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Effects of Low Doses of L-Carnitine Tartrate and Lipid Multi-Particulate Formulated Creatine Monohydrate on Muscle Protein Synthesis in Myoblasts and Bioavailability in Humans and Rodents

The primary objective of this study was to investigate the potential synergy between low doses of L-carnitine tartrate and creatine monohydrate to induce muscle protein synthesis and anabolic pathway activation in primary human myoblasts. In addition, the effects of Lipid multi-particulates (LMP) fo...

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Autores principales: Fielding, Roger A., Rivas, Donato, Grosicki, Gregory J., Ezzyat, Yassine, Ceglia, Lisa, Price, Lori Lyn, Orhan, Cemal, Sahin, Kazim, Fowler, Kelli, White, Tyler, Durkee, Shane, Kritsch, Katja, Bellamine, Aouatef
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8625796/
https://www.ncbi.nlm.nih.gov/pubmed/34836240
http://dx.doi.org/10.3390/nu13113985
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author Fielding, Roger A.
Rivas, Donato
Grosicki, Gregory J.
Ezzyat, Yassine
Ceglia, Lisa
Price, Lori Lyn
Orhan, Cemal
Sahin, Kazim
Fowler, Kelli
White, Tyler
Durkee, Shane
Kritsch, Katja
Bellamine, Aouatef
author_facet Fielding, Roger A.
Rivas, Donato
Grosicki, Gregory J.
Ezzyat, Yassine
Ceglia, Lisa
Price, Lori Lyn
Orhan, Cemal
Sahin, Kazim
Fowler, Kelli
White, Tyler
Durkee, Shane
Kritsch, Katja
Bellamine, Aouatef
author_sort Fielding, Roger A.
collection PubMed
description The primary objective of this study was to investigate the potential synergy between low doses of L-carnitine tartrate and creatine monohydrate to induce muscle protein synthesis and anabolic pathway activation in primary human myoblasts. In addition, the effects of Lipid multi-particulates (LMP) formulation on creatine stability and bioavailability were assessed in rodents and healthy human subjects. When used individually, L-carnitine tartrate at 50 µM and creatine monohydrate at 0.5 µM did not affect myoblast protein synthesis and signaling. However, when combined, they led to a significant increase in protein synthesis. Increased AKT and RPS6 phosphorylation were observed with 50 µM L-carnitine tartrate 5 µM creatine in combination in primary human myoblasts. When Wistar rats were administered creatine with LMP formulation at either 21 or 51 mg/kg, bioavailability was increased by 27% based on the increase in the area under the curve (AUC) at a 51 mg/kg dose compared to without LMP formulation. Tmax and Cmax were unchanged. Finally, in human subjects, a combination of LMP formulated L-carnitine at 500 mg (from L-carnitine tartrate) with LMP formulated creatine at 100, 200, or 500 mg revealed a significant and dose-dependent increase in plasma creatine concentrations. Serum total L-carnitine levels rose in a similar manner in the three combinations. These results suggest that a combination of low doses of L-carnitine tartrate and creatine monohydrate may lead to a significant and synergistic enhancement of muscle protein synthesis and activation of anabolic signaling. In addition, the LMP formulation of creatine improved its bioavailability. L-carnitine at 500 mg and LMP-formulated creatine at 200 or 500 mg may be useful for future clinical trials to evaluate the effects on muscle protein synthesis.
