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Successive Inoculations of Pigs with Porcine Reproductive and Respiratory Syndrome Virus 1 (PRRSV-1) and Swine H1N2 Influenza Virus Suggest a Mutual Interference between the Two Viral Infections

Porcine reproductive and respiratory syndrome virus (PRRSV) and swine influenza A virus (swIAV) are major pathogens of the porcine respiratory disease complex, but little is known on their interaction in super-infected pigs. In this study, we investigated clinical, virological and immunological outc...

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Autores principales: Bougon, Juliette, Deblanc, Céline, Renson, Patricia, Quéguiner, Stéphane, Gorin, Stéphane, Mahé, Sophie, Le Dimna, Mireille, Barbier, Nicolas, Paboeuf, Frédéric, Simon, Gaëlle, Bourry, Olivier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8625072/
https://www.ncbi.nlm.nih.gov/pubmed/34834975
http://dx.doi.org/10.3390/v13112169
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author Bougon, Juliette
Deblanc, Céline
Renson, Patricia
Quéguiner, Stéphane
Gorin, Stéphane
Mahé, Sophie
Le Dimna, Mireille
Barbier, Nicolas
Paboeuf, Frédéric
Simon, Gaëlle
Bourry, Olivier
author_facet Bougon, Juliette
Deblanc, Céline
Renson, Patricia
Quéguiner, Stéphane
Gorin, Stéphane
Mahé, Sophie
Le Dimna, Mireille
Barbier, Nicolas
Paboeuf, Frédéric
Simon, Gaëlle
Bourry, Olivier
author_sort Bougon, Juliette
collection PubMed
description Porcine reproductive and respiratory syndrome virus (PRRSV) and swine influenza A virus (swIAV) are major pathogens of the porcine respiratory disease complex, but little is known on their interaction in super-infected pigs. In this study, we investigated clinical, virological and immunological outcomes of successive infections with PRRSV-1 and H1N2 swIAV. Twenty-four specific pathogen-free piglets were distributed into four groups and inoculated either with PRRSV at study day (SD) 0, or with swIAV at SD8, or with PRRSV and swIAV one week apart at SD0 and SD8, respectively, or mock-inoculated. In PRRSV/swIAV group, the clinical signs usually observed after swIAV infection were attenuated while higher levels of anti-swIAV antibodies were measured in lungs. Concurrently, PRRSV multiplication in lungs was significantly affected by swIAV infection, whereas the cell-mediated immune response specific to PRRSV was detected earlier in blood, as compared to PRRSV group. Moreover, levels of interferon (IFN)-α measured from SD9 in the blood of super-infected pigs were lower than those measured in the swIAV group, but higher than in the PRRSV group at the same time. Correlation analyses suggested an important role of IFN-α in the two-way interference highlighted between both viral infections.
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spelling pubmed-86250722021-11-27 Successive Inoculations of Pigs with Porcine Reproductive and Respiratory Syndrome Virus 1 (PRRSV-1) and Swine H1N2 Influenza Virus Suggest a Mutual Interference between the Two Viral Infections Bougon, Juliette Deblanc, Céline Renson, Patricia Quéguiner, Stéphane Gorin, Stéphane Mahé, Sophie Le Dimna, Mireille Barbier, Nicolas Paboeuf, Frédéric Simon, Gaëlle Bourry, Olivier Viruses Article Porcine reproductive and respiratory syndrome virus (PRRSV) and swine influenza A virus (swIAV) are major pathogens of the porcine respiratory disease complex, but little is known on their interaction in super-infected pigs. In this study, we investigated clinical, virological and immunological outcomes of successive infections with PRRSV-1 and H1N2 swIAV. Twenty-four specific pathogen-free piglets were distributed into four groups and inoculated either with PRRSV at study day (SD) 0, or with swIAV at SD8, or with PRRSV and swIAV one week apart at SD0 and SD8, respectively, or mock-inoculated. In PRRSV/swIAV group, the clinical signs usually observed after swIAV infection were attenuated while higher levels of anti-swIAV antibodies were measured in lungs. Concurrently, PRRSV multiplication in lungs was significantly affected by swIAV infection, whereas the cell-mediated immune response specific to PRRSV was detected earlier in blood, as compared to PRRSV group. Moreover, levels of interferon (IFN)-α measured from SD9 in the blood of super-infected pigs were lower than those measured in the swIAV group, but higher than in the PRRSV group at the same time. Correlation analyses suggested an important role of IFN-α in the two-way interference highlighted between both viral infections. MDPI 2021-10-27 /pmc/articles/PMC8625072/ /pubmed/34834975 http://dx.doi.org/10.3390/v13112169 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bougon, Juliette
Deblanc, Céline
Renson, Patricia
Quéguiner, Stéphane
Gorin, Stéphane
Mahé, Sophie
Le Dimna, Mireille
Barbier, Nicolas
Paboeuf, Frédéric
Simon, Gaëlle
Bourry, Olivier
Successive Inoculations of Pigs with Porcine Reproductive and Respiratory Syndrome Virus 1 (PRRSV-1) and Swine H1N2 Influenza Virus Suggest a Mutual Interference between the Two Viral Infections
title Successive Inoculations of Pigs with Porcine Reproductive and Respiratory Syndrome Virus 1 (PRRSV-1) and Swine H1N2 Influenza Virus Suggest a Mutual Interference between the Two Viral Infections
title_full Successive Inoculations of Pigs with Porcine Reproductive and Respiratory Syndrome Virus 1 (PRRSV-1) and Swine H1N2 Influenza Virus Suggest a Mutual Interference between the Two Viral Infections
title_fullStr Successive Inoculations of Pigs with Porcine Reproductive and Respiratory Syndrome Virus 1 (PRRSV-1) and Swine H1N2 Influenza Virus Suggest a Mutual Interference between the Two Viral Infections
title_full_unstemmed Successive Inoculations of Pigs with Porcine Reproductive and Respiratory Syndrome Virus 1 (PRRSV-1) and Swine H1N2 Influenza Virus Suggest a Mutual Interference between the Two Viral Infections
title_short Successive Inoculations of Pigs with Porcine Reproductive and Respiratory Syndrome Virus 1 (PRRSV-1) and Swine H1N2 Influenza Virus Suggest a Mutual Interference between the Two Viral Infections
title_sort successive inoculations of pigs with porcine reproductive and respiratory syndrome virus 1 (prrsv-1) and swine h1n2 influenza virus suggest a mutual interference between the two viral infections
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8625072/
https://www.ncbi.nlm.nih.gov/pubmed/34834975
http://dx.doi.org/10.3390/v13112169
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