), D (2), and E (3). Ligusticoside C and ligusticoside E recovered damaged otic hair cells in zebrafish.
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spelling pubmed-86248572021-11-27 Three New Phthalide Glycosides from the Rhizomes of Cnidium officinale and Their Recovery Effect on Damaged Otic Hair Cells in Zebrafish Kim, Hyoung-Geun Oh, Seon Min Kim, Na Woo Shim, Ji Heon Nam, Youn Hee Nguyen, Trong Nguyen Lee, Min-Ho Lee, Dae Young Kang, Tong Ho Baek, Nam-In Molecules Article The extract from Cnidium officinale rhizomes was shown in a prior experiment to markedly recover otic hair cells in zebrafish damaged by neomycin. The current study was brought about to identify the principal metabolite. Column chromatography using octadecyl SiO(2) and SiO(2) was performed to isolate the major metabolites from the active fraction. The chemical structures were resolved on the basis of spectroscopic data, including NMR, IR, MS, and circular dichroism (CD) data. The isolated phthalide glycosides were assessed for their recovery effect on damaged otic hair cells in neomycin-treated zebrafish. Three new phthalide glycosides were isolated, and their chemical structures, including stereochemical characteristics, were determined. Two glycosides (0.1 μM) showed a recovery effect (p < 0.01) on otic hair cells in zebrafish affected by neomycin ototoxicity. Repeated column chromatography led to the isolation of three new phthalide glycosides, named ligusticosides C (1), D (2), and E (3). Ligusticoside C and ligusticoside E recovered damaged otic hair cells in zebrafish. MDPI 2021-11-21 /pmc/articles/PMC8624857/ /pubmed/34834125 http://dx.doi.org/10.3390/molecules26227034 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kim, Hyoung-Geun
Oh, Seon Min
Kim, Na Woo
Shim, Ji Heon
Nam, Youn Hee
Nguyen, Trong Nguyen
Lee, Min-Ho
Lee, Dae Young
Kang, Tong Ho
Baek, Nam-In
Three New Phthalide Glycosides from the Rhizomes of Cnidium officinale and Their Recovery Effect on Damaged Otic Hair Cells in Zebrafish
title Three New Phthalide Glycosides from the Rhizomes of Cnidium officinale and Their Recovery Effect on Damaged Otic Hair Cells in Zebrafish
title_full Three New Phthalide Glycosides from the Rhizomes of Cnidium officinale and Their Recovery Effect on Damaged Otic Hair Cells in Zebrafish
title_fullStr Three New Phthalide Glycosides from the Rhizomes of Cnidium officinale and Their Recovery Effect on Damaged Otic Hair Cells in Zebrafish
title_full_unstemmed Three New Phthalide Glycosides from the Rhizomes of Cnidium officinale and Their Recovery Effect on Damaged Otic Hair Cells in Zebrafish
title_short Three New Phthalide Glycosides from the Rhizomes of Cnidium officinale and Their Recovery Effect on Damaged Otic Hair Cells in Zebrafish
title_sort three new phthalide glycosides from the rhizomes of cnidium officinale and their recovery effect on damaged otic hair cells in zebrafish
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8624857/
https://www.ncbi.nlm.nih.gov/pubmed/34834125
http://dx.doi.org/10.3390/molecules26227034
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