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spelling pubmed-86257962021-11-27 Effects of Low Doses of L-Carnitine Tartrate and Lipid Multi-Particulate Formulated Creatine Monohydrate on Muscle Protein Synthesis in Myoblasts and Bioavailability in Humans and Rodents Fielding, Roger A. Rivas, Donato Grosicki, Gregory J. Ezzyat, Yassine Ceglia, Lisa Price, Lori Lyn Orhan, Cemal Sahin, Kazim Fowler, Kelli White, Tyler Durkee, Shane Kritsch, Katja Bellamine, Aouatef Nutrients Article The primary objective of this study was to investigate the potential synergy between low doses of L-carnitine tartrate and creatine monohydrate to induce muscle protein synthesis and anabolic pathway activation in primary human myoblasts. In addition, the effects of Lipid multi-particulates (LMP) formulation on creatine stability and bioavailability were assessed in rodents and healthy human subjects. When used individually, L-carnitine tartrate at 50 µM and creatine monohydrate at 0.5 µM did not affect myoblast protein synthesis and signaling. However, when combined, they led to a significant increase in protein synthesis. Increased AKT and RPS6 phosphorylation were observed with 50 µM L-carnitine tartrate 5 µM creatine in combination in primary human myoblasts. When Wistar rats were administered creatine with LMP formulation at either 21 or 51 mg/kg, bioavailability was increased by 27% based on the increase in the area under the curve (AUC) at a 51 mg/kg dose compared to without LMP formulation. Tmax and Cmax were unchanged. Finally, in human subjects, a combination of LMP formulated L-carnitine at 500 mg (from L-carnitine tartrate) with LMP formulated creatine at 100, 200, or 500 mg revealed a significant and dose-dependent increase in plasma creatine concentrations. Serum total L-carnitine levels rose in a similar manner in the three combinations. These results suggest that a combination of low doses of L-carnitine tartrate and creatine monohydrate may lead to a significant and synergistic enhancement of muscle protein synthesis and activation of anabolic signaling. In addition, the LMP formulation of creatine improved its bioavailability. L-carnitine at 500 mg and LMP-formulated creatine at 200 or 500 mg may be useful for future clinical trials to evaluate the effects on muscle protein synthesis. MDPI 2021-11-09 /pmc/articles/PMC8625796/ /pubmed/34836240 http://dx.doi.org/10.3390/nu13113985 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Fielding, Roger A.
Rivas, Donato
Grosicki, Gregory J.
Ezzyat, Yassine
Ceglia, Lisa
Price, Lori Lyn
Orhan, Cemal
Sahin, Kazim
Fowler, Kelli
White, Tyler
Durkee, Shane
Kritsch, Katja
Bellamine, Aouatef
Effects of Low Doses of L-Carnitine Tartrate and Lipid Multi-Particulate Formulated Creatine Monohydrate on Muscle Protein Synthesis in Myoblasts and Bioavailability in Humans and Rodents
title Effects of Low Doses of L-Carnitine Tartrate and Lipid Multi-Particulate Formulated Creatine Monohydrate on Muscle Protein Synthesis in Myoblasts and Bioavailability in Humans and Rodents
title_full Effects of Low Doses of L-Carnitine Tartrate and Lipid Multi-Particulate Formulated Creatine Monohydrate on Muscle Protein Synthesis in Myoblasts and Bioavailability in Humans and Rodents
title_fullStr Effects of Low Doses of L-Carnitine Tartrate and Lipid Multi-Particulate Formulated Creatine Monohydrate on Muscle Protein Synthesis in Myoblasts and Bioavailability in Humans and Rodents
title_full_unstemmed Effects of Low Doses of L-Carnitine Tartrate and Lipid Multi-Particulate Formulated Creatine Monohydrate on Muscle Protein Synthesis in Myoblasts and Bioavailability in Humans and Rodents
title_short Effects of Low Doses of L-Carnitine Tartrate and Lipid Multi-Particulate Formulated Creatine Monohydrate on Muscle Protein Synthesis in Myoblasts and Bioavailability in Humans and Rodents
title_sort effects of low doses of l-carnitine tartrate and lipid multi-particulate formulated creatine monohydrate on muscle protein synthesis in myoblasts and bioavailability in humans and rodents
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8625796/
https://www.ncbi.nlm.nih.gov/pubmed/34836240
http://dx.doi.org/10.3390/nu13113985
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