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Synthetic Poly(lactic-co-glycolic Acid) Microvesicles as a Feasible Carbon Monoxide-Releasing Platform for Cancer Treatment

Biogenic microvesicles (MVs) play a pivotal role in intercellular signal communication, thus initiating critical biological responses such as the proliferation of cancer cells, gene and protein transport, and chemo-drug resistance. In addition, they have been recognized as having great potential in...

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Autores principales: Wang, Wen-Jyun, Kan, Chung-Dann, Chen, Chih-Yen, Meng, Yi-Yao, Wang, Jieh-Neng, Chen, Wei-Ling, Chen, Chia-Hsiang, Li, Wei-Peng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8625701/
https://www.ncbi.nlm.nih.gov/pubmed/34832047
http://dx.doi.org/10.3390/membranes11110818
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author Wang, Wen-Jyun
Kan, Chung-Dann
Chen, Chih-Yen
Meng, Yi-Yao
Wang, Jieh-Neng
Chen, Wei-Ling
Chen, Chia-Hsiang
Li, Wei-Peng
author_facet Wang, Wen-Jyun
Kan, Chung-Dann
Chen, Chih-Yen
Meng, Yi-Yao
Wang, Jieh-Neng
Chen, Wei-Ling
Chen, Chia-Hsiang
Li, Wei-Peng
author_sort Wang, Wen-Jyun
collection PubMed
description Biogenic microvesicles (MVs) play a pivotal role in intercellular signal communication, thus initiating critical biological responses such as the proliferation of cancer cells, gene and protein transport, and chemo-drug resistance. In addition, they have been recognized as having great potential in drug delivery applications. However, the productivity of biologically produced MVs is not sufficient for clinical applications. In this study, synthetic poly(lactic-co-glycolic acid) (PLGA) MVs were prepared via a double emulsion method. The PLGA MVs had a biogenic MV-mimic vesicular structure with a hydrophilic core/surface and hydrophobic interior of the shell, showing great potential for drug delivery. We successfully embedded hydrophobic iron carbonyl (IC), a carbon monoxide (CO) donor, in the PLGA shell region, enabling the delivery of IC in an aqueous solution. Because of the intrinsic properties of PLGA, it was susceptible to temperature, and the MVs could easily collapse in a warm environment, leading to the decomposition of IC into CO. The in vitro result indicated that the cell viability of A549 lung carcinoma cells significantly decreased to 14% after treatment with IC-loaded PLGA MVs for 24 h, suggesting that these synthetic PLGA MVs constitute an excellent drug delivery platform.
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spelling pubmed-86257012021-11-27 Synthetic Poly(lactic-co-glycolic Acid) Microvesicles as a Feasible Carbon Monoxide-Releasing Platform for Cancer Treatment Wang, Wen-Jyun Kan, Chung-Dann Chen, Chih-Yen Meng, Yi-Yao Wang, Jieh-Neng Chen, Wei-Ling Chen, Chia-Hsiang Li, Wei-Peng Membranes (Basel) Communication Biogenic microvesicles (MVs) play a pivotal role in intercellular signal communication, thus initiating critical biological responses such as the proliferation of cancer cells, gene and protein transport, and chemo-drug resistance. In addition, they have been recognized as having great potential in drug delivery applications. However, the productivity of biologically produced MVs is not sufficient for clinical applications. In this study, synthetic poly(lactic-co-glycolic acid) (PLGA) MVs were prepared via a double emulsion method. The PLGA MVs had a biogenic MV-mimic vesicular structure with a hydrophilic core/surface and hydrophobic interior of the shell, showing great potential for drug delivery. We successfully embedded hydrophobic iron carbonyl (IC), a carbon monoxide (CO) donor, in the PLGA shell region, enabling the delivery of IC in an aqueous solution. Because of the intrinsic properties of PLGA, it was susceptible to temperature, and the MVs could easily collapse in a warm environment, leading to the decomposition of IC into CO. The in vitro result indicated that the cell viability of A549 lung carcinoma cells significantly decreased to 14% after treatment with IC-loaded PLGA MVs for 24 h, suggesting that these synthetic PLGA MVs constitute an excellent drug delivery platform. MDPI 2021-10-26 /pmc/articles/PMC8625701/ /pubmed/34832047 http://dx.doi.org/10.3390/membranes11110818 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Wang, Wen-Jyun
Kan, Chung-Dann
Chen, Chih-Yen
Meng, Yi-Yao
Wang, Jieh-Neng
Chen, Wei-Ling
Chen, Chia-Hsiang
Li, Wei-Peng
Synthetic Poly(lactic-co-glycolic Acid) Microvesicles as a Feasible Carbon Monoxide-Releasing Platform for Cancer Treatment
title Synthetic Poly(lactic-co-glycolic Acid) Microvesicles as a Feasible Carbon Monoxide-Releasing Platform for Cancer Treatment
title_full Synthetic Poly(lactic-co-glycolic Acid) Microvesicles as a Feasible Carbon Monoxide-Releasing Platform for Cancer Treatment
title_fullStr Synthetic Poly(lactic-co-glycolic Acid) Microvesicles as a Feasible Carbon Monoxide-Releasing Platform for Cancer Treatment
title_full_unstemmed Synthetic Poly(lactic-co-glycolic Acid) Microvesicles as a Feasible Carbon Monoxide-Releasing Platform for Cancer Treatment
title_short Synthetic Poly(lactic-co-glycolic Acid) Microvesicles as a Feasible Carbon Monoxide-Releasing Platform for Cancer Treatment
title_sort synthetic poly(lactic-co-glycolic acid) microvesicles as a feasible carbon monoxide-releasing platform for cancer treatment
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8625701/
https://www.ncbi.nlm.nih.gov/pubmed/34832047
http://dx.doi.org/10.3390/membranes11110818
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