What Could Arrest an Eriophyoid Mite on a Plant? The Case of Aculops allotrichus from the Black Locust Tree

SIMPLE SUMMARY: For a phytophagous arthropod, plant cues and the presence of conspecifics or heterospecifics on a plant are important sources of information about food availability and predation risk. In eriophyoid mites, which are a group of economically important plant parasites, this issue is still poorly understood. In the previous study, females of the eriophyoid Aculops allotrichus prolonged their stay on unprofitable, old black locust leaves with injured conspecifics, suggesting their specific attraction to pierced individuals. The aim of this study was to shed light on this phenomenon. The series of tests using intact and artificially injured conspecifics, heterospecifics, pollen and sand grains revealed the high specificity of cues associated with mite injury. Only the presence of conspecifics triggered female arrestment on unprofitable old leaves, and this effect was more pronounced on leaf patches with injured than with intact eriophyoids. To our best knowledge, A. allotrichus is the first described herbivore in which injured conspecifics, instead of causing an alarm to be raised, keep the foraging individuals within a risky patch. Our tests using three non-host plants also suggest that A. allotrichus presumably needs more time to identify and evaluate non-host plants than host plants. ABSTRACT: Aculops allotrichus is a vagrant eriophyoid that lives gregariously on the leaves of the black locust tree. This study demonstrated that conspecifics can have a significant impact on A. allotrichus females on unprofitable, old black locust leaves and can arrest them on those leaves. The effect was more pronounced in females that were exposed to artificially injured individuals than to intact ones. They not only prolonged their sojourn on leaf discs with pierced conspecifics, but also preferred the leaf disc halves with damaged individuals to clean ones. Aculops allotrichus is the first described herbivore in which artificially injured conspecifics, instead of causing alarm, keep the foraging individuals within a risky patch. Other objects, such as artificially injured or intact heterospecifics, pollen or sand, were irrelevant to the eriophyoid females on old leaf patches. In tests with old leaves of maple, magnolia and hard kiwi vine, the females postponed their movement from non-host leaf discs, which suggests that they may need more time to recognise and evaluate unfamiliar plants than familiar ones.