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Omnipresence of Partitiviruses in Rice Aggregate Sheath Spot Symptom-Associated Fungal Isolates from Paddies in Thailand
Partitiviruses are one of the most prevalent double-stranded RNA viruses that have been identified mostly in filamentous fungi and plants. Partitiviruses generally infect host fungi asymptomatically but infrequently exert significant effect(s) on morphology and virulence, thus being considered a pot...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8625198/ https://www.ncbi.nlm.nih.gov/pubmed/34835075 http://dx.doi.org/10.3390/v13112269 |
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author | Neang, Sokty Bincader, Santiti Rangsuwan, Sansern Keawmanee, Pisut Rin, Soriya Salaipeth, Lakha Das, Subha Kondo, Hideki Suzuki, Nobuhiro Sato, Ikuo Takemoto, Daigo Rattanakreetakul, Chainarong Pongpisutta, Ratiya Arakawa, Masao Chiba, Sotaro |
author_facet | Neang, Sokty Bincader, Santiti Rangsuwan, Sansern Keawmanee, Pisut Rin, Soriya Salaipeth, Lakha Das, Subha Kondo, Hideki Suzuki, Nobuhiro Sato, Ikuo Takemoto, Daigo Rattanakreetakul, Chainarong Pongpisutta, Ratiya Arakawa, Masao Chiba, Sotaro |
author_sort | Neang, Sokty |
collection | PubMed |
description | Partitiviruses are one of the most prevalent double-stranded RNA viruses that have been identified mostly in filamentous fungi and plants. Partitiviruses generally infect host fungi asymptomatically but infrequently exert significant effect(s) on morphology and virulence, thus being considered a potential source of biological control agents against pathogenic fungi. In this study, we performed a screening for mycoviruses of a collection of Thai isolates of rice fungal pathogen Rhizoctonia oryzae-sativae, a causal agent of rice aggregated sheath spot disease. As a result, 36% of tested isolates carried potentially viral double-stranded RNAs with sizes ranging from 2 to 3 kbp. By conventional cDNA library construction and RNA-seq, we determined six new alphapartitiviruses that infected three isolates: tentatively named Rhizoctonia oryzae-sativae partitivirus 1 to 6 (RosPV1-6). Furthermore, RT-PCR detection of each virus revealed their omnipresent nature in different R. oryzae-sativae isolates. Although virus-curing of basidiomycetous fungi is generally difficult, our repeated attempts successfully obtained virus-free (for RosPV1, RosPV2, and uncharacterized partitiviruses), isogenic strain of R. oryzae-sativae TSS190442. The virus-cured strain showed slightly faster colony growth on the synthetic media and severe symptom development on the rice sheath compared to its virus-infected counterpart. Overall, this study shed light on the distribution of partitiviruses in R. oryzae-sativae in a paddy environment and exemplified a virus-curing protocol that may be applicable for other basidiomycetous fungi. |
format | Online Article Text |
id | pubmed-8625198 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-86251982021-11-27 Omnipresence of Partitiviruses in Rice Aggregate Sheath Spot Symptom-Associated Fungal Isolates from Paddies in Thailand Neang, Sokty Bincader, Santiti Rangsuwan, Sansern Keawmanee, Pisut Rin, Soriya Salaipeth, Lakha Das, Subha Kondo, Hideki Suzuki, Nobuhiro Sato, Ikuo Takemoto, Daigo Rattanakreetakul, Chainarong Pongpisutta, Ratiya Arakawa, Masao Chiba, Sotaro Viruses Article Partitiviruses are one of the most prevalent double-stranded RNA viruses that have been identified mostly in filamentous fungi and plants. Partitiviruses generally infect host fungi asymptomatically but infrequently exert significant effect(s) on morphology and virulence, thus being considered a potential source of biological control agents against pathogenic fungi. In this study, we performed a screening for mycoviruses of a collection of Thai isolates of rice fungal pathogen Rhizoctonia oryzae-sativae, a causal agent of rice aggregated sheath spot disease. As a result, 36% of tested isolates carried potentially viral double-stranded RNAs with sizes ranging from 2 to 3 kbp. By conventional cDNA library construction and RNA-seq, we determined six new alphapartitiviruses that infected three isolates: tentatively named Rhizoctonia oryzae-sativae partitivirus 1 to 6 (RosPV1-6). Furthermore, RT-PCR detection of each virus revealed their omnipresent nature in different R. oryzae-sativae isolates. Although virus-curing of basidiomycetous fungi is generally difficult, our repeated attempts successfully obtained virus-free (for RosPV1, RosPV2, and uncharacterized partitiviruses), isogenic strain of R. oryzae-sativae TSS190442. The virus-cured strain showed slightly faster colony growth on the synthetic media and severe symptom development on the rice sheath compared to its virus-infected counterpart. Overall, this study shed light on the distribution of partitiviruses in R. oryzae-sativae in a paddy environment and exemplified a virus-curing protocol that may be applicable for other basidiomycetous fungi. MDPI 2021-11-12 /pmc/articles/PMC8625198/ /pubmed/34835075 http://dx.doi.org/10.3390/v13112269 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Neang, Sokty Bincader, Santiti Rangsuwan, Sansern Keawmanee, Pisut Rin, Soriya Salaipeth, Lakha Das, Subha Kondo, Hideki Suzuki, Nobuhiro Sato, Ikuo Takemoto, Daigo Rattanakreetakul, Chainarong Pongpisutta, Ratiya Arakawa, Masao Chiba, Sotaro Omnipresence of Partitiviruses in Rice Aggregate Sheath Spot Symptom-Associated Fungal Isolates from Paddies in Thailand |
title | Omnipresence of Partitiviruses in Rice Aggregate Sheath Spot Symptom-Associated Fungal Isolates from Paddies in Thailand |
title_full | Omnipresence of Partitiviruses in Rice Aggregate Sheath Spot Symptom-Associated Fungal Isolates from Paddies in Thailand |
title_fullStr | Omnipresence of Partitiviruses in Rice Aggregate Sheath Spot Symptom-Associated Fungal Isolates from Paddies in Thailand |
title_full_unstemmed | Omnipresence of Partitiviruses in Rice Aggregate Sheath Spot Symptom-Associated Fungal Isolates from Paddies in Thailand |
title_short | Omnipresence of Partitiviruses in Rice Aggregate Sheath Spot Symptom-Associated Fungal Isolates from Paddies in Thailand |
title_sort | omnipresence of partitiviruses in rice aggregate sheath spot symptom-associated fungal isolates from paddies in thailand |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8625198/ https://www.ncbi.nlm.nih.gov/pubmed/34835075 http://dx.doi.org/10.3390/v13112269 |
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