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A Lipid-Coated Nanoconstruct Composed of Gold Nanoparticles Noncovalently Coated with Small Interfering RNA: Preparation, Purification and Characterization

There is an urgent need to develop systems for nucleic acid delivery, especially for the creation of effective therapeutics against various diseases. We have previously shown the feasibility of efficient delivery of small interfering RNA by means of gold nanoparticle-based multilayer nanoconstructs...

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Autores principales: Epanchintseva, Anna V., Poletaeva, Julia E., Dovydenko, Ilya S., Chelobanov, Boris P., Pyshnyi, Dmitrii V., Ryabchikova, Elena I., Pyshnaya, Inna A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8624966/
https://www.ncbi.nlm.nih.gov/pubmed/34835540
http://dx.doi.org/10.3390/nano11112775
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author Epanchintseva, Anna V.
Poletaeva, Julia E.
Dovydenko, Ilya S.
Chelobanov, Boris P.
Pyshnyi, Dmitrii V.
Ryabchikova, Elena I.
Pyshnaya, Inna A.
author_facet Epanchintseva, Anna V.
Poletaeva, Julia E.
Dovydenko, Ilya S.
Chelobanov, Boris P.
Pyshnyi, Dmitrii V.
Ryabchikova, Elena I.
Pyshnaya, Inna A.
author_sort Epanchintseva, Anna V.
collection PubMed
description There is an urgent need to develop systems for nucleic acid delivery, especially for the creation of effective therapeutics against various diseases. We have previously shown the feasibility of efficient delivery of small interfering RNA by means of gold nanoparticle-based multilayer nanoconstructs (MLNCs) for suppressing reporter protein synthesis. The present work is aimed at improving the quality of preparations of desired MLNCs, and for this purpose, optimal conditions for their multistep fabrication were found. All steps of this process and MLNC purification were verified using dynamic light scattering, transmission electron microscopy, and UV-Vis spectroscopy. Factors influencing the efficiency of nanocomposite assembly, colloidal stability, and purification quality were identified. These data made it possible to optimize the fabrication of target MLNCs bearing small interfering RNA and to substantially improve end product quality via an increase in its homogeneity and a decrease in the amount of incomplete nanoconstructs. We believe that the proposed approaches and methods will be useful for researchers working with lipid nanoconstructs.
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spelling pubmed-86249662021-11-27 A Lipid-Coated Nanoconstruct Composed of Gold Nanoparticles Noncovalently Coated with Small Interfering RNA: Preparation, Purification and Characterization Epanchintseva, Anna V. Poletaeva, Julia E. Dovydenko, Ilya S. Chelobanov, Boris P. Pyshnyi, Dmitrii V. Ryabchikova, Elena I. Pyshnaya, Inna A. Nanomaterials (Basel) Article There is an urgent need to develop systems for nucleic acid delivery, especially for the creation of effective therapeutics against various diseases. We have previously shown the feasibility of efficient delivery of small interfering RNA by means of gold nanoparticle-based multilayer nanoconstructs (MLNCs) for suppressing reporter protein synthesis. The present work is aimed at improving the quality of preparations of desired MLNCs, and for this purpose, optimal conditions for their multistep fabrication were found. All steps of this process and MLNC purification were verified using dynamic light scattering, transmission electron microscopy, and UV-Vis spectroscopy. Factors influencing the efficiency of nanocomposite assembly, colloidal stability, and purification quality were identified. These data made it possible to optimize the fabrication of target MLNCs bearing small interfering RNA and to substantially improve end product quality via an increase in its homogeneity and a decrease in the amount of incomplete nanoconstructs. We believe that the proposed approaches and methods will be useful for researchers working with lipid nanoconstructs. MDPI 2021-10-20 /pmc/articles/PMC8624966/ /pubmed/34835540 http://dx.doi.org/10.3390/nano11112775 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Epanchintseva, Anna V.
Poletaeva, Julia E.
Dovydenko, Ilya S.
Chelobanov, Boris P.
Pyshnyi, Dmitrii V.
Ryabchikova, Elena I.
Pyshnaya, Inna A.
A Lipid-Coated Nanoconstruct Composed of Gold Nanoparticles Noncovalently Coated with Small Interfering RNA: Preparation, Purification and Characterization
title A Lipid-Coated Nanoconstruct Composed of Gold Nanoparticles Noncovalently Coated with Small Interfering RNA: Preparation, Purification and Characterization
title_full A Lipid-Coated Nanoconstruct Composed of Gold Nanoparticles Noncovalently Coated with Small Interfering RNA: Preparation, Purification and Characterization
title_fullStr A Lipid-Coated Nanoconstruct Composed of Gold Nanoparticles Noncovalently Coated with Small Interfering RNA: Preparation, Purification and Characterization
title_full_unstemmed A Lipid-Coated Nanoconstruct Composed of Gold Nanoparticles Noncovalently Coated with Small Interfering RNA: Preparation, Purification and Characterization
title_short A Lipid-Coated Nanoconstruct Composed of Gold Nanoparticles Noncovalently Coated with Small Interfering RNA: Preparation, Purification and Characterization
title_sort lipid-coated nanoconstruct composed of gold nanoparticles noncovalently coated with small interfering rna: preparation, purification and characterization
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8624966/
https://www.ncbi.nlm.nih.gov/pubmed/34835540
http://dx.doi.org/10.3390/nano11112775
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