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Valorization of Fig (Ficus carica L.) Waste Leaves: HPLC-QTOF-MS/MS-DPPH System for Online Screening and Identification of Antioxidant Compounds
Fig (Ficus carica L.) leaves are produced each year and often disposed, resulting in a waste of resources. Fig waste leaves are rich in flavonoids, which have strong antioxidant activity; however, the variety and chemical structure of antioxidants in fig leaves have not been reported in detail. To t...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8625020/ https://www.ncbi.nlm.nih.gov/pubmed/34834895 http://dx.doi.org/10.3390/plants10112532 |
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author | Li, Chunying Yu, Meiting Li, Shen Yang, Xue Qiao, Bin Shi, Sen Zhao, Chunjian Fu, Yujie |
author_facet | Li, Chunying Yu, Meiting Li, Shen Yang, Xue Qiao, Bin Shi, Sen Zhao, Chunjian Fu, Yujie |
author_sort | Li, Chunying |
collection | PubMed |
description | Fig (Ficus carica L.) leaves are produced each year and often disposed, resulting in a waste of resources. Fig waste leaves are rich in flavonoids, which have strong antioxidant activity; however, the variety and chemical structure of antioxidants in fig leaves have not been reported in detail. To take full advantage of fig waste leaves, antioxidant capacity of different extracts (petroleum ether, ethyl acetate, and water) was evaluated by 1, 1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic) acid (ABTS), and ferric-ion-reducing antioxidant power (FRAP) methods. The results showed that flavonoids in ethyl acetate extraction had the highest content (83.92 ± 0.01 mg/g), maximum DPPH scavenging activity (IC(50) 0.54 mg/mL), highest ABTS scavenging rate (80.28%), and FRAP (3.46 mmol/g). Furthermore, an HPLC-QTOF-MS/MS-DPPH method was developed to identify 11 flavonoids in fig waste leaves. This rapid and efficient method can not only be used for screening the antioxidant components in fig waste leaves, but also can be combined with mass spectrometry to identify the compounds with antioxidant capacity. There are three flavonoids with significant antioxidant capacity, which are 3-O-(rhamnopyranosyl-glucopyranosyl)-7-O-(glucopyranosyl)-quercetin, isoschaftoside, and rutin. The results confirmed that fig waste leaves contain a variety of antioxidant components, which contributed to increase the value of fig waste leaves as antioxidants. |
format | Online Article Text |
id | pubmed-8625020 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-86250202021-11-27 Valorization of Fig (Ficus carica L.) Waste Leaves: HPLC-QTOF-MS/MS-DPPH System for Online Screening and Identification of Antioxidant Compounds Li, Chunying Yu, Meiting Li, Shen Yang, Xue Qiao, Bin Shi, Sen Zhao, Chunjian Fu, Yujie Plants (Basel) Article Fig (Ficus carica L.) leaves are produced each year and often disposed, resulting in a waste of resources. Fig waste leaves are rich in flavonoids, which have strong antioxidant activity; however, the variety and chemical structure of antioxidants in fig leaves have not been reported in detail. To take full advantage of fig waste leaves, antioxidant capacity of different extracts (petroleum ether, ethyl acetate, and water) was evaluated by 1, 1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic) acid (ABTS), and ferric-ion-reducing antioxidant power (FRAP) methods. The results showed that flavonoids in ethyl acetate extraction had the highest content (83.92 ± 0.01 mg/g), maximum DPPH scavenging activity (IC(50) 0.54 mg/mL), highest ABTS scavenging rate (80.28%), and FRAP (3.46 mmol/g). Furthermore, an HPLC-QTOF-MS/MS-DPPH method was developed to identify 11 flavonoids in fig waste leaves. This rapid and efficient method can not only be used for screening the antioxidant components in fig waste leaves, but also can be combined with mass spectrometry to identify the compounds with antioxidant capacity. There are three flavonoids with significant antioxidant capacity, which are 3-O-(rhamnopyranosyl-glucopyranosyl)-7-O-(glucopyranosyl)-quercetin, isoschaftoside, and rutin. The results confirmed that fig waste leaves contain a variety of antioxidant components, which contributed to increase the value of fig waste leaves as antioxidants. MDPI 2021-11-21 /pmc/articles/PMC8625020/ /pubmed/34834895 http://dx.doi.org/10.3390/plants10112532 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Li, Chunying Yu, Meiting Li, Shen Yang, Xue Qiao, Bin Shi, Sen Zhao, Chunjian Fu, Yujie Valorization of Fig (Ficus carica L.) Waste Leaves: HPLC-QTOF-MS/MS-DPPH System for Online Screening and Identification of Antioxidant Compounds |
title | Valorization of Fig (Ficus carica L.) Waste Leaves: HPLC-QTOF-MS/MS-DPPH System for Online Screening and Identification of Antioxidant Compounds |
title_full | Valorization of Fig (Ficus carica L.) Waste Leaves: HPLC-QTOF-MS/MS-DPPH System for Online Screening and Identification of Antioxidant Compounds |
title_fullStr | Valorization of Fig (Ficus carica L.) Waste Leaves: HPLC-QTOF-MS/MS-DPPH System for Online Screening and Identification of Antioxidant Compounds |
title_full_unstemmed | Valorization of Fig (Ficus carica L.) Waste Leaves: HPLC-QTOF-MS/MS-DPPH System for Online Screening and Identification of Antioxidant Compounds |
title_short | Valorization of Fig (Ficus carica L.) Waste Leaves: HPLC-QTOF-MS/MS-DPPH System for Online Screening and Identification of Antioxidant Compounds |
title_sort | valorization of fig (ficus carica l.) waste leaves: hplc-qtof-ms/ms-dpph system for online screening and identification of antioxidant compounds |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8625020/ https://www.ncbi.nlm.nih.gov/pubmed/34834895 http://dx.doi.org/10.3390/plants10112532 |
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