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Distinguishing between Isobaric Ions Using Microdroplet Hydrogen–Deuterium Exchange Mass Spectrometry

Isobaric ions having the same mass-to-charge ratio cannot be separately identified by mass spectrometry (MS) alone, but this limitation can be overcome by using hydrogen–deuterium exchange (HDX) in microdroplets. Because isobaric ions may contain a varied number of exchangeable sites and different t...

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Detalles Bibliográficos
Autores principales: Song, Xiaowei, Li, Jia, Mofidfar, Mohammad, Zare, Richard N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8625015/
https://www.ncbi.nlm.nih.gov/pubmed/34822386
http://dx.doi.org/10.3390/metabo11110728
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author Song, Xiaowei
Li, Jia
Mofidfar, Mohammad
Zare, Richard N.
author_facet Song, Xiaowei
Li, Jia
Mofidfar, Mohammad
Zare, Richard N.
author_sort Song, Xiaowei
collection PubMed
description Isobaric ions having the same mass-to-charge ratio cannot be separately identified by mass spectrometry (MS) alone, but this limitation can be overcome by using hydrogen–deuterium exchange (HDX) in microdroplets. Because isobaric ions may contain a varied number of exchangeable sites and different types of functional groups, each one produces a unique MS spectral pattern after droplet spray HDX without the need for MS/MS experiments or introduction of ion mobility measurements. As an example of the power of this approach, isobaric ions in urinary metabolic profiles are identified and used to distinguish between healthy individuals and those having bladder cancer.
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spelling pubmed-86250152021-11-27 Distinguishing between Isobaric Ions Using Microdroplet Hydrogen–Deuterium Exchange Mass Spectrometry Song, Xiaowei Li, Jia Mofidfar, Mohammad Zare, Richard N. Metabolites Article Isobaric ions having the same mass-to-charge ratio cannot be separately identified by mass spectrometry (MS) alone, but this limitation can be overcome by using hydrogen–deuterium exchange (HDX) in microdroplets. Because isobaric ions may contain a varied number of exchangeable sites and different types of functional groups, each one produces a unique MS spectral pattern after droplet spray HDX without the need for MS/MS experiments or introduction of ion mobility measurements. As an example of the power of this approach, isobaric ions in urinary metabolic profiles are identified and used to distinguish between healthy individuals and those having bladder cancer. MDPI 2021-10-23 /pmc/articles/PMC8625015/ /pubmed/34822386 http://dx.doi.org/10.3390/metabo11110728 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Song, Xiaowei
Li, Jia
Mofidfar, Mohammad
Zare, Richard N.
Distinguishing between Isobaric Ions Using Microdroplet Hydrogen–Deuterium Exchange Mass Spectrometry
title Distinguishing between Isobaric Ions Using Microdroplet Hydrogen–Deuterium Exchange Mass Spectrometry
title_full Distinguishing between Isobaric Ions Using Microdroplet Hydrogen–Deuterium Exchange Mass Spectrometry
title_fullStr Distinguishing between Isobaric Ions Using Microdroplet Hydrogen–Deuterium Exchange Mass Spectrometry
title_full_unstemmed Distinguishing between Isobaric Ions Using Microdroplet Hydrogen–Deuterium Exchange Mass Spectrometry
title_short Distinguishing between Isobaric Ions Using Microdroplet Hydrogen–Deuterium Exchange Mass Spectrometry
title_sort distinguishing between isobaric ions using microdroplet hydrogen–deuterium exchange mass spectrometry
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8625015/
https://www.ncbi.nlm.nih.gov/pubmed/34822386
http://dx.doi.org/10.3390/metabo11110728
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