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The Use of a Diode Laser for Removal of Microorganisms from the Surfaces of Zirconia and Porcelain Applied to Superstructure Dental Implants
The aim of this paper was to study the effectiveness of a diode laser (LD) for removal of microorganisms isolated from porcelain and zirconia crown surfaces used in implantoprosthetics in order to minimize infections around dental implants. In order to optimize biocidal efficacy of the process (at t...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8625943/ https://www.ncbi.nlm.nih.gov/pubmed/34835484 http://dx.doi.org/10.3390/microorganisms9112359 |
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author | Wawrzyk, Anna Łobacz, Michał Adamczuk, Agnieszka Sofińska-Chmiel, Weronika Wilczyński, Sławomir Rahnama, Mansur |
author_facet | Wawrzyk, Anna Łobacz, Michał Adamczuk, Agnieszka Sofińska-Chmiel, Weronika Wilczyński, Sławomir Rahnama, Mansur |
author_sort | Wawrzyk, Anna |
collection | PubMed |
description | The aim of this paper was to study the effectiveness of a diode laser (LD) for removal of microorganisms isolated from porcelain and zirconia crown surfaces used in implantoprosthetics in order to minimize infections around dental implants. In order to optimize biocidal efficacy of the process (at the same time, avoiding increasing the surface roughness during decontamination) the effects of diode laser doses were investigated. The irradiation was performed with a diode laser at the wavelength of λ = 810 nm in three variants with a different number of repetitions (1 × 15 s, 2 × 15 s, 3 × 15 s). The quantitative microbial contamination of the surface of teeth, porcelain and zirconia crowns assessment was made using the culture-dependent method. The identification of microorganisms took place using the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and next-generation sequencing (NGS) methods. The studies of the surface morphology and roughness were carried out by means of the optical profilometry, scanning electron microscopy (SEM) and optical microscopy with the C1 confocal attachment. The most important conclusion from the research is the fact that the laser operation, regardless of the exposure time, effectively eliminates the microorganisms from the surfaces used for dental implant rebuilding and does not have a destructive effect on the tested material. |
format | Online Article Text |
id | pubmed-8625943 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-86259432021-11-27 The Use of a Diode Laser for Removal of Microorganisms from the Surfaces of Zirconia and Porcelain Applied to Superstructure Dental Implants Wawrzyk, Anna Łobacz, Michał Adamczuk, Agnieszka Sofińska-Chmiel, Weronika Wilczyński, Sławomir Rahnama, Mansur Microorganisms Article The aim of this paper was to study the effectiveness of a diode laser (LD) for removal of microorganisms isolated from porcelain and zirconia crown surfaces used in implantoprosthetics in order to minimize infections around dental implants. In order to optimize biocidal efficacy of the process (at the same time, avoiding increasing the surface roughness during decontamination) the effects of diode laser doses were investigated. The irradiation was performed with a diode laser at the wavelength of λ = 810 nm in three variants with a different number of repetitions (1 × 15 s, 2 × 15 s, 3 × 15 s). The quantitative microbial contamination of the surface of teeth, porcelain and zirconia crowns assessment was made using the culture-dependent method. The identification of microorganisms took place using the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and next-generation sequencing (NGS) methods. The studies of the surface morphology and roughness were carried out by means of the optical profilometry, scanning electron microscopy (SEM) and optical microscopy with the C1 confocal attachment. The most important conclusion from the research is the fact that the laser operation, regardless of the exposure time, effectively eliminates the microorganisms from the surfaces used for dental implant rebuilding and does not have a destructive effect on the tested material. MDPI 2021-11-15 /pmc/articles/PMC8625943/ /pubmed/34835484 http://dx.doi.org/10.3390/microorganisms9112359 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wawrzyk, Anna Łobacz, Michał Adamczuk, Agnieszka Sofińska-Chmiel, Weronika Wilczyński, Sławomir Rahnama, Mansur The Use of a Diode Laser for Removal of Microorganisms from the Surfaces of Zirconia and Porcelain Applied to Superstructure Dental Implants |
title | The Use of a Diode Laser for Removal of Microorganisms from the Surfaces of Zirconia and Porcelain Applied to Superstructure Dental Implants |
title_full | The Use of a Diode Laser for Removal of Microorganisms from the Surfaces of Zirconia and Porcelain Applied to Superstructure Dental Implants |
title_fullStr | The Use of a Diode Laser for Removal of Microorganisms from the Surfaces of Zirconia and Porcelain Applied to Superstructure Dental Implants |
title_full_unstemmed | The Use of a Diode Laser for Removal of Microorganisms from the Surfaces of Zirconia and Porcelain Applied to Superstructure Dental Implants |
title_short | The Use of a Diode Laser for Removal of Microorganisms from the Surfaces of Zirconia and Porcelain Applied to Superstructure Dental Implants |
title_sort | use of a diode laser for removal of microorganisms from the surfaces of zirconia and porcelain applied to superstructure dental implants |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8625943/ https://www.ncbi.nlm.nih.gov/pubmed/34835484 http://dx.doi.org/10.3390/microorganisms9112359 |
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