Cargando…

Genome-Wide Identification of GDSL-Type Esterase/Lipase Gene Family in Dasypyrum villosum L. Reveals That DvGELP53 Is Related to BSMV Infection

GDSL-type esterase/lipase proteins (GELPs) characterized by a conserved GDSL motif at their N-terminus belong to the lipid hydrolysis enzyme superfamily. In plants, GELPs play an important role in plant growth, development and stress response. The studies of the identification and characterization o...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Heng, Zhang, Xu, Zhao, Jia, Sun, Li, Wang, Haiyan, Zhu, Ying, Xiao, Jin, Wang, Xiue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8624868/
https://www.ncbi.nlm.nih.gov/pubmed/34830200
http://dx.doi.org/10.3390/ijms222212317
_version_ 1784606279334887424
author Zhang, Heng
Zhang, Xu
Zhao, Jia
Sun, Li
Wang, Haiyan
Zhu, Ying
Xiao, Jin
Wang, Xiue
author_facet Zhang, Heng
Zhang, Xu
Zhao, Jia
Sun, Li
Wang, Haiyan
Zhu, Ying
Xiao, Jin
Wang, Xiue
author_sort Zhang, Heng
collection PubMed
description GDSL-type esterase/lipase proteins (GELPs) characterized by a conserved GDSL motif at their N-terminus belong to the lipid hydrolysis enzyme superfamily. In plants, GELPs play an important role in plant growth, development and stress response. The studies of the identification and characterization of the GELP gene family in Triticeae have not been reported. In this study, 193 DvGELPs were identified in Dasypyrum villosum and classified into 11 groups (clade A–K) by means of phylogenetic analysis. Most DvGELPs contain only one GDSL domain, only four DvGELPs contain other domains besides the GDSL domain. Gene structure analysis indicated 35.2% DvGELP genes have four introns and five exons. In the promoter regions of the identified DvGELPs, we detected 4502 putative cis-elements, which were associated with plant hormones, plant growth, environmental stress and light responsiveness. Expression profiling revealed 36, 44 and 17 DvGELPs were highly expressed in the spike, the root and the grain, respectively. Further investigation of a root-specific expressing GELP, DvGELP53, indicated it was induced by a variety of biotic and abiotic stresses. The knockdown of DvGELP53 inhibited long-distance movement of BSMV in the tissue of D. villosum. This research provides a genome-wide glimpse of the D. villosum GELP genes and hints at the participation of DvGELP53 in the interaction between virus and plants.
format Online
Article
Text
id pubmed-8624868
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-86248682021-11-27 Genome-Wide Identification of GDSL-Type Esterase/Lipase Gene Family in Dasypyrum villosum L. Reveals That DvGELP53 Is Related to BSMV Infection Zhang, Heng Zhang, Xu Zhao, Jia Sun, Li Wang, Haiyan Zhu, Ying Xiao, Jin Wang, Xiue Int J Mol Sci Article GDSL-type esterase/lipase proteins (GELPs) characterized by a conserved GDSL motif at their N-terminus belong to the lipid hydrolysis enzyme superfamily. In plants, GELPs play an important role in plant growth, development and stress response. The studies of the identification and characterization of the GELP gene family in Triticeae have not been reported. In this study, 193 DvGELPs were identified in Dasypyrum villosum and classified into 11 groups (clade A–K) by means of phylogenetic analysis. Most DvGELPs contain only one GDSL domain, only four DvGELPs contain other domains besides the GDSL domain. Gene structure analysis indicated 35.2% DvGELP genes have four introns and five exons. In the promoter regions of the identified DvGELPs, we detected 4502 putative cis-elements, which were associated with plant hormones, plant growth, environmental stress and light responsiveness. Expression profiling revealed 36, 44 and 17 DvGELPs were highly expressed in the spike, the root and the grain, respectively. Further investigation of a root-specific expressing GELP, DvGELP53, indicated it was induced by a variety of biotic and abiotic stresses. The knockdown of DvGELP53 inhibited long-distance movement of BSMV in the tissue of D. villosum. This research provides a genome-wide glimpse of the D. villosum GELP genes and hints at the participation of DvGELP53 in the interaction between virus and plants. MDPI 2021-11-15 /pmc/articles/PMC8624868/ /pubmed/34830200 http://dx.doi.org/10.3390/ijms222212317 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Heng
Zhang, Xu
Zhao, Jia
Sun, Li
Wang, Haiyan
Zhu, Ying
Xiao, Jin
Wang, Xiue
Genome-Wide Identification of GDSL-Type Esterase/Lipase Gene Family in Dasypyrum villosum L. Reveals That DvGELP53 Is Related to BSMV Infection
title Genome-Wide Identification of GDSL-Type Esterase/Lipase Gene Family in Dasypyrum villosum L. Reveals That DvGELP53 Is Related to BSMV Infection
title_full Genome-Wide Identification of GDSL-Type Esterase/Lipase Gene Family in Dasypyrum villosum L. Reveals That DvGELP53 Is Related to BSMV Infection
title_fullStr Genome-Wide Identification of GDSL-Type Esterase/Lipase Gene Family in Dasypyrum villosum L. Reveals That DvGELP53 Is Related to BSMV Infection
title_full_unstemmed Genome-Wide Identification of GDSL-Type Esterase/Lipase Gene Family in Dasypyrum villosum L. Reveals That DvGELP53 Is Related to BSMV Infection
title_short Genome-Wide Identification of GDSL-Type Esterase/Lipase Gene Family in Dasypyrum villosum L. Reveals That DvGELP53 Is Related to BSMV Infection
title_sort genome-wide identification of gdsl-type esterase/lipase gene family in dasypyrum villosum l. reveals that dvgelp53 is related to bsmv infection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8624868/
https://www.ncbi.nlm.nih.gov/pubmed/34830200
http://dx.doi.org/10.3390/ijms222212317
work_keys_str_mv AT zhangheng genomewideidentificationofgdsltypeesteraselipasegenefamilyindasypyrumvillosumlrevealsthatdvgelp53isrelatedtobsmvinfection
AT zhangxu genomewideidentificationofgdsltypeesteraselipasegenefamilyindasypyrumvillosumlrevealsthatdvgelp53isrelatedtobsmvinfection
AT zhaojia genomewideidentificationofgdsltypeesteraselipasegenefamilyindasypyrumvillosumlrevealsthatdvgelp53isrelatedtobsmvinfection
AT sunli genomewideidentificationofgdsltypeesteraselipasegenefamilyindasypyrumvillosumlrevealsthatdvgelp53isrelatedtobsmvinfection
AT wanghaiyan genomewideidentificationofgdsltypeesteraselipasegenefamilyindasypyrumvillosumlrevealsthatdvgelp53isrelatedtobsmvinfection
AT zhuying genomewideidentificationofgdsltypeesteraselipasegenefamilyindasypyrumvillosumlrevealsthatdvgelp53isrelatedtobsmvinfection
AT xiaojin genomewideidentificationofgdsltypeesteraselipasegenefamilyindasypyrumvillosumlrevealsthatdvgelp53isrelatedtobsmvinfection
AT wangxiue genomewideidentificationofgdsltypeesteraselipasegenefamilyindasypyrumvillosumlrevealsthatdvgelp53isrelatedtobsmvinfection