Cargando…

The Use of a Diode Laser for Removal of Microorganisms from the Surfaces of Zirconia and Porcelain Applied to Superstructure Dental Implants

The aim of this paper was to study the effectiveness of a diode laser (LD) for removal of microorganisms isolated from porcelain and zirconia crown surfaces used in implantoprosthetics in order to minimize infections around dental implants. In order to optimize biocidal efficacy of the process (at t...

Descripción completa

Detalles Bibliográficos
Autores principales: Wawrzyk, Anna, Łobacz, Michał, Adamczuk, Agnieszka, Sofińska-Chmiel, Weronika, Wilczyński, Sławomir, Rahnama, Mansur
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8625943/
https://www.ncbi.nlm.nih.gov/pubmed/34835484
http://dx.doi.org/10.3390/microorganisms9112359
_version_ 1784606545255858176
author Wawrzyk, Anna
Łobacz, Michał
Adamczuk, Agnieszka
Sofińska-Chmiel, Weronika
Wilczyński, Sławomir
Rahnama, Mansur
author_facet Wawrzyk, Anna
Łobacz, Michał
Adamczuk, Agnieszka
Sofińska-Chmiel, Weronika
Wilczyński, Sławomir
Rahnama, Mansur
author_sort Wawrzyk, Anna
collection PubMed
description The aim of this paper was to study the effectiveness of a diode laser (LD) for removal of microorganisms isolated from porcelain and zirconia crown surfaces used in implantoprosthetics in order to minimize infections around dental implants. In order to optimize biocidal efficacy of the process (at the same time, avoiding increasing the surface roughness during decontamination) the effects of diode laser doses were investigated. The irradiation was performed with a diode laser at the wavelength of λ = 810 nm in three variants with a different number of repetitions (1 × 15 s, 2 × 15 s, 3 × 15 s). The quantitative microbial contamination of the surface of teeth, porcelain and zirconia crowns assessment was made using the culture-dependent method. The identification of microorganisms took place using the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and next-generation sequencing (NGS) methods. The studies of the surface morphology and roughness were carried out by means of the optical profilometry, scanning electron microscopy (SEM) and optical microscopy with the C1 confocal attachment. The most important conclusion from the research is the fact that the laser operation, regardless of the exposure time, effectively eliminates the microorganisms from the surfaces used for dental implant rebuilding and does not have a destructive effect on the tested material.
format Online
Article
Text
id pubmed-8625943
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-86259432021-11-27 The Use of a Diode Laser for Removal of Microorganisms from the Surfaces of Zirconia and Porcelain Applied to Superstructure Dental Implants Wawrzyk, Anna Łobacz, Michał Adamczuk, Agnieszka Sofińska-Chmiel, Weronika Wilczyński, Sławomir Rahnama, Mansur Microorganisms Article The aim of this paper was to study the effectiveness of a diode laser (LD) for removal of microorganisms isolated from porcelain and zirconia crown surfaces used in implantoprosthetics in order to minimize infections around dental implants. In order to optimize biocidal efficacy of the process (at the same time, avoiding increasing the surface roughness during decontamination) the effects of diode laser doses were investigated. The irradiation was performed with a diode laser at the wavelength of λ = 810 nm in three variants with a different number of repetitions (1 × 15 s, 2 × 15 s, 3 × 15 s). The quantitative microbial contamination of the surface of teeth, porcelain and zirconia crowns assessment was made using the culture-dependent method. The identification of microorganisms took place using the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and next-generation sequencing (NGS) methods. The studies of the surface morphology and roughness were carried out by means of the optical profilometry, scanning electron microscopy (SEM) and optical microscopy with the C1 confocal attachment. The most important conclusion from the research is the fact that the laser operation, regardless of the exposure time, effectively eliminates the microorganisms from the surfaces used for dental implant rebuilding and does not have a destructive effect on the tested material. MDPI 2021-11-15 /pmc/articles/PMC8625943/ /pubmed/34835484 http://dx.doi.org/10.3390/microorganisms9112359 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wawrzyk, Anna
Łobacz, Michał
Adamczuk, Agnieszka
Sofińska-Chmiel, Weronika
Wilczyński, Sławomir
Rahnama, Mansur
The Use of a Diode Laser for Removal of Microorganisms from the Surfaces of Zirconia and Porcelain Applied to Superstructure Dental Implants
title The Use of a Diode Laser for Removal of Microorganisms from the Surfaces of Zirconia and Porcelain Applied to Superstructure Dental Implants
title_full The Use of a Diode Laser for Removal of Microorganisms from the Surfaces of Zirconia and Porcelain Applied to Superstructure Dental Implants
title_fullStr The Use of a Diode Laser for Removal of Microorganisms from the Surfaces of Zirconia and Porcelain Applied to Superstructure Dental Implants
title_full_unstemmed The Use of a Diode Laser for Removal of Microorganisms from the Surfaces of Zirconia and Porcelain Applied to Superstructure Dental Implants
title_short The Use of a Diode Laser for Removal of Microorganisms from the Surfaces of Zirconia and Porcelain Applied to Superstructure Dental Implants
title_sort use of a diode laser for removal of microorganisms from the surfaces of zirconia and porcelain applied to superstructure dental implants
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8625943/
https://www.ncbi.nlm.nih.gov/pubmed/34835484
http://dx.doi.org/10.3390/microorganisms9112359
work_keys_str_mv AT wawrzykanna theuseofadiodelaserforremovalofmicroorganismsfromthesurfacesofzirconiaandporcelainappliedtosuperstructuredentalimplants
AT łobaczmichał theuseofadiodelaserforremovalofmicroorganismsfromthesurfacesofzirconiaandporcelainappliedtosuperstructuredentalimplants
AT adamczukagnieszka theuseofadiodelaserforremovalofmicroorganismsfromthesurfacesofzirconiaandporcelainappliedtosuperstructuredentalimplants
AT sofinskachmielweronika theuseofadiodelaserforremovalofmicroorganismsfromthesurfacesofzirconiaandporcelainappliedtosuperstructuredentalimplants
AT wilczynskisławomir theuseofadiodelaserforremovalofmicroorganismsfromthesurfacesofzirconiaandporcelainappliedtosuperstructuredentalimplants
AT rahnamamansur theuseofadiodelaserforremovalofmicroorganismsfromthesurfacesofzirconiaandporcelainappliedtosuperstructuredentalimplants
AT wawrzykanna useofadiodelaserforremovalofmicroorganismsfromthesurfacesofzirconiaandporcelainappliedtosuperstructuredentalimplants
AT łobaczmichał useofadiodelaserforremovalofmicroorganismsfromthesurfacesofzirconiaandporcelainappliedtosuperstructuredentalimplants
AT adamczukagnieszka useofadiodelaserforremovalofmicroorganismsfromthesurfacesofzirconiaandporcelainappliedtosuperstructuredentalimplants
AT sofinskachmielweronika useofadiodelaserforremovalofmicroorganismsfromthesurfacesofzirconiaandporcelainappliedtosuperstructuredentalimplants
AT wilczynskisławomir useofadiodelaserforremovalofmicroorganismsfromthesurfacesofzirconiaandporcelainappliedtosuperstructuredentalimplants
AT rahnamamansur useofadiodelaserforremovalofmicroorganismsfromthesurfacesofzirconiaandporcelainappliedtosuperstructuredentalimplants